Water is essential for maintaining plant health and optimal growth in agriculture. While some crops depend on irrigation, others can rely on rainfed water, depending on regional climatic conditions. This is exemplified by grapevines, which have specific water level requirements, and irrigation systems are needed. However, these systems can be susceptible to damage or leaks, which are not always easy to detect, requiring meticulous and time-consuming inspection. This study presents a methodology for identifying potential damage or leaks in vineyard irrigation systems using RGB and thermal infrared (TIR) imagery acquired by unmanned aerial vehicles (UAVs). The RGB imagery was used to distinguish between grapevine and non-grapevine pixels, enabling the division of TIR data into three raster products: temperature from grapevines, from non-grapevine areas, and from the entire evaluated vineyard plot. By analyzing the mean temperature values from equally spaced row sections, different threshold values were calculated to estimate and map potential leaks. These thresholds included the lower quintile value, the mean temperature minus the standard deviation (Tmean−σ), and the mean temperature minus two times the standard deviation (Tmean−2σ). The lower quintile threshold showed the best performance in identifying known leak areas and highlighting the closest rows that need inspection in the field. This approach presents a promising solution for inspecting vineyard irrigation systems. By using UAVs, larger areas can be covered on-demand, improving the efficiency and scope of the inspection process. This not only reduces water wastage in viticulture and eases grapevine water stress but also optimizes viticulture practices.