“…Seven days after application of the nail varnish, each participant returned and both hands were prepared using the bag immersion technique (Chou et al., 2011; Incoll et al., 2009) with 10% povidone-iodine (Betadine, Purdue Pharma L.T., Stamford, CT, USA) for 2 minutes as measured by a timer. The bag was carefully removed and the limb allowed to air dry for a further 3 minutes after which sampling was performed.…”