Comparing the ability of luminex xMAP<sup>®</sup>salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population

Liang, Dawei; Jie-hong, LU; Wu, Qi; Ke, Bixia; Jiang, Chang-Hong; Long, Jun; Fang, Yanping; Lin, Lijuan; Zeng, Nianyi; Fu, Li-Ying; Jiang, Lingxiao

doi:10.1111/jam.13106

Cited by 7 publications

(2 citation statements)

References 29 publications

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“…Based on the manufacturer's information sheet, 74 serovars can be fully typed while 26 can be partially typed by O or H antigens. Although SSA has been tested in a number of studies from other countries (Dunbar and Jacobson, 2007 ; Dunbar et al, 2015 ; Liang et al, 2016 ; Yoshida C. et al, 2016 ; Zheng et al, 2017 ), the discrepancies were not well resolved by other molecular methods in previous studies. In this study, we employed WGS and SISTR to resolve the discrepancies between SAT and SSA (Yoshida C. E. et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we evaluated the performance of the xMAP SSA in comparison to the traditional SAT on 255 isolates from human and environmental or food samples from Zhejiang Province of southeast China. We selected all the serovars in our database to test the typeability of serovars in our collection by SSA, including Paratyphi A which was not tested in previous study in southern China (Liang et al, 2016 ) but was a common serovar in China (Lu et al, 2017 ; Qian et al, 2020 ). In this study, we had tested 30 serovars that are listed in the xMAP SSA information sheet as typable, of which 14 serovars were fully typed and 16 serovars were partially typed.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of xMAP Salmonella Serotyping Assay With Traditional Serotyping and Discordance Resolution by Whole Genome Sequencing

Luo

Huang

et al. 2020

Front. Cell. Infect. Microbiol.

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Salmonella spp. are a major cause of foodborne illness throughout the world. Traditional serotyping by antisera agglutination has been used as a standard identification method for many years but newer nucleic acid-based tests have become available that may provide advantages in workflow and test turnaround time. In this study, we evaluated the Luminex ® xMAP ® Salmonella Serotyping Assay (SSA), a multiplex nucleic acid test capable of identifying 85% of the most common Salmonella serotypes, in comparison to the traditional serum agglutination test (SAT) on 4 standard strains and 255 isolates from human (224), environmental, and food (31) samples. Of the total of 259 isolates, 256 could be typed by the SSA. Of these, 197 (77.0%) were fully typed and 59 (23.0%) were partially typed. By SAT, 246 of the 259 isolates (95%) were successfully typed. Sixty isolates had discrepant results between SAT and SSA and were resolved using whole genome sequencing (WGS). By SAT, 80.0% (48/60) of the isolates were consistent with WGS while by SSA 91.7% (55/60) were partially consistent with WGS. By serovar, all 30 serovars except one tested were fully or partially typable. The workflow comparison showed that SSA provided advantages over SAT with a hands-on time (HOT) of 3.5 min and total turnaround time (TAT) of 6 h, as compared to 1 h HOT and 2-6 days TAT for SAT. Overall, this study showed that molecular serotyping is promising as a rapid method for Salmonella serotyping with good accuracy for typing most common Salmonella serovars circulating in China.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of xMAP Salmonella Serotyping Assay With Traditional Serotyping and Discordance Resolution by Whole Genome Sequencing

Luo

Huang

et al. 2020

Front. Cell. Infect. Microbiol.

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Molecular identification of common Salmonella serovars using multiplex DNA sensor-based suspension array

et al. 2018

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Salmonella is one of major foodborne pathogens and the leading cause of foodborne illness-related hospitalizations and deaths. It is critical to develop a sensitive and rapid detection assay that can identify Salmonella to ensure food safety. In this study, a DNA sensor-based suspension array system of high multiplexing ability was developed to identify eight Salmonella serovars commonly associated with foodborne outbreaks to the serotype level. Each DNA sensor was prepared by activating pre-encoded microspheres with oligonucleotide probes that are targeting virulence genes and serovar-specific regions. The mixture of 12 different types of DNA sensors were loaded into a 96-well microplate and used as a 12-plex DNA sensor array platform. DNA isolated from Salmonella was amplified by multiplex polymerase chain reaction (mPCR), and the presence of Salmonella was determined by reading fluorescent signals from hybridization between probes on DNA sensors and fluorescently labeled target DNA using the Bio-Plex® system. The developed multiplex array was able to detect synthetic DNA at the concentration as low as 100 fM and various Salmonella serovars as low as 100 CFU/mL within 1 h post-PCR. Sensitivity of this assay was further improved to 1 CFU/mL with 6-h enrichment. The array system also correctly and specifically identified serotype of tested Salmonella strains without any cross-reactivity with other common foodborne pathogens. Our results indicate the developed DNA sensor suspension array can be a rapid and reliable high-throughput method for simultaneous detection and molecular identification of common Salmonella serotypes.

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A genoserotyping system for a fast and objective identification of Salmonella serotypes commonly isolated from poultry and pork food sectors in Belgium

et al. 2020

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Comparing the ability of luminex xMAP^®salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population

Abstract: The xMAP SSA, with high-throughput characteristics, provides an accurate and rapid serotyping system that dramatically strengthens the capability of clinical and public health laboratories for Salmonella serotyping.

Cited by 7 publications

References 29 publications

Comparison of xMAP Salmonella Serotyping Assay With Traditional Serotyping and Discordance Resolution by Whole Genome Sequencing

Comparison of xMAP Salmonella Serotyping Assay With Traditional Serotyping and Discordance Resolution by Whole Genome Sequencing

Molecular identification of common Salmonella serovars using multiplex DNA sensor-based suspension array

A genoserotyping system for a fast and objective identification of Salmonella serotypes commonly isolated from poultry and pork food sectors in Belgium

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Comparing the ability of luminex xMAP®salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population

Abstract: The xMAP SSA, with high-throughput characteristics, provides an accurate and rapid serotyping system that dramatically strengthens the capability of clinical and public health laboratories for Salmonella serotyping.

Cited by 7 publications

References 29 publications

Comparison of xMAP Salmonella Serotyping Assay With Traditional Serotyping and Discordance Resolution by Whole Genome Sequencing

Comparison of xMAP Salmonella Serotyping Assay With Traditional Serotyping and Discordance Resolution by Whole Genome Sequencing

Molecular identification of common Salmonella serovars using multiplex DNA sensor-based suspension array

A genoserotyping system for a fast and objective identification of Salmonella serotypes commonly isolated from poultry and pork food sectors in Belgium

Contact Info

Product

Resources

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Comparing the ability of luminex xMAP^®salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population