Comparing the effect of cinnamaldehyde and metformin on expression of MiR320 and MiR26-b in insulin resistant 3T3L1 adipocytes

Naghiaee, Yousof; Vakili, Mahmood; Mohammadi, Mohammad; Mohiti, Azra; Mohiti-Ardakani, Javad

doi:10.1016/j.phyplu.2021.100122

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…In this experiment, inducing insulin resistance diminished GLUT4 expression, indicating suppression of the PI3K/AKT pathway. Similar to other studies [ 41 , 42 ] suppression was reverted incompletely by metformin treatment delineating various mechanisms of metformin for exerting its antidiabetic effects. The effects of methanolic and total extracts of PSC were even more pronounced in upregulation of GLUT4, as it far exceeded the level of expression in control cells.…”

Section: Discussionsupporting

confidence: 87%

Paliurus spina-christi Mill fruit extracts improve glucose uptake and activate the insulin signaling pathways in HepG2 insulin-resistant cells

Esfahani

Tarighi

Dianat

et al. 2023

BMC Complement Med Ther

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Background Paliurus spina-christi Mill. (PSC) fruit is frequently used in the treatment of diabetes mellitus in Mediterranean regions. Here, we investigated the effects of various PSC fruit extracts (PSC-FEs) on glucose consumption and some key mediators of insulin signaling pathways in high glucose and high insulin-induced insulin-resistant HepG2 cells. Methods The effects of methanolic, chloroform and total extracts on cell proliferation were assessed by the MTT assay. The potential of non-toxic extracts on glucose utilization in insulin-resistant HepG2 cells was checked using a glucose oxidase assay. AKT and AMP-activated protein kinase (AMPK) pathway activation and mRNA expression levels of insulin receptor (INSR), glucose transporter 1 (GLUT1), and glucose transporters 4 (GLUT4) were determined by western blotting and real-time PCR, respectively. Results We found that high concentrations of methanolic and both low and high concentrations of total extracts were able to enhance glucose uptake in an insulin-resistant cell line model. Moreover, AKT and AMPK phosphorylation were significantly increased by the high strength of methanolic extract, while total extract raised AMPK activation at low and high concentrations. Also, GLUT 1, GLUT 4, and INSR were elevated by both methanolic and total extracts. Conclusions Ultimately, our results shed new light on methanolic and total PSC-FEs as sources of potential anti-diabetic medications, restoring glucose consumption and uptake in insulin-resistant HepG2 cells. These could be at least in part due to re-activating AKT and AMPK signaling pathways and also increased expression of INSR, GLUT1, and GLUT4. Overall, active constituents present in methanolic and total extracts of PCS are appropriate anti-diabetic agents and explain the use of these PSC fruits in traditional medicine for the treatment of diabetes.

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Section: Discussionsupporting

confidence: 87%

Paliurus spina-christi Mill fruit extracts improve glucose uptake and activate the insulin signaling pathways in HepG2 insulin-resistant cells

Esfahani

Tarighi

Dianat

et al. 2023

BMC Complement Med Ther

View full text Add to dashboard Cite

show abstract

“…In this experiment, inducing insulin resistance diminished GLUT4 expression, indicating suppression of the PI3K/AKT pathway. Similar to other studies (42,43) suppression was reverted incompletely by metformin treatment delineating various mechanisms of metformin for exerting its antidiabetic effects. The effects of methanolic and total extracts of PSC were even more pronounced in upregulation of GLUT4, as it far exceeded the level of expression in control cells.…”

Section: Discussionsupporting

confidence: 85%

Paliurus spina-christi fruit extracts improve glucose uptake and activate the insulin signaling pathways in HepG2 insulin-resistant cells

Esfahani

Tarighi

Dianat

et al. 2022

Preprint

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Background: Paliurus spina-christi(PSC) fruit is frequently used in the treatment of diabetes mellitus in Mediterranean regions. Here, we investigated the effects of various PSC fruit extracts (PSC-FE) on glucose consumption and some key mediators of insulin signaling pathways in high glucose and high insulin-induced insulin-resistant HepG2 cells. Methods: The effects of methanolic, chloroform and total extracts on cell proliferation were assessed by the MTT assay. The potential of non-toxic extracts on glucose utilization in insulin-resistant HepG2 cells was checked using a glucose oxidase assay. AKT and AMPK pathway activation and mRNA expression levels of insulin receptor (INSR), GLUT1, and GLUT4 were determined by western blotting and real-time PCR, respectively. Results: We found that high concentrations of methanolic and both low and high concentrations of total extracts were able to enhance glucose uptake in an insulin-resistant cell line model. Moreover, AKT and AMPK phosphorylation were significantly increased by the high strength of methanolic extract, while total extract raised AMPK activation at low and high concentrations. Also, GLUT 1, GLUT 4, and INSR were elevated by both methanolic and total extracts. Conclusions: These results indicate that methanolic and total PSC-FE can restore glucose consumption and uptake in insulin-resistant HepG2 cells, which could be at least in part due to re-activating AKT and AMPK signaling pathways and also increased expression of INSR and glucose transporters. Overall, our study suggests methanolic and total extracts of PCS as appropriate antidiabetic agents and explains the use of these PSC fruits in traditional medicine for the treatment of diabetes.

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Biological fate, functional properties, and design strategies for oral delivery systems for cinnamaldehyde

Weng,

Ho,

2024

Food Funct.

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Comparing the effect of cinnamaldehyde and metformin on expression of MiR320 and MiR26-b in insulin resistant 3T3L1 adipocytes

Cited by 5 publications

References 32 publications

Paliurus spina-christi Mill fruit extracts improve glucose uptake and activate the insulin signaling pathways in HepG2 insulin-resistant cells

Paliurus spina-christi Mill fruit extracts improve glucose uptake and activate the insulin signaling pathways in HepG2 insulin-resistant cells

Paliurus spina-christi fruit extracts improve glucose uptake and activate the insulin signaling pathways in HepG2 insulin-resistant cells

Biological fate, functional properties, and design strategies for oral delivery systems for cinnamaldehyde

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