Comparing the effectiveness of egg disinfectants against bacteria and mitotic indices of developing chick embryos

Zeweil, H. S.; Rizk, R. E.; Bekhet, Gamal M.; Ahmed, Mona R.M.

doi:10.1016/j.jobaz.2014.12.005

Cited by 33 publications

(21 citation statements)

References 38 publications

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“…Eggshell contamination by total aerobic mesophilic bacteria, before any disinfection procedure, varied between 3.17 and 3.57 log10 CFU mL -1 , corroborating the results observed by Coufal et al (2003) for eggs collected from nests. However, values between 4.0 and 7.0 log10 CFU per clean egg were also described by some authors (Wells et al, 2011;Zeweil et al, 2015), which shows how the values for the initial contamination of the eggshell vary greatly between studies. Regardless of the microbial challenge to which the eggs were subjected to before disinfection, the treatments with formaldehyde and UV were effective in reducing the contamination of the eggshell by total aerobic mesophilic bacteria in 2.0 and 1.0 log10 CFU mL -1 , respectively.…”

Section: Resultsmentioning

confidence: 65%

“…Even eggs from clean nests have a certain amount of microorganisms on the eggshell that, under favorable growth conditions, may compromise the hatchability and health of neonatal chicks. On these eggshells, total aerobic mesophilic bacteria count can reach 3.75 to 7.07 log 10 colony-forming units (CFUs) per egg (Coufal et al, 2003;Zeweil et al, 2015). Therefore, reducing the eggshell microbial load through disinfection procedures would improve the quality of the egg to be incubated and reduce the incidence of bacterial infections in embryos and neonatal chicks (Fasenko et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Eggshell microbiology and quality of hatching eggs subjected to different sanitizing procedures

Clímaco

Melo

Vaz

et al. 2018

Pesq. agropec. bras.

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The objective of this work was to evaluate the effect of different disinfection procedures as alternatives to formaldehyde fumigation on eggshell microbial load and quality of eggs from a 42-week-old Cobb commercial breeder flock. A total of 10,080 clean eggs collected from the nests were randomly distributed in a randomized complete block design, among the following treatment groups: 13.33 g m-3 formaldehyde fumigation, 5-10 ppm ozone fumigation, 6.36 mW cm-2 UV-C light irradiation, spraying with 1.56% hydrogen peroxide, spraying with 0.13% peracetic acid, spraying with water (wet control), and no disinfection procedure (dry control). Per treatment, eight samples of four eggs each were collected before and after the disinfection procedure, in order to count the number of Enterobacteriaceae and total aerobic mesophilic bacteria on the eggshell. Only eggs subjected to the formaldehyde and UV treatments showed a significant reduction in total aerobic mesophilic bacterial count on the eggshell, when compared with those of the dry control group. Treatments did not affect eggshell thickness and resistance force. UV light exposure is effective in reducing microbial load on 42-week-old breeder flock eggshells, without affecting their quality, and can be considered an alternative to formaldehyde disinfection.

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Section: Resultsmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Eggshell microbiology and quality of hatching eggs subjected to different sanitizing procedures

Clímaco

Melo

Vaz

et al. 2018

Pesq. agropec. bras.

View full text Add to dashboard Cite

show abstract

“…A menor eclodibilidade absoluta dos ovos do grupo controle (+) quando comparados aos ovos UV 3'30" também pode ser devida aos efeitos do desinfetante sobre os embriões, uma vez que alguns produtos químicos podem ser demasiado tóxicos, além de afetar a cutícula e as propriedades da casca do ovo, prejudicando o desenvolvimento embrionário (Zeweil et al, 2015). Os efeitos tóxicos do paraformaldeído já são reconhecidos há anos (Leeson e Summers, 2000;Turtoi e Borda, 2014;Zeweil et al, 2015) constataram que aves provenientes de desinfecção com esse composto tiveram menor peso, retardo no desenvolvimento e baixas taxas mitóticas em sistema nervoso central e cutâneo durante a incubação em comparação ao uso de soluções desinfetantes naturais.…”

Section: Resultsunclassified

“…Devido à natureza da limpeza com esponjas de aço realizada nos ovos sujos do presente experimento, a camada cuticular pode ter sido afetada de maneira a controlar menos eficientemente as trocas gasosas e líquidas entre o interior do ovo e o ambiente. Tal controle proporcionado pela casca é responsável pela manutenção de uma temperatura ótima para o embrião, pela perda controlada de peso do ovo e pelas trocas gasosas de oxigênio e gás carbônico entre seu interior e exterior (Simons e Wiertz, 1966;Molenaar et al, 2010;King'ori, 2012;Zeweil et al, 2015). Wells et al (2010) verificaram em seu experimento que um maior tempo de exposição ultravioleta causou superaquecimento do interior dos ovos, atingindo até 37ºC.…”

Section: Resultsunclassified

Efeito da luz ultravioleta na eclodibilidade e viabilidade de pintos provenientes de ovos férteis sujos

Pereira¹,

Sousa²,

Rama³

et al. 2018

Revista Brasileira De Ciência Veterinária

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“…A comparison between composition of serum and serum proteins of different vertebrates conspicuously indicated that, at identical pH, ionic strength, and dilution, the ionic mobility of chick serum is highest in comparison to other vertebrates. The reason behind high mobility is the presence of high-mobility group proteins isolated from estrogenstimulated chick oviduct [25] (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Effect of Ampicillin and Chloramphenicol on Chick Serum

Nanda

Suneetha

2017

Asian J Pharm Clin Res

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Objective: Cystatin protein present in chick serum exhibits antimicrobial activity. The present study focuses on the effect of ampicillin and chloramphenicol antibiotics on chick serum and thus verifies Beer-Lambert's law. Methods:The serum is separated from the cellular matter with the help of a micropipette to get a clean serum sample. The quantification of protein was done by Lowry's method. The antibiotics ampicillin and chloramphenicol stock solution were prepared by 10 mg of antibiotic powder in 10 ml of sterilized water. The statistical analysis of the values obtained was done by SPSS logistics software. Results:The different values of concentration of serum with absorption showed a linear relationship which verifies Beer-Lambert's law. With an increase in the concentration of protein in chick serum, the absorption also increases, which gives a range of concentration of protein at which ampicillin and chloramphenicol act. Conclusion:The rise and fall in the absorbance rate of proteins after addition of different antibiotics represent the increase and decrease in the concentration of proteins, respectively. This shows that every antibiotic acts at a particular concentration on the protein of the serum. Therefore, proper doses of antibiotics are recommended by the doctors.

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Comparing the effectiveness of egg disinfectants against bacteria and mitotic indices of developing chick embryos

Cited by 33 publications

References 38 publications

Eggshell microbiology and quality of hatching eggs subjected to different sanitizing procedures

Eggshell microbiology and quality of hatching eggs subjected to different sanitizing procedures

Efeito da luz ultravioleta na eclodibilidade e viabilidade de pintos provenientes de ovos férteis sujos

Effect of Ampicillin and Chloramphenicol on Chick Serum

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