2023
DOI: 10.1093/femsyr/foad039
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Comparing the hierarchy of inter- and intra-species interactions with population dynamics of wine yeast cocultures

Eléonore Pourcelot,
Cleo Conacher,
Thérèse Marlin
et al.

Abstract: In winemaking, the development of new fermentation strategies, such as the use of mixed starter cultures with Saccharomyces cerevisiae (Sc) yeast and non-Saccharomyces (NS) species, requires a better understanding of how yeasts interact, especially at the beginning of fermentation. Despite the growing knowledge on interactions between Sc and NS, few data are available on the interactions between different species of NS. It is furthermore still unclear whether interactions are primarily driven by generic differ… Show more

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Cited by 4 publications
(8 citation statements)
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“…Similarly, ENO2 is also expressed throughout S. cerevisiae fermentation, even though to a lesser extent (Puig & Pérez-Ortín, 2000; Peng et al, 2015). Yeast transformation was carried out by electroporation, as described by Pourcelot et al (2023). Transformants were selected on YPD agar supplemented with the appropriate antibiotic: 800 µg/mL of hygromycin B for S. bacillaris , 100 µg/mL of nourseothricin for S. cerevisiae , T. delbrueckii , and L. thermotolerans , and 200 µg/mL of geneticin for H. uvarum and S. cerevisiae .…”
Section: Methodsmentioning
confidence: 99%
“…Similarly, ENO2 is also expressed throughout S. cerevisiae fermentation, even though to a lesser extent (Puig & Pérez-Ortín, 2000; Peng et al, 2015). Yeast transformation was carried out by electroporation, as described by Pourcelot et al (2023). Transformants were selected on YPD agar supplemented with the appropriate antibiotic: 800 µg/mL of hygromycin B for S. bacillaris , 100 µg/mL of nourseothricin for S. cerevisiae , T. delbrueckii , and L. thermotolerans , and 200 µg/mL of geneticin for H. uvarum and S. cerevisiae .…”
Section: Methodsmentioning
confidence: 99%
“…The strains used for S. cerevisiae, L. thermotolerans, T. delbrueckii, and S. bacillaris were each uniquely fluorescently tagged with an integrated fluorescent protein gene. This was done previously as in Pourcelot et al 2023. Two other microbes of interest were also included: the spoilage yeast Brettanomyces bruxellensis and the lactic acid bacteria Lactiplantibacillus plantarum. L. plantarum and B. bruxellensis strains were not fluorescently tagged.…”
Section: Strains and Fluorescent Markingmentioning
confidence: 99%
“…Numeration of live cells (i.e., viability) was assessed after staining cells with 1 μg/mL propidium iodide (PI, stored at 4°C protected from light; Calbiochem). Each population was previously tagged with one or two fluorescent proteins (Pourcelot et al 2023) and then detected with a specific set of channels described in Table X. Gating was done with the AttuneNxT software.…”
Section: Strains and Fluorescent Markingmentioning
confidence: 99%
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