Comparing the ultrastructural effects of two different cardiac preparation- and perfusion-techniques in a porcine model of extracorporal long-term preservation

Aupperle, H.; Garbade, Jens; Ullmann, C.; Schneider, Katja; Krautz, Christian; Dhein, Stefan; Gummert, Jan; Schoon, Heinz-Adolf

doi:10.1016/j.ejcts.2006.11.029

Cited by 19 publications

(10 citation statements)

References 15 publications

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“…17 In addition, preserving the donor heart in a beating state ex vivo can safely prolong the preservation period. 34 The somatic dimensions of several species of animals and of a wide variety of trees fit this rule well. It is a simple matter to show that energy metabolism during maximum sustained work depends on body cross-sectional area, not total body surface area.…”

Section: Discussionmentioning

confidence: 77%

A luminance-based heart chip assay for assessing the efficacy of graft preservation solutions in heart transplantation in rats

et al. 2013

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ObjectiveWe developed a novel luciferase-based viability assay for assessing the viability of hearts preserved in different solutions. We examined whether this in vitro system could predict heart damage and survival after transplantation in rats.DesignBy our novel system, preserved heart viability evaluation and transplanted heart-graft functional research study.SettingUniversity basic science laboratory.InterventionsIsolated Luciferase-transgenic Lewis (LEW) rat cardiac-tissue-chips were plated on 96-well tissue-culture plates and incubated in preservation solutions at 4°C. Viability was measured as photon intensity by using a bio-imaging system. Heart-grafts preserved in University of Wisconsin (UW), extracellular-trehalose-Kyoto (ETK), Euro-Collins (EC), histidin-tryptophan-ketoglutarat solution (HTK), lactated Ringer's (LR) or normal saline solution were transplanted cervically by using a cuff-technique or into the abdomens of syngeneic wild-type LEW rats by using conventional microsurgical suture techniques.Main outcome measuresImaging an evaluation of preservation heart-graft and functional analysis.ResultsCardiac-tissue-chips preserved with UW, HTK or ETK solution gave higher luminance than those preserved with EC, LR or normal saline (p<0.03). After 24 h of preservation of hearts in each solution at 4°C, the beating of the isolated hearts was evaluated. The success rate, evaluation of beating, of cervical heart transplants using UW and ETK solution exceeded 70%, but those using other preservation solutions were lower (UW: 100%, ETK: 75%, EC: 42.86%, HTK: 14.29%, normal saline: 0%). Histological analysis of cervical heart-grafts after 3 h preservation by myeloperoxidase (MPO), zona occludens-1(ZO-1), and caspase-3 immunostaining revealed different degrees of preservation damage in all grafts.ConclusionsOur novel assay system is simple and can test multiple solutions. It should therefore be a powerful tool for developing and improving new heart-graft preservation solutions.

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Section: Discussionmentioning

confidence: 77%

A luminance-based heart chip assay for assessing the efficacy of graft preservation solutions in heart transplantation in rats

et al. 2013

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“…This preparation was refined by using whole blood as a perfusate and by either perfusing the coronary arteries directly or filling the atria and chambers in a working heart model in pigs [11,30]. Previous work has been performed in hearts from the abattoir or in surgically explanted hearts [31].…”

Section: Discussionmentioning

confidence: 99%

Eprosartan improves cardiac function in swine working heart model of ischemia-reperfusion injury

Weymann

Sabashnikov

Patil

et al. 2014

Med Sci Monit Basic Res

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BackgroundEprosartan is an angiotensin II receptor antagonist used as an antihypertensive. We sought to evaluate the regional effect of Eprosartan on postinfarct ventricular remodeling and myocardial function in an isolated swine working heart model of ischemia-reperfusion injury.Material/Methods22 swine hearts were perfused with the Langendorff perfusion apparatus under standard experimental conditions. Myocardial ischemia was induced by a 10-min left anterior descending artery ligation. Hearts were reperfused with either saline (control group, n=11), or Eprosartan (treatment group, n=11). Left ventricular pressure (LVP) and regional heart parameters such as intramyocardial pressure (IMP), wall thickening rate (WTh), and pressure-length-loops (PLL) were measured at baseline and after 30 min of reperfusion.ResultsMeasured parameters were statistically similar between the 2 groups at baseline. The administration of Eprosartan led to a significantly better recovery of IMP and WTh: 44.4±2.5 mmHg vs. 51.2±3.3 mmHg, p<0.001 and 3.8±0.4 μm vs. 4.4±0.3 μm, p=0.001, respectively. PLL were also significantly higher in the treatment group following reperfusion (21694±3259 units vs. 31267±3429 units, p<0.01). There was no difference in the LVP response to Eprosartan versus controls (63.6±3.0 mmHg vs. 62.5±3.1 mmHg, p=0.400).ConclusionsPre-treatment with Eprosartan is associated with a significant improvement in regional cardiac function under ischemic conditions. Pharmacological treatment with eprosartan may exert a direct cardioprotective effect on ischemic myocardium.

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“…Aupperle et al. [21] reported using lower perfusion pressure (40–50 mmHg) that resulted in a better preservation of the ultrastructure in explanted hearts connected to the Langendorff system. Jones et al.…”

Section: Discussionmentioning

confidence: 99%

Continuous perfusion of donor hearts with oxygenated blood cardioplegia improves graft function

Zhang

Wen

et al. 2010

Transplant International

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Summary Donor hearts cannot be preserved beyond 6 h using cold storage (CS). Improving preservation methods may permit prolonged storage of donor heart. We compared graft function in large animal model after prolonged preservation (8 h) using continuous perfusion (CP) and CS method. Twenty‐four miniature pigs were used as donors and recipients. Donor hearts were either stored in University of Wisconsin solution (UW solution) for 8 h at 0–4 °C (CS group, n = 6) or were continuously perfused with oxygenated blood cardioplegia at 26 °C for 8 h (CP group, n = 6). After preservation, hearts were transplanted into recipients and reperfused for 3 h. Left ventricular (LV) function, cardiac output (CO), malondialdehyde (MDA) and adenosine triphosphate (ATP) levels, and water content were measured. Although water content of CP hearts was higher than that of CS, LV contractility and diastolic function of CP hearts were superior to those of CS. In addition, CP hearts performed better than CS hearts on CO in working heart state. ATP was better preserved and MDA levels were lower in CP hearts compared with those of CS (P < 0.0001). Donor hearts can be preserved longer using continuous perfusion with oxygenated blood cardioplegia and this method prevents time‐dependent ischemic injury.

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Comparing the ultrastructural effects of two different cardiac preparation- and perfusion-techniques in a porcine model of extracorporal long-term preservation

Abstract: Avoiding cardioplegia and hypothermia, and using lower perfusion pressure resulted in a better preservation of the ultrastructure in explanted hearts at the Langendorff system.

Cited by 19 publications

References 15 publications

A luminance-based heart chip assay for assessing the efficacy of graft preservation solutions in heart transplantation in rats

A luminance-based heart chip assay for assessing the efficacy of graft preservation solutions in heart transplantation in rats

Eprosartan improves cardiac function in swine working heart model of ischemia-reperfusion injury

Continuous perfusion of donor hearts with oxygenated blood cardioplegia improves graft function

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