Grapes (Vitis vinifera) is one of the important commercial subtropical crops, which is a good source of vitamins and minerals. It is grown throughout the world except the places with extreme temperature and high altitude .So, to find out Effect of PGR on growth and establishment of grapes cv. Thompson Seedless under Prayagraj agro climatic condition; a field experiment was conducted at Horticulture Research Farm, Department of Horticulture, Naini Agriculture Institute, Sam higginbottom University of Agriculture, Technology and Science, Prayagraj, U.P-211007 during the year 2022. The experiment comprised of 10 different treatment of three different plant growth regulator comprising of GA3, IAA and IBA with different concentration of 50 ppm, 100 ppm and 150 ppm; 100 ppm, 300 ppm and 500 ppm; 1000 ppm, 2000 ppm and 3000 ppm respectively. The main objective of the experiment was to evaluate the root and vegetative growth of grapevines with their success and survival in the Prayagraj agro-climatic zone. From the present investigation, treatment T9 (IBA @3000 ppm) was found best with least days to emerge from the bud from the grapes cutting (6.4 days) with maximum amount of sprouting percentage (77.7%). It also showed the best root growth parameters with maximum root length 25.1cm @120DAP and maximum number of roots per cutting 49.3 @120DAP. It also showed best vegetative growth parameters with maximum shoot length 13.4cm @120DAP, maximum stem diameter 28.1mm @120DAP, highest number of leaves with 12.5 @120 DAP, maximum leaf area 73.9cm2, maximum plant height after transplanting 14.9cm and highest survival percentage 88.8% of grapes plant. The T9 showed the best results with all major parameters when cuttings were treated with different concentrations of plant growth regulators.