Comparision of Piceid and Resveratrol in Antioxidation and Antiproliferation Activities In Vitro

Abstract

BackgroundThe clinic therapeutic effect of resveratrol is limited due to its low oral bioavailability. Piceid, a precursor of resveratrol, is the most abundant form of resveratrol in nature. A number of studies have hypothesized that piceid may have the same bioactivities like those of resveratrol. The aim of this work is to compare piceid with resveratrol in antioxidation and antiproliferation activities in vitro.MethodsThe antioxidative effects of resveratrol and piceid were evaluated by phenanthroline-Fe2+ method and H2O2-induced oxidative injury cell model. The antiproliferation effects were determined by MTT method in human liver tumor HepG2 cells, human breast cancer MDA-MB-231 cells and MCF-7 cells. The effects of resveratrol and piceid on the cell cycle and the apoptosis were evaluated by flow cytometry. Additionally, the uptake profiles of resveratrol and piceid in cancer cells were observed using fluorescence microscopy and clarified by LC-MS/MS.ConclusionPiceid exhibited higher scavenging activity against hydroxyl radicals than resveratrol in vitro. Resveratrol showed a significant protective effect against H2O2-induced cell damage. What is more, resveratrol had biphasic effects on tumor cells. Resveratrol and piceid only showed significant cytotoxicity on tumor cells at high concentration (≥50 µmol/L), while low concentration of resveratrol (<30 µmol/L) increased the cell viability. The principal effect of resveratrol and piceid on the viability of tumor cells was caused by the cell cycle arrest, while the effect on apoptosis was relatively minor. The reason that piceid showed lower biological activity than resveratrol at the same concentration was probably because piceid was more difficult in being uptaken by cells.

Cancer prevention and therapy through the modulation of the tumor microenvironment

“…Resveratrol has low toxicity [281]. Various studies report anti-cancer effects [282–284], including the suppression of metastasis [285], and induction of proliferative arrest [286]. …”

Cytotoxic, Antiangiogenic and Antitelomerase Activity of Glucosyl‐ and Acyl‐ Resveratrol Prodrugs and Resveratrol Sulfate Metabolites

“…In addition, one normal cell line, the human embryonic kidney cell line (HEK-293) was employed in the assays for comparison (29). The standard MTT assay was used as described in the When piceid is compared with resveratrol, IC50 values were in the same range or higher (31,33). In our case, piceid IC50 values measured were in between those reported previously and in both cell lines (HT-29 and MCF-7) were lower than those obtained for resveratrol.…”

An Automated Multidose Synthesis of the Potentiometric PET Probe 4-[18F]Fluorobenzyl-Triphenylphosphonium ([18F]FBnTP)

“…Analysis of the activity that had passed through the Accell CM revealed a radiosignal of an unidentified, nonionic radiolysis product that accounted for up to 87 % of the waste activity and was not observed in low-activity runs. Addition of radical scavengers such as Na-ascorbate, ascorbic acid, and N -tert-butyl-α-phenylnitrone (PBN) [22] or reservatrol-3-β-mono- d -glucoside (polydatin) [26] either prevented trapping of [ 18 F]FBnTP on the Accell CM or did not hinder radiolysis. Trapping efficiencies were low in pure EtOH and water is known to facilitate radiolysis [22].…”