The study aimed to investigate the enhancement of biogas production through anaerobic digestion from blends of wastewater and microalgae. The microalgae functioned as a co-substrate. A series of laboratory-scale batch anaerobic co-digestion of the wastewater and microalgae were carried out under mesophilic conditions for 21 days. Biogas production rates from wastewater (WW) alone and different blends of WW and microalgae (MA) were analysed. In addition, the nutrient values and reduction in the volume of the WW after digestion were determined. The results show that the quantity and quality of biogas produced with different mix ratios of WW to MA (WW only, 3 : 1, and 3 : 2) were 24 mL CH4/g COD, 37 mL CH4/g COD, and 44 mL CH4/g COD, respectively. At the optimum mix ratio, the optimum methane produced was 44 mL CH4/g COD, which is much lower than volumes of gas STP conditions, which is 350 mL CH4/g COD. The values of TS, VS, and COD were also reduced by 43.11%, 40.09%, and 71.99% at the optimum mix ratio, respectively. The optimum mix ratio of 1732.77, 77.14, and 174.26 kg/year of urea, diammonium phosphate, and potash fertilizer, respectively, was obtained. The results indicate that biogas production can be improved through co-digestion of WW and MA as a co-substrate relatively, but to improve the production rate another substrate should be investigated. However, the result can be used as a supplementary investigation for practical application in energy production.