Among the most important trade bridges for Western Europe and the Balkans is the Port of Igoumenitsa in Western Greece, which experiences heavy traffic of goods and passengers. This case analysis primarily aims to investigate the effect of shipping and port operations on air quality in the Port of Igoumenitsa, comparing two representative short case seasons of air quality measurement campaigns. The high activity season ranges from 25 to 31 August 2018, while the low season ranges from 17 to 25 May 2018. A mobile air quality monitoring system was used to perform the analysis. To compare the air quality of the examined periods, the wind speed rose diagrams, the correlation analysis, as well as the hourly variations in concentrations of pollutants and meteorological parameters, were studied. In addition, the impact of meteorology and atmospheric circulation on local air quality were investigated. For study implementation, various types of data, obtained from NASA Worldview application, Barcelona Supercomputing Center (BSC), and Giovanni online system (NASA-GES-DISC) were included. The results indicate that port operations affect the air quality in the Port of Igoumenitsa, as the high season showed higher concentrations of air pollutants compared to the low season. A notable exception was the concentration of PMs, which was affected by an African dust transfer event during the low season. Finally, the findings indicate that climatic factors affect the pollution levels of the case analysis, and emphasize the importance of developing a green and sustainable management system within the port.