Comparison and characteristics of the formation of different adipose tissues in ducks during early growth

Kou, Jie; Wang, W. X.; Liu, H. H.; Pan, Zhenxiao; He, Tao; Hu, Jiwei; Li, L.; Wang, J. W.

doi:10.3382/ps.2012-02273

Cited by 9 publications

(4 citation statements)

References 43 publications

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“…However, like 39ºC, egg incubation at 36°C also reduced the size of neck adipocytes, but its effect was less pronounced because adipocytes were only approximately 30% smaller compared to 37.5°C. Since adipocyte hypertrophy is directly related to fat deposition ( Tchernof et al, 2006 ; Kou et al, 2012 ; Choe et al, 2016 ), this supports the idea that high incubation temperature induced a decrease in fat deposition in neck and abdominal adipocytes, while a low incubation temperature induced increased deposition in thigh and abdominal adipocytes and a decreased deposition in neck adipocytes. From a developmental view, our results are of great interest and importance because they reveal a close link between egg incubation temperature during fetal development and postnatal adiposity in broilers.…”

Section: Discussionsupporting

confidence: 55%

Long-Lasting Effects of Incubation Temperature During Fetal Development on Subcutaneous Adipose Tissue of Broilers

Almeida

Morita²,

Matos³

et al. 2022

Front. Physiol.

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Increasing evidence indicates that fetal programming may cause permanent effects on offspring adipose tissue and body composition. Previous study showed reduction in newly-hatched broiler chick adiposity by manipulating incubation temperature during fetal development. The present study examined whether incubation temperature during fetal development has long-term effects on post-hatching fat deposition in broilers. Broiler breeder eggs (Cobb-500®) were incubated under constant low (36°C, LT), control (37.5°C, CT) or high (39°C, HT) temperature from day 13 onward, giving to eggshell temperature of 37.3 ± 0.08°C, 37.8 ± 0.2°C, and 38.8 ± 0.3°C, respectively. Male chicks were reared under recommended temperatures until 42 days old. LT 21 days old broilers exhibited higher blood cholesterol than CT broilers, and higher triglycerids, VLDL, and LDL, and lower HDL than CT and HT broilers. LT broilers presented higher liver cholesterol than CT broilers and lower ether extract percentage than CT broilers. Adipocyte count was lower in the abdomen than in the thigh. Until day 21 of age, feed intake was higher in LT than in HT broilers. At day 42 of age, blood cholesterol and LDL were higher in HT broilers than in CT and LT broilers. Liver cholesterol was higher in LT than in HT broilers. LT treatment reduced neck and increased thigh adipocyte size compared to CT treatment, while the HT treatment reduced abdomen and neck adipocyte size compared to other two treatments and in the thigh compared to LT treatment. In CT broilers, thigh adipocytes were smaller than abdomen and neck adipocytes. HT treatment increased adipocyte number per area in the neck compared to LT and CT treatment, and LT and HT treatments reduced adipocyte count in the thigh compared to CT treatment. CT broilers presented higher adipocyte count in the thigh than the abdomen and neck, while HT broilers presented higher adipocyte count in the neck than the abdomen and thigh. Cell proliferation was lower in the abdomen than in the thigh. The results show incubation temperature manipulation during fetal development has long-term and distinct effects on regional adiposity, and can be used to modulate broiler fat deposition.

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Section: Discussionsupporting

confidence: 55%

Long-Lasting Effects of Incubation Temperature During Fetal Development on Subcutaneous Adipose Tissue of Broilers

Almeida

Morita²,

Matos³

et al. 2022

Front. Physiol.

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“…In contrast to broiler chickens, we found that the TG content in SAT and AAT showed a“rise, decline and rise” trend. The discrepancy may result from our use of samples from multiple regions of adipose tissue rather than from a single specific location, as described by Kou et al (2012) . As the TG content reflects the fat storage capacity, our findings suggest that AAT may display greater fat storage capacity than the SAT from P28 to P120.…”

Section: Discussionmentioning

confidence: 99%

Difference in developmental dynamics between subcutaneous and abdominal adipose tissues in goose (Anser Cygnoides)

Huo

Weng

et al. 2021

Poultry Science

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Goose ( Anas cygnoides ), as a typical species domesticated from a migratory bird, has maintained the capability of depositing excess lipid and preferentially accumulating fat within the abdomen and subcutaneous, which not only leads to decrease in yield of meat product, but also affects the feed conversion rate. Here, an experiment was conducted to examine the difference in developmental dynamics between subcutaneous ( SAT ) and abdominal adipose tissues ( AAT ) in goose. The results showed that SAT could be clearly observed at embryonic days (E) 15, whereas AAT were clearer until E20. Although the weights of SAT and AAT showed a significant rising with advancing age ( P < 0.05), their gains were not completely uniform, and more adipose deposited preferentially toward AAT after birth ( P < 0.05). Additionally, a clear expansion in adipocyte size was observed in AAT and SAT during embryonic stages ( P < 0.05). The average adipocyte area in AAT continued to increase after birth ( P < 0.05), while the cell areas in SAT were relatively invariable ( P > 0.05). Furthermore, the expression levels of FABP4/aP2, ACSL1 and PPARγ were much higher in SAT than in AAT, whereas relative higher expression level of IL-6 was observed in the AAT during embryonic stages. After birth, the more expression of LPL and PPARα were detected in AAT than did in SAT ( P < 0.05), whereas greater ATGL expression was in SAT ( P < 0.05). Taken together, these findings suggest that AAT may display greater fat storage capacity than SAT accompanied by changes in cell area and lipogenic capacity. Considering that there is disparity in the individual adipose tissues, we suggested that careful consideration for the precise interventions used to control SAT or AAT deposition in meat-producing animals to improve feed efficiency.

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“…Peking duck (Anas platyrhynchos) is a world famous waterfowl breed that is able to accumulate large quantities of lipid in the subcutaneous adipose tissue (Attia et al 2012;Ding et al 2012). It has been well established that the development of duck adipose tissue is due to a combination of adipocyte hyperplasia and hypertrophy from hatching until 4 weeks of age, after which adipose expansion is accomplished exclusively by adipocyte hypertrophy (Kou et al 2012). However, the molecular mechanism involving the growth and differentiation of duck preadipocytes remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Isolation, culture and differentiation of duck (Anas platyrhynchos) preadipocytes

Ding

et al. 2014

Cytotechnology

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In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate into mature adipocytes by the addition of a cocktail containing dexamethasone, insulin, 3-isobutyl-1-methylxanthine, and oleic acid for 8 days. Successful differentiation was demonstrated by the development of lipid droplets and the expression of key marker genes including peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding protein-α (CEBP/α) and adipocyte fatty acid-binding protein (FABP4). Our results showed that duck primary preadipocytes began to adhere 12 h after seeding as short spindle shapes or litter triangles, which grew quickly 3 days post attachment and maintained stable after day 7. After 8 days the preadipocytes were induced to differentiate into mature adipocytes, which were stained red by oil red O. Additionally, it showed that during preadipocyte differentiation PPARγ mRNA was highly expressed at day 3, while CEBP/α and FABP4 mRNA peaked at day 5 and 8, respectively. These results indicate that we have successfully isolated and cultured Peking duck preadipocytes and successfully induced them to differentiate into mature adipocytes. This work could lay a foundation for further research into waterfowl adipogenesis.

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Comparison and characteristics of the formation of different adipose tissues in ducks during early growth

Cited by 9 publications

References 43 publications

Long-Lasting Effects of Incubation Temperature During Fetal Development on Subcutaneous Adipose Tissue of Broilers

Long-Lasting Effects of Incubation Temperature During Fetal Development on Subcutaneous Adipose Tissue of Broilers

Difference in developmental dynamics between subcutaneous and abdominal adipose tissues in goose (Anser Cygnoides)

Isolation, culture and differentiation of duck (Anas platyrhynchos) preadipocytes

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