Comparison and Characterization of Compounds with Antioxidant Activity in Lycium barbarum Using High‐Performance Thin Layer Chromatography Coupled with DPPH Bioautography and Tandem Mass Spectrometry

Abstract

Methanol extracts from 50 batches of Lycium barbarum (L. barbarum, wolfberry) in China were compared and characterized using high-performance thin-layer chromatography coupled with 2,2-diphenyl-1-picrylhydrazyl (DPPH) bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS), respectively. Results showed that similar components occupying the major antioxidant activity existed in L. barbarum collected from different origins. However, the average antioxidant capacities of methanol extracts of L. barbarum collected in Ningxia were significantly higher than those of Qinghai, Xinjiang, Inner Mongolia, and Gansu, which may contribute to rational use of L. barbarum in China. Furthermore, the chemical structure of compound with the highest antioxidant capacity was tentatively identified as 2-O-β-d-glucopyranosyl-l-ascorbic acid using ESI-Q-TOF-MS/MS analysis, which possessed high potentials to be used as an antioxidant biomarker for the quality control of L. barbarum. Results are helpful for the bioactivity-based quality control of L. barbarum, and beneficial for the improvement of their performance in functional/health foods area, suggesting that HPTLC-DPPH bioautography with ESI-Q-TOF-MS/MS could be used as a routine approach for quality control of antioxidant components in L. barbarum.

Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health

“…In bow milk, know to have very low antioxidant properties and so used as negative control [ 18 ], TCP value was 17 ± 1 mgGAE/100gr and ORAC value was 855 ± 2 μmolTE/100gr DW. In LB berry extratcs, know to have very high antioxidant properties and so used as positive control [ 19 ], TCP value was 943 ± 231 mgGAE/100gr and ORAC value was 26502 ± 3807 μmolTE/100gr DW (Fig. 1 ).…”

Rapid Identification and Comparison of Compounds with Antioxidant Activity in Coreopsis tinctoria Herbal Tea by High‐Performance Thin‐Layer Chromatography Coupled with DPPH Bioautography and Densitometry

“…The detection wavelength for densitometry scanning was chosen based on the overlaid absorption spectra of antioxidant bands. The overlaid spectra revealed that the maximum absorption wavelength for antioxidant bands was 535 nm, and 535 nm was selected for detection (Lam and others ).…”

“…HPTLC not only greatly reduces analysis time and consumption of organic solvents, but also provides adequate resolutions for the isolation of compounds with antioxidant activity because of the smaller particle size of stationary phase compared to general TLC plate (Tistaert and others ). Antioxidants isolated by HPTLC after postderivatization of DPPH solutions were displayed in the form of yellowish fluorescent bands with purple background, which were easy to be identified and were of high sensitivity (Jesionek and others ; Lam and others ). Overall, HPTLC‐DPPH bioautography can serve as an efficient approach to isolate compounds with antioxidant activity and to identify multiple samples in parallel.…”

Effect of composition and processing conditions on selected characteristics of extruded corn instant gruels enriched with fruits addition

“…Goji berries are characterized red-orange colour formed by carotenoids [23]. One of the most important components of goji berries are polyphenols with high antioxidant activity [24]. The biggest group of components are compexed polysaccharides, and moreover vitamins as ascorbic acid, tiamin, ryboflavin or glucopyranosids.…”