Relevance. Currently, growth regulators are gaining increasing popularity and are in great demand both among amateur gardeners and among scientists and researchers; this is due to the ir ability to stimulate and regulate the growth and development of various crops. Currently, the use of cultivar stimulants in nursery practice has been studied variants with non-root treatments in the callusing room at nursery garden and when soaking the basal part of the cuttings immediately before planting. There is no study of the use of growth stimulants on the components of grafting immediately before its implementation in the literature, which makes our research desirable and relevant. The aim of the study is to improve the elements of the technology of production of grafted grape seedlings by soaking the components of grafting in growth-stimulating preparations to improve the quality and yield of grafted grape seedlings from the callusing room. Methods and results. Methods of research – generally accepted in the practice of grape nursery (Kolesnik L. V., Melnik S. A., Shchiglovskaya V. I.). It was found that the greatest regenerative activity for the Tsimlyansky Cherniy variety was shown in the variant with soaking of the rootstock component of the grafting in the “NanoCremniy” preparation, in which 96.7% of plants had budding on the 15th day of callusing and 95.9 % of grafting seedlings had a circular callus. On the Cabernet Sauvignon variety, a variant with soaking of the graft part in the “NanoCremniy” preparation was distinguished (93.3 % and 92.5 % respectively). The most complete stimulation of growth processes after planting is reflected in the variants with the use on the Tsimlyansky Cherniy variety preparations “NanoCremniy” (80.0 %) and “Cultimar” (88.3 %).