Comparison between adsorption and covalent coupling of proteins to solid phases using different polymers as support

Immobilisation of Antibodies and Antigens on Macro Solid Phases — A Comparison Between Adsorptive and Covalent Binding. A critical study of macro solid phases for use in immunoassay systems, Part I

“…Adsorption of antibodies and antigens onto ion-exchangers is no alternative, äs such electrostatic binding is highly susceptible to pH and salt-concentration changes, resulting in a high unspecific binding in addition to the loss in sensitivity and precision mentioned above (5) Covalent coupling of molecules to microparticles such äs cellulose, glass of nylon leads to an increase in stability, but does not alleviate the need for centrifugation. In order to improve the ease of handling in assays alfeady published from this Department (6)(7)(8), a prelirriinäry study of solid-phases was carried out to test their suitability for immobilisation of reaction partners (4,9). The results here presented enlarge üpoft this study, and present in detail the methods of immobilising molecules on macro-solid-phases, here balls of 6-7 mm diameter made of glass, polystyrene or nylon.…”