Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression

Khodavandi, Alireza; Harmal, Nabil S.; Alizadeh, Fahimeh; Scully, Olivia Jane; Sidik, Shiran Mohd; Othman, Fauziah; Sekawi, Zamberi; Ng, Kee Peng; Chong, Pei Pei

doi:10.1016/j.phymed.2011.08.060

Cited by 56 publications

(38 citation statements)

References 36 publications

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“…Candida is a genus of yeasts and replicate quickly, therefore are easy to manipulate genetically to make them produce more desired substances for synthesis. C. albicans is harmless commensals or endosymbiosis of hosts including humans; however, when mucosal barriers are disrupted or the immune system is compromised they can invade and cause disease [14]. Moreover, Di Giacomo et al [15] used growing cells of C. albicans in combination with carbon nanotubes to produce stable electrically conductive bio-nanocomposite tissue materials that have been used as temperature sensing elements.…”

Section: Discussionmentioning

confidence: 99%

Mycosynthesis of Silver Nanoparticles from Candida albicans and its Antibacterial Activity against Escherichia coli and Staphylococcus aureus

Rahimi¹,

Alizadeh²,

Khodavandi³

2016

Trop. J. Pharm Res

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Section: Discussionmentioning

confidence: 99%

Mycosynthesis of Silver Nanoparticles from Candida albicans and its Antibacterial Activity against Escherichia coli and Staphylococcus aureus

Rahimi¹,

Alizadeh²,

Khodavandi³

2016

Trop. J. Pharm Res

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“…Once exposed to the atmospheric air, allicin is converted into diallydissulphinate, which has antimicrobial activity. 10,11 …”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Two Garlic Species (Allium Sativum and A. Tuberosum) Against Staphylococci Infection. In Vivo Study in Rats

Pc¹,

et al. 2017

Adv Pharm Bull

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Purpose: This study observed the effect of garlic extracts and amoxicillin against an induced staphylococcal infection model. MIC and MBC were also obtained for aqueous extracts of Allium sativum (Asa) and Allium tuberosum (Atu) against Staphylococcus aureus penicillin-sensitive (PSSA - ATCC 25923) and MRSA (ATCC 33592). Methods: Granulation tissues were induced in the back of 205 rats. After 14 days, 0.5 mL of 108 CFU/mL of PSSA or MRSA were injected inside tissues. After 24h, animals were divided: G1 (Control) – 0.5 mL of NaCl 0.9%; G2 – Asa 100 mg/kg or 400mg/kg; G3 – Atu 100 mg/kg or 400 mg/kg; G4 – amoxicillin suspension 50 mg/kg, considering PSSA infection; and G5 (Control) - 0.5 mL of NaCl 0.9%; G6 – Asa 400mg/kg; G7 – amoxicillin 50 mg/kg; and G8 - Asa 400 mg/kg + amoxicillin 50 mg/kg for MRSA. All treatments were administered P.O. every 6h. Animals were killed at 0, 6, 12 and 24h. Samples were spread on salt-mannitol agar. Colonies were counted after 18 h at 37 °C. Atu was not able to inhibit or kill PSSA and MRSA. Considering Asa, MIC and MBC against PSSA were 2 mg/mL and 4 mg/mL, respectively; and 16 mg/mL and 64 mg/mL against MRSA. Results: No effect was observed in vivo for control, Asa 100 mg/kg and Atu 100 mg/kg, while amoxicillin, Atu 400 mg/kg and Asa 400 mg/kg decreased PSSA counts in all-time points. No effect of any group against MRSA was observed at any time. Conclusion: Thus, A. sativum and A. tuberosum were able to reduce PSSA infection, but not MRSA infection.

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“…Several chemicals from traditional herbs, such as tetrandrine (Zhang et al 2013) and allicin (Khodavandi et al 2011), have been recognised as alternatives to enhance the antifungal activity of FLC. Flavonoids are a group of chemicals, and have also been reported to have broad-spectrum antibacterial and antifungal activities (Friedman 2007;Orhan et al 2010;Peralta et al 2012).…”

Section: Introductionmentioning

confidence: 99%

The roles of CDR1, CDR2, and MDR1 in kaempferol-induced suppression with fluconazole-resistant Candida albicans

Shao

Zhang

Wang

et al. 2015

Pharmaceutical Biology

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Context: Fungal infections caused by fluconazole-resistant Candida albicans are an intractable clinical problem, calling for new efficient antifungal drugs. Kaempferol, an active flavonoid, has been considered a potential candidate against Candida species. Objective: This work investigates the resistance reversion of kaempferol in fluconazole-resistant C. albicans and the underlying mechanism. Materials and methods: The antifungal activities of fluconazole and/or kaempferol were assessed by a series of standard procedures including broth microdilution method, checkerboard assay and time-kill (T-K) test in nine clinical strains as well as a standard reference isolate of C. albicans. Subsequently, the morphological changes, the efflux of rhodamine 6G, and the expressions of CDR 1, CDR 2, and MDR 1 were analysed by scanning electron microscope (SEM), inverted fluorescence microscope and quantitative reverse transcription polymerase chain reaction (qRT-PCR) in C. albicans z2003.Results: For all the tested C. albicans strains, the minimum inhibitory concentrations (MICs) of fluconazole and kaempferol ranged 0.25-32 and 128-256 mg/mL with a range of fractional inhibitory concentration index of 0.257-0.531. In C. albicans z2003, the expression of both CDR 1 and CDR 2 were decreased after exposure to kaempferol alone with negligible rhodamine 6G accumulation, while the expression of CDR 1, CDR 2 and MDR 1 were all decreased when fluconazole and kaempferol were used concomitantly with notable fluorescence of rhodamine 6G observed. Discussion and conclusion: Kaempferol-induced reversion in fluconazole-resistant C. albicans might be likely due to the suppression of the expression of CDR1, CDR2 and MDR1.ARTICLE HISTORY

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Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression

Cited by 56 publications

References 36 publications

Mycosynthesis of Silver Nanoparticles from <i>Candida albicans</i> and its Antibacterial Activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>

Mycosynthesis of Silver Nanoparticles from <i>Candida albicans</i> and its Antibacterial Activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>

Antimicrobial Activity of Two Garlic Species (Allium Sativum and A. Tuberosum) Against Staphylococci Infection. In Vivo Study in Rats

The roles of CDR1, CDR2, and MDR1 in kaempferol-induced suppression with fluconazole-resistant Candida albicans

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