Comparison between Enterotoxigenic <i>Escherichia coli</i> Strains Expressing “F42,” F41 and K99 Colonization Factors

Sperandio, Vanessa; Silveira, Wanderley Dias da

doi:10.1111/j.1348-0421.1993.tb01718.x

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…These are sometimes present in porcine E coli strains but were not examined in the present study. 8,[29][30][31][32]35,36 Another possibility to consider is that these isolates could possess the eae gene, which would classify them as attaching and effacing E coli (AEEC). AEEC are occasionally associated with ND and PWD in pigs and do not characteristically produce toxins, but instead cause effacement and rearrangement of the enterocyte cytoskeleton, resulting in malabsorptive diarrhoea.…”

Section: Discussionmentioning

confidence: 99%

Rapid identification of virulence genes in enterotoxigenic Escherichia coli isolates associated with diarrhoea in Queensland piggeries

T¹,

Stephens²,

Townsend³

et al. 2005

Aust Veterinary J

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Multiplex PCR was a rapid and specific method for detecting the presence of ETEC virulence genes in porcine E coli isolates. For isolates obtained from cases of suspected colibacillosis in Queensland, growth of a heavy pure culture of haemolytic E coli was a sensitive prognostic indicator of the presence of ETEC virulence genes in the isolate. ETEC pathotypes and serotypes remained stable in Queensland piggeries over the five-year study period and appear to have changed little over the last three decades.

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Section: Discussionmentioning

confidence: 99%

Rapid identification of virulence genes in enterotoxigenic Escherichia coli isolates associated with diarrhoea in Queensland piggeries

T¹,

Stephens²,

Townsend³

et al. 2005

Aust Veterinary J

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“…The Electronic Microscopy was carried out as described by Sperandio and Silveira [39]. For this purpose, the bacterial strain was grown in LB medium at 37℃, overnight.…”

Section: Methodsmentioning

confidence: 99%

The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain

et al. 2008

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An Escherichia coli strain (SEPT13) isolated from the liver of a hen presenting clinical signs of septicaemia had a LD50 of 4.0 × 105 CFU/ml in one-day-old chickens, expressed Ia, Ib, E1, E3, K and B colicins and aerobactin. The strain was ampicillin and streptomycin resistant, and found to have fimA, csgA and tsh DNA related sequences; it could adhere to and invade HEp-2 and tracheal epithelial cells, expressed fimbriae (observed by electron microscopy), and had five plasmids of 2.7, 4.7, 43, 56, and 88 MDa. Transposon mutagenesis of strain SEPT13, with transposon TnphoA, resulted in a mutant strain named ST16 that had a LD50 of 1.2 × 1012 CFU/ml. All other biological characteristics of strain ST16 were the same as those detected for strain SEPT13 except for the migration of an 88 MDa plasmid to the 93 MDa position indicating the insertion of the transposon into the 88 MDa plasmid. The 93 MDa plasmid of strain ST16 was transferred, by electroporation assay, to non-pathogenic receptor strains (E. coli strains K12 MS101 and HB101), resulting in transformant strains A and B, respectively. These strains exhibited adhesion properties to in vitro cultivated HEp-2 cells but did not have the capacity for invasion. The adherence occurred despite the absence of fimbriae; this finding suggests that the 88 MDa plasmid has afimbrial adhesin genes.

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“…E. coli strains of enterotoxic colibacillosis in suckling piglets are characterized by one or the other of the K88 adhesins (in variants K88ab, K88ac, and K88ad) also known as the (F4), by K99 (F5) or 987P (F6) adhesins and occasionally by the F41 , F165 (Fairbrother et al, 1986) or F42 adhesins (Sperandio and da Silveira, 1993). Among these adhesins K88 (F4) and 987P (F6) are specific for pigs, while K99 (F5) and F41 seem to have receptors in both pigs and calves.…”

Section: Adhesins and Receptors For Etec Of Pigsmentioning

confidence: 99%

Preface

Holzapfel¹,

Naughton²

2005

Microbial Ecology in Growing Animals

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Enteric diseases of pigs and calves owing to Escherichia coli typically appear during the first few days (and weeks) of life. The so far recognized pathotypes of E. coli involved are the enterotoxic E. coli (ETEC), verotoxic E. coli (VTEC), enteropathogenic E. coli (EPEC), and necrotoxic E. coli (NTEC). The first step in the pathogenesis of all these types is to adhere to the intestinal microvilli with or without inducing morphological lesions and produce specific toxins acting locally on enterocytes and/or absorbed into the bloodstream. This action is assured by specific ligand (adhesins) and receptor interactions which are characteristic of the pathotypes involved and may vary according to the animal species. The host-adapted adhesin/receptor systems are targets of several preventive measures including vaccines, receptor blocking and breeding for genetic resistance. They also provide the basis for cross-species infections including zoonoses. Therefore they deserve the attention of epidemiologists, research scientists and technologists. Besides, they provide tools for increased understanding of molecular pathogenesis, and offer excellent models for comparative studies of different disease entities of enteric colibacillosis in humans.

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Comparison between Enterotoxigenic Escherichia coli Strains Expressing “F42,” F41 and K99 Colonization Factors

Cited by 7 publications

References 35 publications

Rapid identification of virulence genes in enterotoxigenic Escherichia coli isolates associated with diarrhoea in Queensland piggeries

Rapid identification of virulence genes in enterotoxigenic Escherichia coli isolates associated with diarrhoea in Queensland piggeries

The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain

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