1979
DOI: 10.1007/bf00344838
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Comparison between pressure-volume and dewpoint-hygrometry techniques for determining the water relations characteristics of grass and legume leaves

Abstract: The water relations characteristics of three grass species (Panicum maximum var. trichoglume, Cenchrus ciliaris, Heteropogon contortus), and a legume (Macroptilium atropurpureum) grown in the field were measured using both a modified pressure/volume technique with pressure bomb measurements on single leaves and a dewpoint hygrometry technique applied to fresh and to frozen and thawed leaf discs.The two techniques agreed well in the estimates of osmotic potential at full turgor and the water potential at zero t… Show more

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Cited by 200 publications
(84 citation statements)
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“…Leaf tissues were thawed and centrifuged at 1,200 X g for 25 min at 4"C to extract the cell sap. Osmotic potential (f,,) of lhe cell sap was measured using a Wescor model 5,500 vapor pressure osmometer (Wescor, Logan, UT), and osmotic potential at full turgor (f"(loo)) was calculated by adjusting for the relative water content (RWC) as described by Wilson et al (1979). RWC was measured as described by Saneoka et al (1996).…”
Section: Methodsmentioning
confidence: 99%
“…Leaf tissues were thawed and centrifuged at 1,200 X g for 25 min at 4"C to extract the cell sap. Osmotic potential (f,,) of lhe cell sap was measured using a Wescor model 5,500 vapor pressure osmometer (Wescor, Logan, UT), and osmotic potential at full turgor (f"(loo)) was calculated by adjusting for the relative water content (RWC) as described by Wilson et al (1979). RWC was measured as described by Saneoka et al (1996).…”
Section: Methodsmentioning
confidence: 99%
“…Osmotic potential at full p ( sfp ) and the apoplastic water content (RWCapo) were calculated by extrapolating the linear portion of the curve to the y-axis and to the x-axis, where sfp is the water potential at RWC = 0 and RWCapo is the RWC at 1/ = 0. The bulk modulus of elasticity ( ) at 100 % RWC was estimated by assuming an almost linear relationship between and RWC, using the methods of Wilson et al (1979). These data were obtained at the end of the drought period in all the N treatments, in wellwatered and water-stressed plants.…”
Section: Measurementsmentioning
confidence: 99%
“…Dry weight was determined on seedlings kept at 70 o C for 48 hours. All findings were analysed in accordance with Wilson et al [40]. As well as measuring initial water potential (Ψ) and actual percentage of water content (WC), the osmotic potential at full turgor (Ψ π100 ), the percentage of apoplastic water (B), the percentage of water deficit at incipient plasmolysis (WD 0 ) and the maximum elasticity module (ε) were also estimated.…”
Section: Pressure-volume Curvesmentioning
confidence: 99%