Comparison between proton-induced x-ray emission (PIXE) and total reflection x-ray fluorescence (TXRF) spectrometry for the elemental analysis of human amniotic fluid

Liendo, J.A.; Gonzalez, Andrew A.; Castelli, Cristina; Gómez, J.; Jiménez, J P Oswaldo; Marcó, L.; Sajó-Bohus, L.; Greaves, E.D.; Fletcher, N.R.; Bauman, S.

doi:10.1002/(sici)1097-4539(199901/02)28:1<3::aid-xrs298>3.0.co;2-5

Cited by 20 publications

(6 citation statements)

References 11 publications

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“…In addition to blood, other bodily fluids such as amniotic and cerebrospinal fluid can also be measured by TXRF. Amniotic fluids can be prepared either by digesting the sample with concentrated nitric acid or through simple centrifugation without further sample pretreatment [135]. Cerebrospinal fluid can be decomposed directly on the sample carrier by adding nitric acid to it [126], [136].…”

Section: Txrf Sample Preparationmentioning

confidence: 99%

Gold Nanoparticle Quantification With Total Reflection X-ray Fluoresence

Mankovskii

2024

Preprint

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<p>The quantification of gold nanoparticle (AuNP) uptake is of importance to a variety of therapeutic, diagnostic and theranostic applications. In particular, the accurate assessment of AuNP delivery remains challenging as current approaches employ cumbersome sample preparation methods. In this work, an easy-to-use and accurate Total reflection X-Ray Fluorescence (TXRF) protocol capable of measuring AuNP uptake in cell suspensions, slices of tissues and blood was developed. Specifically, a TXRF method was optimized and compared with the established technique of inductively coupled plasma atomic emission spectroscopy (ICP-AES) using 10 nm reference material AuNPs. TXRF recovery was nearly 100% while ICP-AES underestimated the Au concentration by nearly 80%. This finding has important implications for the accurate assessment of AuNP uptake using the current ICP-AES wet digestion protocols, and it reveals the effectiveness of TXRF protocols developed here. To extend the potential of TXRF for directly quantifying AuNP uptake in tissues, a theoretical model was first developed, and the impact of sample heterogeneity was investigated. This Monte Carlo based simulation toolkit enabled modelling of all the components of benchtop TXRF spectrometer as well the construction of various thin samples. The modeled TXRF spectra yielded nearly 100% recovery of Au regardless of the spatial distribution of the samples. The direct quantification capabilities of TXRF were also demonstrated experimentally using Au solution and AuNPs that were deposited either above or below thin slices of tissue. A 100% quantification accuracy was observed for all sample permutations tested. These findings strongly suggest that direct quantifications of AuNP uptake can be performed on slices of tissue, complementing histological examinations. The capabilities of TXRF for quantifying the concentration of AuNPs in blood was also investigated. Reference Au solutions and AuNPs in whole blood, plasma, and red blood cell suspensions were analyzed with different dilutants and dilution factors. Near 100% recovery was obtained, provided appropriate sample pre-treatment is employed. This offers a fast and easy to use method for quantifying AuNP-blood interactions. This dissertation provides the foundations for TXRF to become a rapid and accurate tool that can contribute to AuNP quantification in tumor cells, tissues and blood, a topic of interest to nanoparticle research.</p>

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Section: Txrf Sample Preparationmentioning

confidence: 99%

Gold Nanoparticle Quantification With Total Reflection X-ray Fluoresence

Mankovskii

2024

Preprint

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“…A preparação direta de soluções diluída de óleo mostrou bons resultados para as concentrações de elementos em níveis de traços superiores a 1 g.g -1 . Liendo et al (1999), descreveram um estudo comparativo entre PIXE e a TXRF para as análises de Cl, K, Ca, Fe, Cu, Zn e Br em fluído amniótico humano. Constataram uma concordância nas medidas realizadas com as duas técnicas para os seguintes elementos: K (100%), Cl (60%), Fe (80%), Cu (50%) e Zn (50%).…”

Section: Fluorescência De Radiação Síncrotron Por Reflexão Total (Txrf)unclassified

Evaluation of reuse water at the Pinheiros Subway Station in the city of São Paulo (Brazil) regarding the presence of metals using X-ray fluorescence technique with syncrotron radiation

Da Silva Melo Júnior,

Aristides Ribeiro,

Da Silva Melo

2023

ISJM

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A garantia da conservação das reservas hídricas é um desafio para toda a humanidade, o que faz com que seja fundamental o estudo de forma continua de novas formas de tratamento e de uso das fontes d’água no planeta. Um dos focos da conservação e sustentabilidade hídrica está no reuso de fontes de água de modo a garantir a continuidade das reservas principais de água potável para atividades de sedentação e produção de alimento. A presente pesquisa teve como intuito pesquisar a qualidade da água utilizada para reuso na estação de metrô Pinheiros na cidade de São Paulo (Brasil) de modo a avaliar o grau quanto à presença de metais pesados e demais elementos químicos pela técnica de fluorescência de raios X por radiação sincrotron do Laboratório Nacional de Luz Síncrotron (LNLS). O levantamento de dados para uma amostra coletada no verão e analisada revelaram um pH de 6,8 e a presença de 19 elementos químicos pela técnica com concentrações na ordem de ng.mL-1, com destaque para a presença de chumbo (Pb).

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“…Процедура отбора проб слюны и десневых жидкостей проста, относительно недорога, имеет низкую вероятность загрязнения. Так как данные по составу слюны или другой жидкости организма дают ценную информацию в области медицины, фармакологии и контроля окружающей среды, для их исследования применяют различные методы анализа в том числе и РФА [104][105][106][107][108][109][110][111][112][113].…”

Section: рентгенофлуоресцентный анализ слюныunclassified

X-Ray Fluorescence Analysis in Biology and Medicine

Ревенко¹

2020

АиК

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X-ray fluorescence analysis (XRF) is widely used when determining the concentrations of elements in various materials in biological research. Current review considers the achievements related to the features of the XRF method. X-ray fluorescence analysis in most cases is a non-destructive method that has proven its potential for measuring the concentrations of elements with a high atomic number Z in organs and tissues in vivo. The main areas of its application in biology and medicine are content determination of basic and toxic elements in plants, samples of bones, teeth, hairs, nails and tissues of vital organs, body fluids such as blood, serum, plasma, saliva, urine, etc. Usually, these studies are part of applied programs that include environmental and metabolic analyses of the population, including the influence of professional factors. The advantages of XRF are also discussed with regards to biological materials. It is noted that the form of the element in the sample practically does not affect the analysis result. In recent years, several new XRF spectrometers models have been designed, where polycapillary lenses and half lenses are used as collimating systems. This is important in the case of in vivo application of X-ray fluorescence determination of certain elements in bones and tissues. The dynamic development is typical for detectors with thermoelectric cooling. Variants of preparation of biological materials for analysis are discussed (grinding, dry or wet ashing, acid decomposition, use of suspensions in the case of XRF with TIR). The results of evaluating the interelement effects for XRF of materials of plant origin, including herbs, spices, apple leaves, birch and tomato leaves, tea, wheat, rye, rice, oatmeal, flax flour, beans, ground, and instant coffee are presented. Examples of the use of XRF in various types of forensic research are considered: poisoning, counterfeiting of drugs and food brands, dental implants, identification of remains. The review presents examples of the participation of Russian X-ray physicists in solving the problems under consideration. The list of references is 400 papers, mainly including the publications of the last 20 years.

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Comparison between proton-induced x-ray emission (PIXE) and total reflection x-ray fluorescence (TXRF) spectrometry for the elemental analysis of human amniotic fluid

Cited by 20 publications

References 11 publications

Gold Nanoparticle Quantification With Total Reflection X-ray Fluoresence

Gold Nanoparticle Quantification With Total Reflection X-ray Fluoresence

Evaluation of reuse water at the Pinheiros Subway Station in the city of São Paulo (Brazil) regarding the presence of metals using X-ray fluorescence technique with syncrotron radiation

X-Ray Fluorescence Analysis in Biology and Medicine

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