Comparison between triple quadrupole, time of flight and hybrid quadrupole time of flight analysers coupled to liquid chromatography for the detection of anabolic steroids in doping control analysis

Comparison of Information-Dependent Acquisition on a Tandem Quadrupole TOF vs a Triple Quadrupole Linear Ion Trap Mass Spectrometer for Broad-Spectrum Drug Screening

“…We also found that QqTOF was slightly less sensitive than QqLIT. Other references have reported a similar trend regarding the sensitivity of high-resolution, untargeted methods compared to nominal mass, targeted methods (9,11,30 ). Although this result may seem surprising, a QqLIT instrument operating in SRM mode is one of the most sensitive methods for targeted analysis because the data acquisition time is dedicated to a specific list of compounds.…”

Comparison of Unit Resolution Srm And Tof-Ms At 12,000 Mass Resolution For Quantitative Bioanalysis of 11 Steroids From Human Plasma

“…However, the use of TOF-MS instruments in quantitative ana lysis started earlier, as the first papers related to the issue are from the beginning of the millennium [16], and the first review about the topic was presented by Williamson in 2007 [17], even though the limitations of linear range with the instruments from the beginning of the millennium were quite severe. Recently, Pozo et al compared 'previous generation' Q-TOF instruments operating at 10,000 mass res olution with QqQ instruments for the ana lysis of anabolical steroids in doping control purposes, and concluded that the limitation of TOF-MS-based approach was its lower sensitivity in comparison with the QqQ instruments [18]. Also recently, Kaufmann et al compared the Orbitrap-based HRMS approach to a QqQ instruments in ana lysis of a high number of veterinary drugs from various matrices, and observed that better sensitivity and higher selectivity were obtained than with neutral loss or precursor ion scanning of QqQ instrument, and that HRMS at 50,000 mass resolution provided equal or even better quantitative performance in comparison to unit mass MS/MS with QqQ instruments [19,20].…”

Mass spectrometric characterization of urinary toremifene metabolites for doping control analyses

“…Additionally, the use of accurate mass measurements has been reported to be useful in several areas of the anti-doping control field like the qualitative detection of doping agents, preventive doping analysis or metabolic studies [9,10]. The combination of LC-MS/MS and accurate mass measurements is a powerful tool for metabolic studies of those compounds which the proton affinity is high enough for electrospray ionization (ESI).…”