Comparison between Tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa

Abstract

<p>The aim of the present study was to compare the effects of tris-buffer and INRA-82 extenders on the quality of cooled rabbit spermatozoa. Pooled semen samples were collected from eight New Zealand White rabbit bucks, divided into three groups and diluted 1:5 with three different semen extenders: INRA-82, tris-citrate glucose (TCG) and tris-citrate trehalose (TCT). Following dilution, the samples were stored at 5°C for up to 48 h. Sperm motility was recorded at 24, 36 and 48 h post-cooling. Furthermore, sperm viability, morphology, membrane integrity, acrosome status and DNA integrity were assessed at 24 h post-cooling. We found that progressive motility percentages were significantly higher (<em>P</em><0.05) in samples diluted in INRA-82 (61.00, 53.50, 44.00% at 24, 36 and 48 h post-cooling, respectively) than those diluted in either TCG (39.25, 32.00, 19.75%) or in TCT (47.25, 40.50, 29.00%). We also reported that sperm viability, percentage of normal spermatozoa, percentage of spermatozoa with intact acrosome and DNA integrity after 24 h cooling were significantly higher (<em>P</em><0.05) in INRA-82 diluted samples compared to TCG and TCT diluted ones. In summary, our results show that dilution of rabbit semen in INRA-82 improved sperm physiological parameters post-cooling compared to those diluted in TCG or TCT. Our findings also suggest that INRA-82 is a promising diluent that can be used effectively to maintain the viability of chilled rabbit semen.</p>

A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

“…In this study, Kacang goat semen viability was extended using an SM-EY extender [ 14 ]. Skim milk contains sulfhydryl, β -lactoglobulin, phosphocaseinate, and casein micelle, which protect the plasma membrane from lipid peroxidation [ 37 ]. Egg yolk is rich in cholesterol [ 38 ], which improves the quality of stored semen by decreasing mitochondrial deterioration and inhibiting ROS production [ 39 ].…”

Green tea extract increases the quality and reduced DNA mutation of post-thawed Kacang buck sperm

“…The sulfhydryl groups, β-lactoglobulin, and phosphocaseinase of skim milk prevent sperm from the harmful effects of sperm metabolism byproducts themselves. The casein micelle of skim milk maintains plasma membrane stability and protects sperm from the ROS action [ 13 ]. Cholesterol derived from egg yolk maintains plasma membrane stability [ 14 ] and inhibits DNA damage [ 15 ].…”

Improvement in quality and storage ability of rabbit semen by using black or thyme seed as dietary supplementation

“…Artificial insemination improves genetic selection and fertility, even during times of stress (Bergonzoni et al, 1994). In most rabbit farms, fresh diluted semen (Campaniello et al, 2010;Fadl et al, 2020) or chilled semen are used, due to it is low cost and high survival rate compared to cryopreservation (Di Iorio et al, 2014). Spermatozoa are rich in polyunsaturated fatty acids with low antioxidant levels within the cytoplasm (Ismail et al, 2020;Zeweil & El-Gindy, 2017).…”