Comparison between whole mount tissue preparations and virtual tissue microarray samples for measuring Ki-67 and apoptosis indices in human bladder cancer

Oshiro, Hisashi; Czerniak, Bogdan; Sakamaki, Kentaro; Tsuta, Koji; Bondaruk, Jolanta; Keyhani, Afsaneh; Dinney, Colin P.; Nagai, Takeshi; Kamat, Ashish M.

doi:10.1097/md.0000000000004500

Cited by 2 publications

(2 citation statements)

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“…Other plausible explanations for these differences are the pathological sample processing from surgical removal to immunohistochemistry staining, differences in cut-off levels, and sample size (168,(219)(220)(221)(222). The use of TMA in immunohistochemical studies of invasive urinary bladder cancer might be questionable as intra-tumor heterogeneity might affect the results compared with whole section, which might be due to sampling only small areas of the tumor (173,223).…”

Section: Discussionmentioning

confidence: 99%

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Staging and tumor biological mechanisms of lymph node metastasis in invasive urinary bladder cancer

Aljabery¹

2017

Linköping University Medical Dissertations

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Aim: To study the possibility of detecting lymph node metastasis in locally advanced urinary bladder cancer (UBC) treated with radical cystectomy (RC) by using preoperative positron emission tomography/computed tomography (PET/CT) and peroperative sentinel node biopsy (SNB) technique. We also investigate the clinical significance of macrophage traits expression by cancer cells, M2-macrophage infiltration (MI) in tumor stroma and the immunohistochemical expression of biomarkers in cancer cells in relation to clinico-pathologic data. Patients and Methods:We studied prospectively 122 patients with UBC, pathological stage pT1-pT4 treated with RC and pelvic lymph node dissection (PLND) during [2005][2006][2007][2008][2009][2010][2011] at the Department of Urology, Linköping University Hospital. In the first study, we compared the results of preoperative PET/CT and conventional CT with the findings of postoperative histopathological evaluation of lymph nodes (LNs). In the second study we investigated the value of SNB technique for detecting pathological LNs during RC in patients with UBC. We also examined the significance of the primary tumor location in the bladder in predicting the site of LN metastases, and the prognostic significance of lympho-vascular invasion (LVI) and lymph node metastasis density (LNMD) on survival. In the third study, we investigate the clinical significance of macrophage infiltration (MI) in tumor stroma and macrophage-traits expression by tumor cells. In the fourth study, we investigate the cell cycle suppression pro-

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Section: Discussionmentioning

confidence: 99%

“…For each patient, the core with the highest value was considered to be representative for that individual. This strategy was applied, because there is always a risk that a hot spot in a tumor will be missed in a TMA core, as studied by others (171)(172)(173).…”

Section: Discussionmentioning

confidence: 99%