Comparison capacity of collagen hydrogel, mix‐powder and in situ hydroxyapatite/collagen hydrogelscaffolds with and without mesenchymal stem cells and platelet‐rich plasma in regeneration of critical sized bone defect in a rabbit animal model

Abstract

The aim of this study was to investigate the effect of developed collagen (Co) hydrogel (CH), powder-mixed hydroxyapatite/collagen (HA/Co) hydrogel and in situ synthesized HA/Co (In/HA/Co) hydrogel with or without mesenchymal stem cell (MSC) and platelet-rich plasma (PRP) on the regeneration of fullthickness critical size bone defect in the rabbit animal model. In the first step of this study, the scaffolds were synthesized and characterized using FTIR spectroscopy, X-ray diffraction, and scanning electron microcopy. In the second step or animal study, the radial bone defects were filled with the synthesized scaffolds with and without MSC and PRP. One hundred sixty one year-old New Zealand white male rabbits were randomly divided in 16 groups of 10 rabbits including control

Remotely Temporal Scheduled Macrophage Phenotypic Transition Enables Optimized Immunomodulatory Bone Regeneration

“…It is worth mentioning that the C and CG groups also showed limited osteogenic effects in both 2D and 3D culture models (Figures 4B–F and 5B–E) without obvious macrophage polarization toward M2, which was consistent with previous studies. [ 27a,30 ] As reported, type I collagen, acting as a regulator of osteogenic differentiation and cell adhesion, [ 27b,31 ] has the ability to guide a development toward the bone cell phenotype, [ 27c ] which may be partially achieved by modulating the [Ca 2+ ] i‐ and cAMP‐signaling pathways through altering PCK activity. [ 32 ] Collectively, these data suggest that macrophage polarization by magnetized hydrogels under an MF effectively promoted BMSC osteogenic differentiation, which shows potential in the development of novel immunomodulatory approaches for bone regeneration.…”

Evaluation of Bone Repair Using a New Biphasic Synthetic Bioceramic (Plenum® Osshp) in Critical Calvaria Defect in Rats

“…Hydroxyapatite has similarities to bone tissue and is widely applied as a grafting material in bone regeneration surgeries due to its excellent biocompatibility and osteoconductive characteristics [8,[16][17][18]. Beta tricalcium phosphate is one of the most attractive bone repair materials for regenerative areas, has excellent osteoconductive and osteotransductive properties, and has a crystalline structure of simple degradation and thermal stability [1,19,20].…”

Recent Achievements in the Development of Biomaterials Improved with Platelet Concentrates for Soft and Hard Tissue Engineering Applications

“…Although there are some controversial results of platelet-rich preparations on the regenerative processes of bone tissue [193,194], in general, recent reports show a beneficial effect of PRP in combination with biomaterials on the regeneration of bone tissue (Figure 9). It was found that biomaterials enriched with platelet-rich preparations promoted the adhesion, viability, proliferation and osteogenic differentiation of osteoblasts and stem cells.…”

“…Thus, these promising in vitro results showed that the addition of PRP to the CDHA/collagen scaffold promoted its cytocompatibility and osteogenic potential; however, additional in vivo research should be performed in order to confirm this phenomenon. Bakhtiarimoghadam et al [194] developed collagen/powder mixed HAp (HAp/Col) and in situ synthesized a collagen/hydroxyapatite hydrogel (In/HAp/Col), and they evaluated their biocompatibility in vivo with or without the addition of rabbit BMSCs and PRP. The study was performed by using New Zealand rabbits with full-thickness critical-size radial bone defects.…”