Comparison in sensitivity of 10 HIV antibody detection tests by serial dilutions of Western blot-confirmed samples

Stute, R.

doi:10.1016/0166-0934(88)90130-9

Cited by 9 publications

(3 citation statements)

References 4 publications

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“…The sensitivities of the ELISAs currently used for screening of HIV-1-specific antibodies have been found to be low, especially during the period of seroconversion after infection (7,31) and also with dilution series of confirmed positive specimens (39). When coded quality-control panels were tested by the recombinant TR-FIA and ELISA, a higher sensitivity was obtained by the recombinant TR-FIA (Table 3).…”

Section: Discussionmentioning

confidence: 99%

New sensitive and specific assay for human immunodeficiency virus antibodies using labeled recombinant fusion protein and time-resolved fluoroimmunoassay

Siitari¹,

Turunen²,

Schrimsher³

et al. 1990

J Clin Microbiol

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A new, rapid method for the detection of human immunodeficiency virus type 1 (HIV-1) antibody by time-resolved fluoroimmunoassay (TR-FIA) was developed. In this assay format, microtitration strips were coated with a recombinant fusion protein, and the same protein was labeled with europium and added into the wells simultaneously with the test specimens. The recombinant fusion protein contained the HIV-1 p24 gag protein sequence that carried an insertion, near the carboxyl terminus, of a 23-amino-acid sequence from a highly conserved region of the HIV-1 gp41 envelope protein. This recombinant antigen enabled the detection of antibodies to both gag and env gene products. When this assay was compared with a commercially available recombinant enzyme-linked immunoabsorbent assay (ELISA) by using four quality-control panels, the TR-FIA detected all 20 positive specimens, while the recombinant ELISA detected only 16 of them. This increased sensitivity could be demonstrated directly by the assay of dilution series of HIV-1-positive sera. The analysis of two seroconversion panels by TR-FIA and six ELISAs showed that TR-FIA allowed detection of antibody in infected individuals 16 days earlier than the other assays did. In addition to being highly sensitive, the assay was highly specific; of the 57 samples shown to be repeatedly positive by ELISA but known to be HIV-1 negative by Western immunoblot analysis, only 1 sample reacted positively in this assay. The specificity of the assay was 99.9% when 1.054 random serum specimens were tested.

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Section: Discussionmentioning

confidence: 99%

New sensitive and specific assay for human immunodeficiency virus antibodies using labeled recombinant fusion protein and time-resolved fluoroimmunoassay

Siitari¹,

Turunen²,

Schrimsher³

et al. 1990

J Clin Microbiol

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“…Zwischen den acht anti-HIV-1-Tests, die von der Food and Drug Administration (FDA) zugelassen waren, wurden ebenfalls unterschiedliche Ergebnisse festgestellt (4). In anderen Studien, die verschiedene kommerzielle Tests zum Nachweis von anti-HIV-1 verglichen, konnten · auch Unterschiede in der diagnostischen Sensitivität und Spezifität festgestellt werden (1,5), wobei für die Bestimmung der Sensitivität häufig Tltrationsexperimente durchgeführt wurden (6,7,8). Ein Ringversuch der Blutspendedienste und Laboratorien, bei dem die Sensitivität und Spezifität mit 13 kommerziellen ELISAs zum Nachweis von anti-HIV-1-Antikörpern bestimmt wurde, zeigte, daß die Tests sehr verläßlich sind mit einer durchschnittlichen diagnostischen Sensitivität und Spezifität von 99,5% (9).…”

Section: Materials Und Methodenunclassified

Vergleich von fünf kombinierten anti-HIV-1/2-ELISA mit zwei verschiedenen Serokonversionspanels

Koerner¹,

Kilian²

1990

LaboratoriumsMedizin

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“…Researchers have used serial dilutions of Western blot confirmed HIV antibody positive sera to compare the sensitivity of various tests and have found some assays to be 10-1000 times more sensitive than others. [1] FDA recommends use of dilutional analysis of known positives for comparing the analytical sensitivity of different HIV antibody assays. [2] Our present work is an expansion of these studies and was designated to compare the performance of two different HIV diagnostic ELISA kits using dilutional panel.…”

Section: Introductionmentioning

confidence: 99%

Comparison of analytical sensitivity of HIV diagnostic kits using dilutional panel

Bindra¹,

Chhabra²,

Sharma³

et al. 2016

Int J of Biomed & Adv Res

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An array of tests have been developed over a period of time for screening and diagnosis of HIV infection which include tests for detection of antigen and antibody, viral nucleic acid and the virus itself. Amongst the available tests, serodiagnosis by ELISA is widely being used. It offers a relatively inexpensive procedure with ease of performance and ability to effectively screen a large number of specimens. Our current study is designed to compare the performance of two different HIV diagnostic kits having different coated antigens using HIV dilutional panel. Kit 'X' had synthetic peptides (3rd generation), and the Kit 'Y' had recombinant antigens (2nd generation) coated on the microtitre plates. 10 HIV-1 plasma panel members which were collected from various blood banks were used for comparison of their performance. The plasma samples were diluted in human plasma (base matrix) negative for anti HIV, anti HCV, HBsAg, VDRL and HTLV I/II. Serial dilutions in the range of 10 to10,000 fold for each sample were used for this study. These kits were compared in terms of antibody titre which is defined as the dilution at which the sample OD to cut off (S/Co) value is 1. It was seen that Kit 'X' is more sensitive as compared to Kit 'Y' for all the HIV panel members. This indicates that Kit 'X' had better analytical sensitivity performance as compared to Kit 'Y' since the S/Co ~ 1.0 of Kit 'X' is at a higher dilution (1:1000) than of Kit 'Y' (1:100). Thus this indicates that dilutional panel can help the manufacturers to design a diagnostic kit which is able to detect the infection at an early stage of disease. It was seen that the S/Co ~ 1.0 of kit X at a higher dilution

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Comparison in sensitivity of 10 HIV antibody detection tests by serial dilutions of Western blot-confirmed samples

Cited by 9 publications

References 4 publications

New sensitive and specific assay for human immunodeficiency virus antibodies using labeled recombinant fusion protein and time-resolved fluoroimmunoassay

New sensitive and specific assay for human immunodeficiency virus antibodies using labeled recombinant fusion protein and time-resolved fluoroimmunoassay

Vergleich von fünf kombinierten anti-HIV-1/2-ELISA mit zwei verschiedenen Serokonversionspanels

Comparison of analytical sensitivity of HIV diagnostic kits using dilutional panel

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