Comparison of 1.7 mm submicro and 3 mm micro gradient NMR probes for the acquisition of1H13C and1H15N heteronuclear shift correlation data

Martin, Gary E.; Hadden, Chad E.

doi:10.1002/(sici)1097-458x(199910)37:10<721::aid-mrc525>3.0.co;2-z

Cited by 25 publications

(5 citation statements)

References 15 publications

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“…A comparison between 5 and 3 mm NMR probes yielded an approximate factor of 2 improvement in signal to noise for the 3 mm probe [75]. A similar comparison between 3 and 1.7 mm probes resulted in another 2.4 factor improvement in signal-to-noise for the 1.7 mm probe [76]. Similar improvements in signal-to-noise can be achieved by using 3 or 1.7 mm NMR tubes in a standard 5 mm NMR probe.…”

Section: Sample Size and Limit Of Detectionmentioning

confidence: 61%

Quantitative NMR-Based Biomedical Metabolomics: Current Status and Applications

2020

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Nuclear Magnetic Resonance (NMR) spectroscopy is a quantitative analytical tool commonly utilized for metabolomics analysis. Quantitative NMR (qNMR) is a field of NMR spectroscopy dedicated to the measurement of analytes through signal intensity and its linear relationship with analyte concentration. Metabolomics-based NMR exploits this quantitative relationship to identify and measure biomarkers within complex biological samples such as serum, plasma, and urine. In this review of quantitative NMR-based metabolomics, the advancements and limitations of current techniques for metabolite quantification will be evaluated as well as the applications of qNMR in biomedical metabolomics. While qNMR is limited by sensitivity and dynamic range, the simple method development, minimal sample derivatization, and the simultaneous qualitative and quantitative information provide a unique landscape for biomedical metabolomics, which is not available to other techniques. Furthermore, the non-destructive nature of NMR-based metabolomics allows for multidimensional analysis of biomarkers that facilitates unambiguous assignment and quantification of metabolites in complex biofluids.

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Section: Sample Size and Limit Of Detectionmentioning

confidence: 61%

Quantitative NMR-Based Biomedical Metabolomics: Current Status and Applications

2020

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“…The other factor for enhancing sensitivity is to reduce the sample volume by concentrating the solution. A variety of micro-and submicroprobes of less than 5 mm in diameter 90) are commercially available. Combined use of such probes with Shigemi NMR micro cells 91,92) achieves a further reduction in the sample volume.…”

Section: Application Of Long-range Coupling Constants For Determining...mentioning

confidence: 99%

Advanced NMR Approaches for a Detailed Structure Analysis of Natural Products

Fukushi¹

2006

Bioscience, Biotechnology, and Biochemistry

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Some new nuclear magnetic resonance (NMR) approaches to elucidate chemical structures, which have not been determined by routine NMR methods, are presented. Selective detection of methine (CH), methylene (CH(2)), or methyl (CH(3)) signals in each subspectrum by editing NMR methods was utilized to reduce the complexity in crowded spectra. It also increased the peak separation and enhanced the sensitivity by limiting the measuring area of the 2D spectra. Several 2D methods to measure (2,3)J(CH) values, which are useful for stereochemical assignment are then introduced. To determine the structure of a highly hydrogen-deficient molecule, efficient correlation methods for long-range (13)C-(13)C coupling and (1)H-(15)N HMBC are also described.

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“…82 This group also compared micro (3 mm) and submicro (1.7 mm) probes and saw a 2.3-fold improvement in the signal-to-noise ratio for the submicro probe in a GHSQC experiment. 83 Complexes with transition metals…”

Section: Miscellaneous Topicsmentioning

confidence: 99%