Comparison of 2 different antibody assay methods, Elecsys Anti-HCVII (Roche) and Vidas Anti-HCV (Biomerieux), for the detection of antibody to hepatitis C virus in Egypt

Gaballah, A.M.; Esawy, Marwa M.

doi:10.1016/j.diagmicrobio.2018.05.013

Cited by 7 publications

(5 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can be argued that the level of false positivity is low in this study since Pakistan is a region of high endemicity for HCV. The false positivity rate was found to be 24.4% in a study conducted in Egypt (15). The reason for these low levels may be that the region is hyperendemic for HCV (prevalence 21 -24%) and that patient population consists mostly of blood donors.…”

Section: Discussionmentioning

confidence: 99%

Updating the Signal-to-cutoff Level to Reduce Anti-hepatitis C Virus False Positivity

Öztürk

Ağalar

2021

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Anti-hepatitis C virus (anti-HCV) is the only screening test being used in the diagnosis of hepatitis C. In this study, we examined anti-HCV positivity rates in our hospital. Objectives: The aim of administering the anti-HCV test was to distinguish patients with hepatitis C infection from false positivity in patients with reactive results. Methods: The anti-HCV tests were performed at Fatih Sultan Mehmet Training and Research Hospital in Istanbul, Turkey, between January 1, 2015 and December 31, 2019. The patients were evaluated retrospectively in terms of age, gender, anti-HCV titer, the clinic for which the examination was requested, the reason for the examination, and the history of hepatitis C. Results: In this study, 511 patients who had two negative polymerase chain reaction (PCR) results were evaluated as false positive cases and enrolled. The cut-off value was found to be 7.5 IU/ml, with the highest sensitivity of 94.4% and specificity of 94.5% (area under the curve [AUC]: 0.982). The lowest anti-HCV titer (5.2) was from patients without acute hepatitis, who were HCV-RNA positive and diagnosed with chronic hepatitis C. Conclusions: It may be more appropriate to report anti-HCV cut-off value of 0 - 5 as negative, 5 - 7.5 as borderline, and > 7.5 as positive. Working with a more acceptable cut-off level with a greater number of tests can help identify patients with asymptomatic HCV infection. Also, it can possibly reduce the cost due to a decrease in the number of PCR tests administered.

show abstract

Section: Discussionmentioning

confidence: 99%

Updating the Signal-to-cutoff Level to Reduce Anti-hepatitis C Virus False Positivity

Öztürk

Ağalar

2021

Jundishapur J Microbiol

View full text Add to dashboard Cite

show abstract

“…A similar finding is observed in other studies. [15][16][17] A positive HCV Ab result may be seen in patients with current or resolved HCV infection. However, false HCV Ab positive results may be found if the reliability of assay is compromised.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Hepatitis C Antibody Assays and Evaluation of Agreement Between Hepatitis C Antigen and PCR Results

Iqbal

Baig

Naseem

et al. 2022

J Ayub Med Coll Abbottabad

View full text Add to dashboard Cite

Background: Hepatitis C is associated with a wide range of health repercussions. Pakistan is one of the highly prevalent countries of Hepatitis C Virus (HCV) infection. The availability of cost-effective, robust, and reliable screening and diagnostic tests for hepatitis C is important to address the disease burden. Standardization of screening and diagnostic assays in clinical laboratories is crucial for achieving big goals. Objectives of this study are to correlate the results of two different HCV antibody (HCV Ab) assays and to examine the correlation of HCV core antigen (HCV c Ag) results with HCV PCR for HCV infection diagnosis. Methods: This descriptive cross-sectional study was carried out from November to December 2020 at Dow University of Health Sciences. Total number of 40 HCV Ab samples were analysed by both chemiluminescence (CMIA) and electrochemiluminescence (ECLIA) immunoassays. Tests for HCV RNA PCR and HCV c Ag were performed on all samples. Results of screening and diagnostic assays were correlated and agreements were examined. Statistical analysis for agreement was carried out by using R software version 3.6.3 through AC1 Gwetz Statistic. The study was approved by the institutional ethical review committee. Results: An agreement of 0.73 and 0.95 was found between two different HCV Ab immunoassays and HCV c Ag and HCV PCR, respectively. Conclusion: We found a good correlation between CMIA and ECLIA for HCV Ab. An excellent correlation was found between HCV c Ag and HCV PCR. Based on our study findings, HCV c Ag is a candidate test for the diagnosis of active HCV infection.

show abstract

“…To date, early screening and diagnosis of HCV infection are based on three different methods, which may be utilized alone or in combination. These are (a) detection of HCV core antigen (HCV‐Ag); (b) nucleic acid testing (NAT) to detect HCV‐RNA by PCR; and (c) detection of an IgG antibody by ELISA (anti‐HCV) 23 . Related literature reports that there is a good correlation between the contents of HCV‐Ag and the quantity of HCV‐RNA as viral load exceeds 3000 IU/mL in the serum samples of HCV patients, so HCV core antigen can be used as early diagnostic indicators of HCV infection 24 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of double antigen sandwich and indirect enzyme‐linked immunosorbent assay for the diagnosis of hepatitis C virus antibodies

Qin

Sha

Feng

et al. 2020

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background: The aim of this study is to compare double-antigen sandwich enzymelinked immunosorbent assay (ELISA) and indirect ELISA in the diagnosis of hepatitis C virus(HCV)infection. Methods and materials: A total of 176 samples from the Tumor Hospital Affiliated to Xin Jiang Medical University were utilized to comparison. All serum samples were tested using double-antigen sandwich ELISA and indirect ELISA. Cohen's kappa statistics were used to assess the agreement between the two assays, and multivariate analysis was used to evaluate risk factors for the discordance between the doubleantigen ELISA and indirect ELISA. Results: The positivities of indirect ELISA (Beijing Wantai), double-antigen sandwich ELISA (Beijing Wantai), and indirect ELISA (Beijing Jinhao) were 74.43%, 68.75%, and 73.30%, respectively. The agreement between the indirect ELISA (Beijing Wantai) and double-antigen sandwich ELISA (Beijing Wantai) was high (κ = 0.829;P < .001), and the agreement between the double-antigen sandwich ELISA (Beijing Wantai) and indirect ELISA (Beijing Jinhao) was high (κ = 0.847;P < .001). Variables associated with discordant results between the double-antigen sandwich and indirect ELISA in multivariate analysis were as follows: female (OR:1.462; P < .05), age (<35 years old; OR:3.667; P < .05), and cancer (suffer from malignant tumor; OR:3.621; P < .05). Conclusion: In detection of HCV, high agreement was found between the doubleantigen sandwich ELISA and indirect ELISA. Female, younger age, and suffer from malignant tumor were significant risk factors for the discordance. Based on doubleantigen sandwich ELISA has distinct methodological advantages over indirect ELISA. It is recommended for the diagnosis of HCV infection.

show abstract

Comparison of 2 different antibody assay methods, Elecsys Anti-HCVII (Roche) and Vidas Anti-HCV (Biomerieux), for the detection of antibody to hepatitis C virus in Egypt

Cited by 7 publications

References 21 publications

Updating the Signal-to-cutoff Level to Reduce Anti-hepatitis C Virus False Positivity

Updating the Signal-to-cutoff Level to Reduce Anti-hepatitis C Virus False Positivity

Comparison of Hepatitis C Antibody Assays and Evaluation of Agreement Between Hepatitis C Antigen and PCR Results

Comparison of double antigen sandwich and indirect enzyme‐linked immunosorbent assay for the diagnosis of hepatitis C virus antibodies

Contact Info

Product

Resources

About