Comparison of a commercial ELISA kit and a neutralization assay for assessment of humoral immunity to measles virus

Friedman, Maureen G.; Romanova, S. Yu.; Galil, Aharon; Sarov, Batia; Dagan, Ron

doi:10.1016/s0888-0786(96)01064-5

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…Testing with a variety of assay methods (such as haemagglutination inhibition, neutralizing test, enzyme immunoassay) can provide very different results [6]. Even investigating the same sera with a variety of kits based on the same method, can lead to significantly different results [3].…”

Section: Discussionmentioning

confidence: 99%

The European Sero-Epidemiology Network: standardizing the enzyme immunoassay results for measles, mumps and rubella

Andrews¹,

Pebody²,

Berbers

et al. 2000

Epidemiol. Infect.

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The ESEN (European Sero-Epidemiology Network) project was established to harmonize the seroepidemiology of five vaccine preventable infections including measles, mumps and rubella in eight European countries. This involved achieving comparability both in the assay results from testing in different centres and also sampling methodology. Standardization of enzyme immunoassay results was achieved through the development of common panels of sera by designated reference centres. The panels were tested at the reference laboratory and then distributed to each participating laboratory for testing using their routine methods. Standardization equations were calculated by regressing the quantitative results against those of the reference laboratory. Our study found large differences in unitage between participants, despite all using an EIA method standardized against an international or local standard. Moreover, our methodology adjusted for this difference. These standardization equations will be used to convert the results of main serosurvey testing into the reference country unitage to ensure inter-country comparability.

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Section: Discussionmentioning

confidence: 99%

The European Sero-Epidemiology Network: standardizing the enzyme immunoassay results for measles, mumps and rubella

Andrews¹,

Pebody²,

Berbers

et al. 2000

Epidemiol. Infect.

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Sero-epidemiology of measles-specific IgG antibodies and predictive factors for low or missing titres in a German population-based cross-sectional study in children and adolescents (KiGGS)

Poethko‐Müller

Mankertz

2011

Vaccine

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Background and objective:In the European Region, measles elimination is now targeted to 2015. To measure progress towards elimination age-group specific susceptibility targets have been defined. Age-specific measles susceptibility in children and adolescents was evaluated in Germany. Taking into account a broad range of socio-demographic, health-and vaccination status related variables, populations for vaccination campaigns were identified. MethodWe analysed data from children aged 1 to 17 years in the representative German Health Interview and Examination Survey for Children and Adolescents (KiGGS). Measles immunoglobulin G antibodies were measured in 13,977 participants by enzyme immunoassay (ELISA). Bivariate and multivariate logistic regression analyses were used to determine parental and infant related factors associated with measles susceptibility. ResultsThe overall prevalence of seronegativity in children tested for measles IgG aged 1 to 17 years was 10.0% (95% CI 9.4-10.7). The prevalence of seronegativity in the German population was below the WHO targets for measles elimination in children aged 2 to 9 year-olds but exceeded the target for 10 to 17 year-olds. Age differences in the level of seronegativity were found to be mainly due to differences in vaccination coverage. A higher level of susceptibility was observed if parents did not comply with the request to present the child's vaccination card. In vaccinated children, immigration, male gender, very young age at first vaccination and a longer time period since last vaccination were associated with a higher level of susceptibility. ConclusionFurther increase of the two-dose vaccination coverage is necessary in order to achieve the WHO targets. Catch up vaccination campaigns should focus on adolescents and immigrants. 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

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Immune response after primary and re-vaccination with different combined vaccines against measles, mumps, rubella

Tischer

Gerike

2000

Vaccine

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Comparison of a commercial ELISA kit and a neutralization assay for assessment of humoral immunity to measles virus

Cited by 3 publications

References 8 publications

The European Sero-Epidemiology Network: standardizing the enzyme immunoassay results for measles, mumps and rubella

The European Sero-Epidemiology Network: standardizing the enzyme immunoassay results for measles, mumps and rubella

Sero-epidemiology of measles-specific IgG antibodies and predictive factors for low or missing titres in a German population-based cross-sectional study in children and adolescents (KiGGS)

Immune response after primary and re-vaccination with different combined vaccines against measles, mumps, rubella

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