Comparison of a commercial ELISA and indirect hemagglutination assay with the modified agglutination test for detection of Toxoplasma gondii antibodies in giant panda (Ailuropoda melanoleuca)

Yue, Chanjuan; Yang, Wanjing; Li, Yun-Li; Zhang, Dongsheng; Lan, Jingchao; Su, Xiaoyan; Li, Lin; Liu, Yiyan; Zheng, Weichao; Wu, Kongju; Fan, Xueyang; Yan, Xia; Hou, Rong; Liu, Songrui

doi:10.1016/j.ijppaw.2022.07.001

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…To the best of our knowledge, this is the first large-scale study to survey the seroprevalence and analyze the risk factors for the presence of T. gondii in the captive panda population. As the detection of the T. gondii infection in host primarily relies on serological assays, the serological methods used in this study were selected from our previous studies by comparing five commercial kits ( Yue et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalence and risk factors of Toxoplasma gondii infection in captive giant panda (Ailuropoda melanoleuca)

Yue

Yang

Fan

et al. 2022

Front. Cell. Infect. Microbiol.

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IntroductionToxoplasma gondii, a globally zoonotic protozoan parasite, infects most warm-blooded animals including the giant panda, and poses a serious threat to the giant panda conservation. However, the seroprevalence and the risk factors for toxoplasmosis in giant pandas are unknown. Here we aimed to determine the seroprevalence of T. gondii in the captive population of giant pandas and analyze the factors associated with the increased risk of infection.MethodsA total of 203 serum samples were collected from 157 (95 females and 62 males) captive giant pandas from 2007 to 2022, antibodies against T. gondii were screened using commercial ELISA and MAT kits.ResultsThe results showed 56 (35.67%) giant pandas were seropositive, age and transfer history between institutions were identifified as risk factors for T. gondii infection. It is suggested that age-related seroprevalence was the main factor, and housing multiple species in the same environment may increase the chance of cross-infection of T. gondii.DiscussionThis study can provide research data for developing policies for the prevention and control of T. gondii and protecting the health of captive giant pandas and other wildlife.

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Section: Discussionmentioning

confidence: 99%

“…If the two tests showed different results, the sample needed further verification. All of the tests were performed according to the methods described in our previous study ( Yue et al., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Seroprevalence and risk factors of Toxoplasma gondii infection in captive giant panda (Ailuropoda melanoleuca)

Yue

Yang

Fan

et al. 2022

Front. Cell. Infect. Microbiol.

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Investigation of Toxoplasma gondii Infection in Yunnan Semi-fine Wool Sheep (Ovis aries) and wild Rodents in Yunnan, China

Li,

Cheng,

Deng

et al. 2024

Acta Parasit.

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Establishment and application of an iELISA detection method for measuring apical membrane antigen 1 (AMA1) antibodies of Toxoplasma gondii in cats

Gao,

Shen,

Fan

et al. 2023

BMC Vet Res

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Background Diseases caused by Toxoplasma gondii (T. gondii) have introduced serious threats to public health. There is an urgent need to develop a rapid detection method for T. gondii infection in cats, which are definitive hosts. Recombinant apical membrane antigen 1 (rAMA1) was produced in a prokaryotic expression system and used as the detection antigen. The aim of this study was to evaluate and optimize a reliable indirect enzyme-linked immunosorbent assay (iELISA) method based on rAMA1 for the detection of antibodies against T. gondii in cats. Results The rAMA1-iELISA method was developed and optimized by the chessboard titration method. There were no cross-reactions between T. gondii-positive cat serum and positive serum for other pathogens, indicating that rAMA1-iELISA could only detect T. gondii in most cases. The lowest detection limit of rAMA1-iELISA was 1:3200 (dilution of positive serum), and the CV of repeated tests within batches and between batches were confirmed to be less than 10%. The results of 247 cat serum samples detected by rAMA1-iELISA (kappa value = 0.622, p < 0.001) were in substantial agreement with commercial ELISA. The ROC curve analysis revealed the higher overall check accuracy of rAMA1-iELISA (sensitivity = 91.7%, specificity = 93.6%, AUC = 0.956, 95% CI 0.905 to 1.000) than GRA7-based iELISA (sensitivity = 91.7%, specificity = 85.5%, AUC = 0.936, 95% CI 0.892 to 0.980). Moreover, the positive rate of rAMA1-iELISA (6.5%, 16/247) was higher than that of GRA7-based iELISA (3.6%, 9/247) and that of commercial ELISA kit (4.9%, 12/247). Conclusion The iELISA method with good specificity, sensitivity, and reproducibility was established and can be used for large-scale detection of T. gondii infection in clinical cat samples.

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Comparison of a commercial ELISA and indirect hemagglutination assay with the modified agglutination test for detection of Toxoplasma gondii antibodies in giant panda (Ailuropoda melanoleuca)

Cited by 4 publications

References 14 publications

Seroprevalence and risk factors of Toxoplasma gondii infection in captive giant panda (Ailuropoda melanoleuca)

Seroprevalence and risk factors of Toxoplasma gondii infection in captive giant panda (Ailuropoda melanoleuca)

Investigation of Toxoplasma gondii Infection in Yunnan Semi-fine Wool Sheep (Ovis aries) and wild Rodents in Yunnan, China

Establishment and application of an iELISA detection method for measuring apical membrane antigen 1 (AMA1) antibodies of Toxoplasma gondii in cats

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