Comparison of a Conserved Region in Fowlpox Virus and Vaccinia Virus Genomes and the Translocation of the Fowlpox Virus Thymidine Kinase Gene

Binns, M. M.; Tomley, Fiona M.; Campbell, Joan; Boursnell, M. E. G.

doi:10.1099/0022-1317-69-6-1275

Cited by 40 publications

(28 citation statements)

References 25 publications

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“…1 and 2a). The ORFs are situated in a tightly clustered configuration with a dominant direction of orientation similar to previously reported nucleotide sequences from various VV and FPV genomic regions (Drillien et al, 1987;Binns et al, 1988;Tomley et al, 1988;Niles et al, 1986;Rosel et al, 1986;Schmitt & Stunnenberg, 1988;Tamin et al, 1988;Kotwal & Moss, 1988, 1989Boursnell et al, 1988). Only two regions within the 10465 bp sequence have any significant stretches that lack potential ORFs.…”

Section: Discussionsupporting

confidence: 80%

“…From the nucleotide sequence provided in this communication and others reported previously (Drillien et al, 1987;Binns et al, 1988;Tomley et al, 1988), it is apparent that FPV and VV are closely related in at least some genomic regions. Previous reports have found that FPV contains a genomic region closely related to a portion of the VV HindlII J region (Drillien et al, 1987;Binns et al, 1988).…”

Section: Discussionsupporting

confidence: 56%

“…2a). The arrangement of tightly clustered ORFs oriented in a dominant direction is reminiscent of the organization in many VV genomic regions (Niles et al, 1986: Rosel et al, 1986Schmitt & Stunnenberg, 1988;Tamin et al, 1988;Kotwal & Moss, 1988, 1989Boursnell et al, 1988) and in the limited number of FPV genomic regions analysed (Drillien et al, 1987;Binns et al, 1988;Tomley et al, 1988).…”

Section: Nucleotide Sequence Analysis Of the 105 Kbp Fpv Hindlil Framentioning

confidence: 99%

“…Recently, however, the nucleotide sequence of a 3-1 kbp fragment (Drillien et al, 1987), a 6.1 kbp fragment and an 11.2 kbp BamHI FPV genomic fragment were reported and certain predicted open reading frames (ORFs) were found to be homologous to VV ORFs. Analysis of the 3-1 kbp and 6.1 kbp fragments revealed extensive conservation between the FPV genomic segment and the VV HindlII J region at the amino acid sequence level and in the organization of the ORFs (Drillien et al, 1987;Binns et al, 1988). An FPV homologue of the VV thymidine kinase (TK) gene was, however, lacking from the HindlII J equivalent region.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nucleotide sequence analysis of a 10.5 kbp HindIII fragment of fowlpox virus: relatedness to the central portion of the vaccinia virus HindIII D region

Tartaglia

Winslow

Goebel

et al. 1990

Journal of General Virology

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The nucleotide sequence of a 10465 bp HindlII genomic fragment from fowlpox virux (FPV) is presented. Analysis of the nucleotide sequence revealed 10 potential major open reading frames (ORFs). Five of these ORFs are predicted to encode polypeptides with significant homology to hypothetical polypeptides derived from nucleotide sequence analysis of the vaccinia virus (VV) HindlII D region. Interestingly, these homologous ORFs do not occur in the same tandem arrangement in the FPV genome as they do in the VV genome. These results are discussed.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 56%

Section: Nucleotide Sequence Analysis Of the 105 Kbp Fpv Hindlil Framentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nucleotide sequence analysis of a 10.5 kbp HindIII fragment of fowlpox virus: relatedness to the central portion of the vaccinia virus HindIII D region

Tartaglia

Winslow

Goebel

et al. 1990

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The sequence TTTTTTT, 14 nt downstream of the L2R gene, is a subset of TTTTTNT which controls termination of transcription in VV early genes. The presence of VV-like early transcription termination sequences just downstream of early FPV genes has been described by others as well (Kumar & Boyle, 1990a, b;Binns et al, 1988).…”

Section: Discussionmentioning

confidence: 62%

Partial transcriptional mapping of the fowlpox virus genome and analysis of the EcoRI L fragment

Jl¹,

Jb³

et al. 1996

Journal of General Virology

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Several fowlpox virus (FPV) DNA fragments were selected by differential hybridization using cDNA of transcripts that were strongly transcribed early and/or late after infection of QT-35 cells. The EcoRI L fragment contained three strongly transcribed FPV genes: L1L, a late 1452 bp partial (amino end) ORF; L2R, an early/late 522 bp ORF; and L3R, a late 948 bp ORF. The protein products of L1L, L2R and L3R shared homology with the products ofvaccinia virus ( W ) genes H4L (RAP94), H5R (Ag35) and H6R (topoisomerase), respectively, suggesting a conservation of gene structure and order between VV and FPV. The 5' upstream non-coding sequences of L1L and L3R were A + T rich and the sequence 5' TAAATG 3' overlapped the predicted translation start codon. Primer extension analysis of the L2R transcript mapped the transcriptional start sites of early and late mRNAs 14 nt downstream of a VV early promoter-like critical region sequence, AAAATTGAA-AAAAAAA. A VV-like TAAAT late transcriptional element was present 20 nt upstream of the L2R ATG translational start codon. A plasmid with the putative early L2R promoter cloned upstream of the Newcastle disease virus haemagglutinin-neuraminidase (HN) cDNA as a reporter gene was at least 6-fold more effective in generating HN mRNA than plasmids containing the P7.5 or P11 VV promoters in transient expression assays in FPV-infected CEF cells treated with cytosine arabinoside. The L2R promoter was also able to express an amount of HN mRNA equal to that expressed by the W promoters late in infection.

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The Genome of Molluscum Contagiosum Virus: Analysis and Comparison with Other Poxviruses

et al. 1997

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Analysis of the molluscum contagiosum virus (MCV) genome revealed that it encodes approximately 182 proteins, 105 of which have direct counterparts in orthopoxviruses (OPV). The corresponding OPV proteins comprise those known to be essential for replication as well as many that are still uncharacterized, including 2 of less than 60 amino acids that had not been previously noted. The OPV proteins most highly conserved in MCV are involved in transcription; the least conserved include membrane glycoproteins. Twenty of the MCV proteins with OPV counterparts also have cellular homologs and additional MCV proteins have conserved functional motifs. Of the 77 predicted MCV proteins without OPV counterparts, 10 have similarity to other MCV proteins and/or distant similarity to proteins of other poxviruses and 16 have cellular homologs including some predicted to antagonize host defenses. Clustering poxvirus proteins by sequence similarity revealed 3 unique MCV gene families and 8 families that are conserved in MCV and OPV. Two unique families contain putative membrane receptors; the third includes 2 proteins, each containing 2 DED apoptosis signal transduction domains. Additional families with conserved patterns of cysteines and putative redox active centers were identified. Promoters, transcription termination signals, and DNA concatemer resolution sequences are highly conserved in MCV and OPV. Phylogenetic analysis suggested that MCV, OPV, and leporipoxviruses radiated from a common poxvirus ancestor after the divergence of avipoxviruses. Despite the acquisition of unique genes for host interactions and changes in GC content, the physical order and regulation of essential ancestral poxvirus genes have been largely conserved in MCV and OPV.

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Comparison of a Conserved Region in Fowlpox Virus and Vaccinia Virus Genomes and the Translocation of the Fowlpox Virus Thymidine Kinase Gene

Cited by 40 publications

References 25 publications

Nucleotide sequence analysis of a 10.5 kbp HindIII fragment of fowlpox virus: relatedness to the central portion of the vaccinia virus HindIII D region

Nucleotide sequence analysis of a 10.5 kbp HindIII fragment of fowlpox virus: relatedness to the central portion of the vaccinia virus HindIII D region

Partial transcriptional mapping of the fowlpox virus genome and analysis of the EcoRI L fragment

The Genome of Molluscum Contagiosum Virus: Analysis and Comparison with Other Poxviruses

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