Comparison of a New and Rapid Method: Brucella Coombs Gel Test With Other Diagnostic Tests

Kalem, Fatma; Ergün, Ayşe Gül; Durmaz, Süleyman; Doğan, Metin; Ertuğrul, Ömür; Gündem, Seval

doi:10.1002/jcla.21934

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…In our study, sensitivity of BCGT screening with 1/40 dilution of sera as suggested by the producer was found as 97%, while the specificity was 70.3%. Sensitivity rates of BCGT reported in other studies from Turkey (94%-100%) are similar to ours, while the specificity rate we found is lower than the reported 82%-100% rates (13)(14)(15)(16). İrvem et al (13) reported excellent agreement with AHG TAT and an immunocapture agglutination test.…”

Section: Discussionsupporting

confidence: 88%

“…Alternative methods, such as the enzyme-linked immunosorbent assay (ELISA) and AHG gel test, with which more samples in shorter time periods can be tested, are presented against standardization problems of agglutination tests with the possibility of different evaluations by different people in different laboratories, but evaluation of these tests is necessary before substitution (6)(7)(8)(13)(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of standard agglutination tests, enzyme immunoassay, and Coombs gel test used in laboratory diagnosis of human brucellosis

Koçman¹,

Erensoy²,

Taşbakan³

et al. 2018

Turk J Med Sci

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Background/aim: It was aimed to evaluate the results of Rose Bengal (RB), ELISA total tests (IgM and IgG), and the Brucella Coombs gel test (BCGT), which are used as screening tests, with the combined results of a tube agglutination test (standard Wright test: SWT) and a tube agglutination test with antihuman globulin (AHG TAT). Materials and methods:Samples from 97 patients prediagnosed with brucellosis (age ≥18 years) were screened with RB, ELISA, and BCGT. SWT < 160 samples and RB-negative but ELISA-or BCGT-positive samples were tested by AHG TAT. SWT ≥ 160 or AHG TAT ≥ 160 was taken as reference.Results: Thirty-two of 56 RB-positive samples and one RB-negative but ELISA-and BCGT-positive sample were found to be ≥160 with SWT or AHG TAT. Sensitivity, specificity, accuracy, and agreement (kappa) values according to SWT ≥ 160 or AHG TAT ≥ 160 positivity were as follows, respectively:

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Comparison of standard agglutination tests, enzyme immunoassay, and Coombs gel test used in laboratory diagnosis of human brucellosis

Koçman¹,

Erensoy²,

Taşbakan³

et al. 2018

Turk J Med Sci

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“…The sensitivity rates of BCGT compared to the Brucella immuncapture test reported in scientific studies from Turkey are between 94-100%, and the specificity rates are between 82-100% [10,21]. Kalem et al [22] performed a study that compared both ELISA and Coombs Gel tests in a similar fashion as in our study. They reported that BCGT and ELISA tests had high sensitivity (100% and 92.8%, respectively) and specificity (100% and 79.7%, respectively) when compared to the immuncapture test.…”

Section: Discussionsupporting

confidence: 65%

A comparison of a new diagnostic test of the human Brucellosis, the Brucella Coombs Gel Test, with other methods

Karameşe

Acar

2020

Acta Medica Alanya

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Amaç: Bu çalışmada, rutin laboratuvarlarda brusellozun tanısında kullanılan Brucella immuncapture test (BCAP), Rose Bengal (RB), Standart Tüp Aglütinasyon (STA) ve ELISA testleri ile yeni bir serolojik test olan Brucella Coombs Gel Test (BCGT)'in etkinliğinin karşılaştırılması amaçlanmıştır. Hastalar ve Yöntem: Dâhiliye, enfeksiyon hastalıkları ve pediatri kliniklerinden bruselloz ön tanısı ile laboratuvarımıza gönderilen 107 hastaya ait serum örneklerinde BCAP, RB, STA, ELISA IgG/IgM ve BCGT testleri uygulanmıştır. Cohen's Kappa testi ile tanı testleri arasındaki uyum istatistiksel olarak analiz edilmiştir. Ayrıca, BCGT testinin duyarlılık, özgüllük, pozitif ve negatif prediktif değerleri saptanmıştır. Bulgular: Elde edilen verilere göre; 107 hastanın 102'si (95.3%) BCAP testi ile 96'sı (%89.7) RB testi ile 80'i (%74.8) STA testi ile 100'ü (%93.5) BCGT ile 104'ü (%97.2) ELISA IgG testi ile ve 101'i (94.3%) ELISA IgM testi ile pozitif olarak tespit edilmiştir. Yapılan istatistiksel analizler sonucunda (Cohen's Kappa Test), BCAP ile BCGT (K=0.824) arasında güçlü uyum saptanmıştır. Ayrıca BCGT testinin duyarlılık ve özgüllüğü sırasıyla %98.08 ve %71.43 olarak saptanmıştır. Sonuç: BCGT insan brusellozunun tanısında kullanılabilecek umut vadeden bir teknik gibi görünen, hızlı, az maliyetli ve yüksek duyarlılığa sahip bir testtir. Ancak, bu testin rutin laboratuvarlarında kullanılabilirliğini destekleyecek daha fazla bilimsel çalışmaya ihtiyaç vardır.

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“…Values above cutoff were taken as positive. The sensitivity, specificity, positive predictive value (PPV) and, negative predictive value (NPV) of the test are 92.8, 79.7, 50, and 90%, respectively ( 15 ).…”

Section: Methodsmentioning

confidence: 99%

Occupational Exposure Assessment and Seroprevalence of Brucella Specific Antibodies Among Veterinarians in the Northern Palestine

2022

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Background: Brucellosis is one of the most popular zoonosis in the world caused by bacteria belonging to the genus Brucella. The disease is considered an occupational risk to persons dealing with animals and animal products. Brucellosis is endemic in livestock in Palestine. Yet, few studies investigated human brucellosis in Palestine. We aimed to estimate Brucella seropositivity among veterinary healthcare professionals in Northern Palestine, and to assess the associated risk factors.Methods: A cross-sectional study was conducted in four governorates in the Northern West Bank (Jenin, Nablus, Qalqylia, and Tulkarm). A sample of 100 veterinarians was collected. Participants were interviewed using a structured questionnaire to assess risk factors. Blood samples were collected to be screened for the presence of anti-Brucella IgG using the Enzyme-Linked Immunosorbent Assay (ELISA).Results: The seroprevalence of Brucellosis by ELISA was 76%. Risk factors included working in the public sector, dealing with animals' vaccination, longer period of exposure, and advancing in age.Conclusions: Brucellosis is a high-risk occupational disease among veterinarians. Its prevalence rate among veterinary healthcare workers in the Northern West Bank, Palestine was very high compared to neighboring countries and internationally.

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Comparison of a New and Rapid Method: Brucella Coombs Gel Test With Other Diagnostic Tests

Abstract: According to results BCGT can be used as a diagnostic test in routine laboratories after more comprehensive studies in control groups and patients.

Cited by 9 publications

References 15 publications

Comparison of standard agglutination tests, enzyme immunoassay, and Coombs gel test used in laboratory diagnosis of human brucellosis

Comparison of standard agglutination tests, enzyme immunoassay, and Coombs gel test used in laboratory diagnosis of human brucellosis

A comparison of a new diagnostic test of the human Brucellosis, the Brucella Coombs Gel Test, with other methods

Occupational Exposure Assessment and Seroprevalence of Brucella Specific Antibodies Among Veterinarians in the Northern Palestine

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