Comparison of a Novel Redox Dye Cell Growth Assay to the ATP Bioluminescence Assay

Squatrito, Robert C.; Buller, Richard E.

doi:10.1006/gyno.1995.1190

Cited by 40 publications

(18 citation statements)

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“…This is accordant with other results for Swiss 3T3 fibroblast cells [19] and epithelial ovarian carcinoma cells [20]. Apart from the sensitivity differences of cell types against resazurin, experiment protocols varying in the concentration and the incubation time have to be considered.…”

Section: Discussionsupporting

confidence: 59%

Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay

Xiao

Zhang

Wang

et al. 2010

Appl Biochem Biotechnol

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Cell microencapsulation is a promising approach for cell implantation, cell-based gene therapy and large-scale cell culture. For better quality control, it is important to accurately measure the microencapsulated cell viability and proliferation in the culture. A number of assays have been used for this purpose, but limitations arise. In this study, we investigated the feasibility and reliability of resazurin as a cell growth indicator in microencapsulated culture system. According to the experiment data, there was a reversible, time- and dose-dependent growth inhibition as observed for resazurin application in encapsulated cells. A positive relationship was observed between reduction of resazurin and CHO cell number in microcapsule. Moreover, the resazurin assay provided an equivalent result to the commonly used MTT method in determining CHO cell proliferation in APA microcapsule with no notable influence on cell distribution and organization pattern. In conclusion, resazurin assay is offered as a simple, rapid and non-invasive method for in vitro microencapsulated cell viability and proliferation measurement.

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Section: Discussionsupporting

confidence: 59%

Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay

Xiao

Zhang

Wang

et al. 2010

Appl Biochem Biotechnol

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“…Each cell line has unique metabolic properties and must be individually characterized to determine the experimental parameters (for example, incubation time and dye dilution) for optimal conversion from the oxidized to the reduced form of Alamar Blue [15,16] . Squatrito et al [17] have shown that the Alamar Blue assay correlates poorly with the ATP assay and exerts marked cytotoxicity which was time and dose dependent. The significant cytotoxicity seen as a result of prolonged exposure to the Alamar dye essentially eliminates the possibility of accurate multiple time point readings over a span of several days [17] .…”

Section: Measurement Of Biocompatibility and Cytotoxicitymentioning

confidence: 99%

“…Squatrito et al [17] have shown that the Alamar Blue assay correlates poorly with the ATP assay and exerts marked cytotoxicity which was time and dose dependent. The significant cytotoxicity seen as a result of prolonged exposure to the Alamar dye essentially eliminates the possibility of accurate multiple time point readings over a span of several days [17] . Therefore, the assay should only be used as a rapid preliminary screening assay.…”

Section: Measurement Of Biocompatibility and Cytotoxicitymentioning

confidence: 99%

Evaluation of Biocompatibility and Cytotoxicity Using Keratinocyte and Fibroblast Cultures

Wiegand¹,

Hipler²

2009

Skin Pharmacol Physiol

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Determination of biocompatibility and cytotoxicity is part of the initial evaluation of medical devices stipulated by ISO standards on biological evaluation of medical devices. Cell culture systems for testing biological reactions to drugs, biomaterials or treatment techniques used in various disciplines have been gaining importance. A wide variety of cell lines are commonly used: cultured fibroblasts from human skin, buccal mucosa, periodontal membrane or embryonic lung; epithelial and HeLa cells; cultures of human keratinocytes and HaCaT cells; different murine cell lines (C3H-L, Balb/c 3T3, L929 and others) as well as murine cells cultured from liver and spleen; T lymphocytes from lymph nodes and macrophages obtained by lavage. All these cells are suitable for the use in biocompatibility tests. Nevertheless, the general opinion is that cytotoxicity tests in vitro will be more convincing when performed with cells that are homologous with the human tissue concerned. In accordance, appropriate cell lines for the use in cytotoxicity and tolerance tests concerning the skin would be human dermal fibroblasts and human epidermal keratinocytes, as they take an active part in the immune response, inflammatory processes and wound healing.

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“…Assays were performed before and after treatment for assessing baseline fluorescence and the effect of drug on cell proliferation. Resazurin has been reported to be cytotoxic at high concentrations and exposure beyond 24 hours [12]. Since cell lines respond differently to resazurin, [13], we conducted a static assay to establish that 5% (v/v) resazurin could be used over at least a 24 hour period without toxicity to the MCF-7 cell line.…”

Section: Resazurin Assaymentioning

confidence: 99%

Pharmacokinetic/Pharmacodynamic (PK/PD) Modeling of Anti-Neoplastic Agents

Lexcen¹,

Salem²,

Elkhatib³

et al. 2012

Readings in Advanced Pharmacokinetics - Theory, Methods and Applications

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Comparison of a Novel Redox Dye Cell Growth Assay to the ATP Bioluminescence Assay

Cited by 40 publications

References 0 publications

Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay

Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay

Evaluation of Biocompatibility and Cytotoxicity Using Keratinocyte and Fibroblast Cultures

Pharmacokinetic/Pharmacodynamic (PK/PD) Modeling of Anti-Neoplastic Agents

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