Comparison of a slide coagglutination technique with the Minitek system for confirmation of Neisseria gonorrhoeae

Hampton, Kenneth D.; Stallings, Richard A.; Wasilauskas, Benedictl .

doi:10.1128/jcm.10.3.290-292.1979

Cited by 14 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We did not observe the cross-reactions with N. sicca or Moraxella sp. reported with the older test (12). Subsequent serological analysis of crossreacting strains in this laboratory with specific monoclonal reagents for N. gonorrhoeae indicated that they belonged to serovar Ae (i.e., reacted with reagent 4G5), one of the pooled monoclonal reagents used in the test.…”

Section: Discussionmentioning

confidence: 89%

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

1988

View full text Add to dashboard Cite

The performance of eight methods in identifying Neisseria species, particularly N. gonorrhoeae, was evaluated. These methods included four rapid carbohydrate utilization tests (Gonobio-Test, Neisseria-Kwik, RIM-N, and Minitek); the Gonochek 11, a test which is based on the utilization of chromogenic substrates; and three monoclonal antibody tests (Syva MicroTrak, GonoGen, and Phadebact Monoclonal GC OMNI Test). In all, 182 isolates comprised in six species of Neisseria as well as Branhamella catarrhalis and Moraxella sp. were tested. Cystine-tryptic digest agar supplemented with sugars was included for reference purposes. In the carbohydrate utilization tests, the sensitivity and specificity of the Neisseria-Kwik and Minitek tests for the identification of N. gonorrhoeae were 100%. This compared with sensitivities and specificities, respectively, of 100 and 99.1% for the Gonobio-Test and 99.1 and 100% for cystine-tryptic digest agar sugars and the RIM-N test. The sensitivity and specificity of the Gonochek II test were 99.0 and 86.7%, respectively. Although most test kits did not claim to identify all Neisseria species, in several cases isolates of N. subflava were misidentified or could be misinterpreted as N. gonorrhoeae or N. meningitidis. With the monoclonal reagents, the Syva MicroTrak system was 100% sensitive and 100% specific. The GonoGen test was both 99.1% sensitive and specific, while the Phadebact Monoclonal GC OMNI Test was 99.1% sensitive but 91.2% specific. With this latter test, cross-reactions were observed with strains of B. catarrhalis, N. cinerea, and N. lactamica.

show abstract

Section: Discussionmentioning

confidence: 89%

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

1988

View full text Add to dashboard Cite

show abstract

“…cinerea strains were not included in early evaluations of the BACTEC system or other Neisseria identification methods that are based on radiometric or spectrophotometric detection of CO2 produced from sugars (9, 28). Similarly, published reports dealing with tests based on rapid carbohydrate utilization (2, 3, 7, 13, 15, 17, 21-25, 27, 28, 32-35), detection of enzymes such as PRO and gammaglutamylaminopeptidase (10,13,26,32,33), or coagglutination (1,3,4,8,11,15,20,24,26) have not described any results for N. cinerea.…”

Section: In Thementioning

confidence: 99%

Difficulties in differentiating Neisseria cinerea from Neisseria gonorrhoeae in rapid systems used for identifying pathogenic Neisseria species

Boyce¹,

Mitchell²

1985

J Clin Microbiol

View full text Add to dashboard Cite

Neisseria cinerea and Neisseria gonorrhoeae may occur at the same body sites and may have similar colony morphologies. Ideally, systems used for rapid identification of N. gonorrhoeae should be able to differentiate N. cinerea from gonococci. We tested seven N. cinerea strains using the Gonochek II (Du Pont Diagnostics), Minitek (BBL Microbiology Systems), RapID-NH (Innovative Diagnostics, Inc.), RIM-N (American Microscan), and Phadebact (Pharmacia Diagnostics) systems. We found that the reactions produced by N. cinerea in Gonochek II, Minitek, and RapID-NH kits could be confused with the results produced by some strains of N. gonorrhoeae. The susceptibility of N. cinerea to colistin, its ability to grow on tryptic soy or Mueller-Hinton agar, and its inability to grow on modified Thayer-Martin medium help differentiate it from gonococci.

show abstract

“…However, there have been reports of crossreactivity between gonococci and other members of Neisseria (20,22). Agglutination and coagglutination methods are also prone to cross- reactivity among the species and thus warrant further evaluations and improvements (3,9,10,13). Other methods used for the identification of Neisseria spp.…”

Section: Resultsmentioning

confidence: 99%

Clinical evaluation of the rapid carbohydrate degradation microtube method for identification of Neisseria species

Qadri¹,

Parker²

1981

J Clin Microbiol

View full text Add to dashboard Cite

The rapid carbohydrate degradation (Carr Microbiologicals Wichita, Kans.) microtube method is a new test system designed for the identification of Neisseria spp. The system consists of four microtubes containing different carbohydrates in a peptone-Bitone basal medium. This method was evaluated for accuracy and speed in identifying species of Neisseria. Of the 386 clinical isolates used in this study, 98.4% were correctly identified to species level in 4 h with the rapid carbohydrate degradation system; parallel testing of the same isolates with conventional cystine-tryptic agar resulted in 96.1% accuracy in 48 h.

show abstract

Comparison of a slide coagglutination technique with the Minitek system for confirmation of Neisseria gonorrhoeae

Cited by 14 publications

References 13 publications

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test

Difficulties in differentiating Neisseria cinerea from Neisseria gonorrhoeae in rapid systems used for identifying pathogenic Neisseria species

Clinical evaluation of the rapid carbohydrate degradation microtube method for identification of Neisseria species

Contact Info

Product

Resources

About