Comparison of a three-peptide separation by capillary electrochromatography, voltage-assisted liquid chromatography and nano-high-performance liquid chromatography

Szucs, Veronika; Freitag, Ruth

doi:10.1016/j.chroma.2004.05.103

Cited by 25 publications

(16 citation statements)

References 35 publications

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“…Therefore, we assume that a low EOF was presumably generated by the free silanol groups of the short outlet part of the capillary. These findings are in accordance with the published results obtained with hydrophobic monoliths [19,20] and are underlining that voltageassisted CLC is the dominant separation technique in the case of RP ROMP monoliths.…”

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Voltage‐assisted capillary LC of peptides using monolithic capillary columns prepared by ring‐opening metathesis polymerization

et al. 2007

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We examined the use of monolithic capillary columns prepared via ring-opening metathesis polymerization (ROMP) for peptide separation in voltage-assisted capillary LC (voltage-assisted CLC). In order to demonstrate their potential for peptide separation, ROMP-derived monoliths with RP properties were prepared. The preparation procedure of monoliths was transferred from ROMP monoliths optimized for CLC. ROMP monoliths were synthesized within the confines of 200 microm id fused-silica capillaries with a length of 37 cm. After optimization of the chromatographic conditions, the separation performance was tested using a well-defined set of artificial peptides as well as two peptidic mixtures resulting from a tryptic digest of BSA as well as a collagenase digest of collagen. ROMP monoliths showed comparable performance to other monolithic separation media in voltage-assisted CLC published so far. Therefore, we conclude that by optimizing the composition of the ROMP monoliths as well as by using the controlled manner of their functionalization, ROMP monoliths bear a great potential in CLC and CEC.

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confidence: 95%

“…The RP ROMP monoliths used for our experiments did not bear charges and are therefore comparable to the neutral "C1"-type column described by Szucs and Freitag [20]. Thus, in order to evaluate the applicability of ROMP monoliths for voltage-assisted CLC, we used an approach similar to the published method.…”

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Voltage‐assisted capillary LC of peptides using monolithic capillary columns prepared by ring‐opening metathesis polymerization

et al. 2007

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“…More recently, it was reported that Angelicae dahuricae radix suppressed the development of atopic dermatitis-like skin lesions and could be used for the treatment of hypertension and asthma (3,7,8). A number of analytical methods have been reported for the determination of several coumarins in Angelicae dahuricae radix, including capillary electrochromatography, high-performance liquid chromatography, liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry and high-speed countercurrent chromatography (7,(9)(10)(11)(12). It appears that the crude extract and essential oils of Angelicae dahuricae radix may include multiple potentially active chemical compounds.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

Jeong

Kim

Lee

et al. 2012

Molecular Medicine Reports

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“…A mixed mode retention mechanism based primarily on electrostatic interactions modified by hydrophilic ones seemed to be most suited to interpret the behavior of the analytes. Another monolithic stationary phase, composed of poly(glycidyl methacrylate-coethylene dimethylacrylate), was used in the comparative study of three separation modes, CEC, capillary LC (CLC, also called nanoLC), and voltage-assisted CLC (in other words termed pCEC) [168]. The stationary phase could be directly used as neutral C1-type column for CLC and voltage-assisted CLC, whereas its application in CEC became possible after derivatization of the epoxy groups with ionogenic N-ethylbutylamine moiety, which ensured high EOF flow rate.…”

Section: Electrochromatographymentioning

confidence: 99%

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

2006

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The article gives a comprehensive review on the recent developments in the applications of high-performance capillary electromigration methods, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides. The article presents new approaches to the theoretical description and experimental verification of electromigration behavior of peptides, covers the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample preparation, suppression of peptide adsorption, new developments in individual separation modes, and new designs of detection systems. Several types of applications of capillary electromigration methods to peptide analysis are presented: conventional qualitative and quantitative analysis, purity control, determination in biomatrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid and sequence analysis, and peptide mapping of proteins. Some examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Comparison of a three-peptide separation by capillary electrochromatography, voltage-assisted liquid chromatography and nano-high-performance liquid chromatography

Cited by 25 publications

References 35 publications

Voltage‐assisted capillary LC of peptides using monolithic capillary columns prepared by ring‐opening metathesis polymerization

Voltage‐assisted capillary LC of peptides using monolithic capillary columns prepared by ring‐opening metathesis polymerization

Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

Recent developments in capillary electrophoresis and capillary electrochromatography of peptides

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