Comparison of a toxin neutralization assay and ELISA for determination of pertussis toxin antibodies

Isacson, Jerker; Trollfors, Birger; Lagergrd, Teresa; Taranger, J

doi:10.1016/s0888-0786(96)01072-4

Cited by 6 publications

(6 citation statements)

References 20 publications

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“…However, antibody titres obtained by these two assays display a linear correlation. This correlation has previously been shown for pertussis toxin antibodies induced by acellular pertussis vaccination ( 2 , 13 , 14 , 17 , 21 ), by whole-cell pertussis vaccination ( 14 ), by Bordetella pertussis infections ( 16 ) and in general ( 10 , 18 ).…”

Section: Discussionsupporting

confidence: 76%

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Pertussis serology: assessment of IgG anti‐PT ELISA for replacement of the CHO cell assay*

Dalby¹,

Sørensen

Petersen

et al. 2010

APMIS

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Dalby T, Sørensen C, Petersen JW, Krogfelt KA. Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay. APMIS 2010; 118: 968–72.Two types of serological assays are commonly used for the assessment of pertussis vaccine-induced antibodies; the Chinese hamster ovary cell (CHO cell) assay and the immunoglobulin G (IgG) anti pertussis toxin (PT) enzyme-linked immunosorbent assay (IgG anti-PT ELISA). Recently, both the techniques have been modified to improve performance with sera with interfering activity (CHO cell assay) or with heat-treated sera (IgG anti-PT ELISA). These two improved techniques were compared by the analysis of 100 individual serum samples from a previous clinical trial and 213 sera from a longitudinal serum collection from 20 Danish adults recently vaccinated with the Danish acellular pertussis vaccine. The comparison showed a significant linear correlation between the results of the two assays with a p-value of <0.0001 for the 100 individual samples. We, therefore, conclude that the improved IgG anti-PT ELISA can be used as a replacement for the often troublesome and time-consuming CHO cell assay for the measurement of vaccine-induced human antibodies to PT.

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Section: Discussionsupporting

confidence: 76%

“…Previous studies have shown a good correlation between the IgG anti-PT ELISA and the CHO cell assay (2,10,(13)(14)(15)(16)(17)(18). However, both the IgG anti-PT ELISA and the CHO cell assay have been altered recently.…”

mentioning

confidence: 96%

Pertussis serology: assessment of IgG anti‐PT ELISA for replacement of the CHO cell assay*

Dalby¹,

Sørensen

Petersen

et al. 2010

APMIS

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“…A clinical study involving 192 children showed a correlation between antibody binding titers obtained by ELISA and the toxin neutralization activity [30], however this study also showed that an antibody titer obtained by one method could generally not be used to predict a titer for the other method with accuracy at the level of an individual serum sample. Therefore, due to the importance of anti-toxin activity and the relatively small number of animals in this non-human primate (NHP) study, toxin neutralizing activity was assessed in addition to assessing the anti-PT antibody binding titers (Figure 5A).…”

Section: Compared To Ap Vaccine Primed Baboons Wp Vaccine Primed Baboons Had Higher Pt-neutralizing Titers That Were Sustained Over 14 Momentioning

confidence: 64%

Immunological Distinctions between Acellular and Whole-Cell Pertussis Immunizations of Baboons Persist for at Least One Year after Acellular Vaccine Boosting

et al. 2020

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While both whole-cell (wP) and acellular pertussis (aP) vaccines have been highly effective at reducing the global pertussis disease burden, there are concerns that compared to wP vaccination, the immune responses to aP vaccination may wane more rapidly. To gain insights into the vaccine elicited immune responses, pre-adult baboons were immunized with either aP or wP vaccines, boosted with an aP vaccine, and observed over a nearly two-year period. Priming with a wP vaccine elicited a more Th17-biased response than priming with aP, whereas priming with an aP vaccine led to a more Th2-biased response than priming with wP. These differences were maintained after aP vaccine boost immunizations. Compared to aP, animals primed with a wP vaccine exhibited greater numbers of pertussis specific memory B cells. While aP and wP vaccine priming initially elicited similar levels of anti-pertussis toxin antibody, titers declined more rapidly in aP vaccine primed animals leading to a 4-fold difference. Both wP and aP vaccine immunization could induce serum bactericidal activity (SBA); however, only one wP vaccine immunization was required to elicit SBA while multiple aP vaccine immunizations were required to elicit lower, less durable SBA titers. In conclusion, when compared to aP vaccine, priming with wP vaccine elicits distinct cellular and humoral immune responses that persist after aP vaccine boosting.

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“…Therefore, the authors speculated that the ELISA, CHO, and PBMC assays measure distinct fractions of anti-PT antibodies [ 56 ]. In contrast, the majority of CHO-cell-based assays have shown a clear correlation (r > 0.80) between the concentration of anti-PT IgG antibodies and CHO cell neutralizing titers [ 46 , 47 , 48 , 49 , 55 , 57 , 58 , 59 ], concluding that basic ELISA measurements could be used to demonstrate the neutralization capacity of antibodies [ 48 , 57 , 59 ]. Furthermore, most of the neutralization and protection is based on anti-PT IgG antibodies, whereas IgA and IgM play a minor role [ 59 ].…”

Section: The Role Of Neutralizing Antibodies To Pertussis Toxin (Ptnas)mentioning

confidence: 99%

“…In the light of different studies performed, PTNAs do correlate with the total amount of anti-PT IgG antibodies and are induced considerably both after infection and vaccination, but they also indicate that high antibody concentration does not always guarantee good neutralization capacity [ 29 , 47 , 55 , 57 ]. Although these studies increase our understanding of PT antibody-based protection, further modifications for future testing might be needed to better show the actual fraction of neutralizing antibodies after vaccination or natural infection.…”

Section: The Role Of Neutralizing Antibodies To Pertussis Toxin (Ptnas)mentioning

confidence: 99%

Evaluation of Anti-PT Antibody Response after Pertussis Vaccination and Infection: The Importance of Both Quantity and Quality

Barkoff

Knuutila

Mertsola

et al. 2021

Toxins

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Pertussis toxin (PT) is considered the main virulence factor causing whooping cough or pertussis. The protein is widely studied and its composition was revealed and sequenced already during the 1980s. The human immune system creates a good response against PT when measured in quantity. However, the serum anti-PT antibodies wane rapidly, and only a small amount of these antibodies are found a few years after vaccination/infection. Therefore, multiple approaches to study the functionality (quality) of these antibodies, e.g., avidity, neutralizing capacity, and epitope specificity, have been investigated. In addition, the long-term B cell memory (Bmem) to PT is crucial for good protection throughout life. In this review, we summarize the findings from functional PT antibody and Bmem studies. These results are discussed in line with the quantity of serum anti-PT antibodies. PT neutralizing antibodies and anti-PT antibodies with proper avidity are crucial for good protection against the disease, and certain epitopes have been identified to have multiple functions in the protection. Although PT-specific Bmem responses are detectable at least five years after vaccination, long-term surveillance is lacking. Variation of the natural boosting of circulating Bordetella pertussis in communities is an important confounding factor in these memory studies.

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Comparison of a toxin neutralization assay and ELISA for determination of pertussis toxin antibodies

Cited by 6 publications

References 20 publications

Pertussis serology: assessment of IgG anti‐PT ELISA for replacement of the CHO cell assay*

Pertussis serology: assessment of IgG anti‐PT ELISA for replacement of the CHO cell assay*

Immunological Distinctions between Acellular and Whole-Cell Pertussis Immunizations of Baboons Persist for at Least One Year after Acellular Vaccine Boosting

Evaluation of Anti-PT Antibody Response after Pertussis Vaccination and Infection: The Importance of Both Quantity and Quality

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