Comparison of ABO isoagglutinin titres by three different methods: tube haemagglutination, micro‐column agglutination and automated immunohematology analyzer based on erythrocyte‐magnetized technology

Shim, Hyoeun; Hwang, Joo Hyung; Kang, Su Jin; Seo, Hee Seoung; Park, Eun Young; Park, Kyoung Un; Kong, Sun Young

doi:10.1111/vox.12878

Cited by 17 publications

(20 citation statements)

References 25 publications

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“…Even on comparison of card method with TT and EMT, agreement results were comparable in case of IgM but low in IgG. 17 Taking all BGs together, our study gave more than 90% results of IgG as well as IgM within one titer difference.…”

Section: Discussionmentioning

confidence: 50%

Evaluation of Erythrocytes Magnetized Technology for Measurement of ABO Isoagglutinin Titers

Chopra

Bhardwaj²,

Samkaria³

et al. 2021

J Lab Physicians

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Background A variation in the measurement of ABO antibody titer has been seen among different laboratories due to lack of standardization. In our study, we aim to evaluate automated ABO isoagglutinin titer measurements by erythrocytes magnetized technology (EMT) and compare with conventional tube technique (TT). Methods We performed ABO isoagglutinin titration on samples received in a reference laboratory during a period of 2 months. A total of 134 tests for immunoglobulin G (IgG) titer and 116 for immunoglobulin M (IgM) for anti-A or anti-B were included in the study. Samples were processed for ABO isoagglutination titers by both TT and EMT by QWALYS-3 (Diagast, France). Microsoft Excel was used to compile data, for all calculations, and to draw graphs and plots. The number and percentage of cases within ±1, ±2, or ±3 titer difference (TT-EMT) were calculated. Results Median titers and their ranges obtained by EMT were higher or equal to those by TT for all IgM and IgG ABO-antibodies in all blood group (BGs), except anti-A IgM in (BG) O that was lower by EMT (32 [4:128]) than TT (48 [8:256]). One twenty one (121/134, 90.3%) cases of IgG titer showed an agreement by both methods (within ± one titer difference). One hundred seven cases (107/116, 92.2%) for IgM titer were within one titer difference by both the methods. Conclusion Results of titration by EMT-based automated instrument QWALYS-3 and conventional TT may vary by one titer dilution in the majority of cases. Use of consistent method for patient management is, therefore, advised.

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Section: Discussionmentioning

confidence: 50%

Evaluation of Erythrocytes Magnetized Technology for Measurement of ABO Isoagglutinin Titers

Chopra

Bhardwaj²,

Samkaria³

et al. 2021

J Lab Physicians

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“…Shim et al. [38] compared three methods of antibody titration using 40 samples and found that the median IgM and IgG titres were higher by CAT with the a better agreement for IgM than for IgG. In the present study, median titres were determined separately for IgM and IgG for anti‐A and anti‐B as illustrated in Fig.…”

Section: Discussionmentioning

confidence: 61%

A prospective, observational study to compare effect of DTT treatment and heat inactivation on ABO isoagglutinin titers in O blood group donors

Pandey

Setya

Ranjan

et al. 2021

ISBT Science Series

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Background and aims In order to determine actual concentration of clinically significant IgG antibodies, there is a need to inactivate IgM antibodies, which can be performed by DTT or heat. The aim of the present study was to compare the effect of DTT treatment and heat inactivation (HI) on ABO isoagglutinin titres performed by column agglutination technology (CAT) and conventional tube technique (CTT). Materials and methods This was a prospective, observational study conducted from October 2018 to March 2020. All consecutive O group donors were included. Serum from each donor was treated with DTT, and heat and tests were performed by CTT and CAT before and after treatment. Results A total of 2005 donors were included. 401 (20%) samples had inter‐observer variation by CTT, and 69 (3·4%) had inter‐observer variation by CAT. Median anti‐A and anti‐B titre results were higher with CAT as compared to CTT. Median anti‐A and anti‐B IgG titre results showed a one‐ to twofold difference between CTT and CAT with higher titres reported by CAT. Median IgM titres decreased two‐ to threefold after DTT treatment, whereas onefold decrease was confirmed after HI. Concordance was found to be poor. However, correlation was found to be strong. Conclusion There is overestimation of ABO IgG antibody titres and hence, estimation of ABO IgG titres after inactivation of IgM antibodies is strongly recommended. DTT treatment was found to be superior to HI in terms of efficiency in elimination of IgM activity.

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“…Similarly, in the present study, the correlation was found to be strong or very strong between CTT (1+) and CAT (1+, 2+) for both IgG and IgM of both anti-A and anti-B isoagglutinins. Shim et al compared three methods of antibody titration using 40 samples and found that median ABO IgM and IgG titres of all blood groups obtained by the erythrocytemagnetized technology method were higher than those obtained by the conventional tube haemagglutination and micro-column agglutination [36]. They found that the agreement between the methods was comparable in case of IgM but low in case of IgG.…”

Section: Discussionmentioning

confidence: 99%

Comparison of ABO isoagglutinin titres by three different methods in group O blood donors

Pandey

Setya

Ranjan

et al. 2021

ISBT Science Series

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Background and aims Antibody titration by the manual method has certain limitations. The aim of this study was to compare the manual conventional test tube technique (CTT) with semi-automated, column agglutination technique (CAT) and fully automated haemagglutination/solid-phase red cell adherence (HA/SPRCA) technology in measuring antibody titres of anti-A and anti-B isoagglutinins of blood group O blood donors. The median IgM and IgG antibody titres with 1+, 2+ and 3+ end-points were determined for each technology, and the agreement and correlation between results of the 3 technologies were evaluated.Materials and methods This was a prospective, observational study conducted from October 2018 to March 2020. All consecutive O group blood donors, who donated blood at the Department of Transfusion Medicine at a tertiary healthcare centre, were included in the study. All samples were consecutively tested by CTT, CAT and HA/SPRCA, and the results were recorded and compared.Results A total of 2005 donors were included. Inter-observer variation was observed in 295 (14.71%) samples by the CTT and in 51 (2.54%) by the CAT. There was 43.4% and 40.3% agreement of anti-A IgM and IgG titres between CAT (2+ strength) and CTT (1+ strength), respectively. The agreement for anti-B IgM and IgG titres between CAT (2+ strength) and CTT (1+ strength) was 43.6% and 32%, respectively. While a strong correlation was found between CTT (1+ strength) and CAT (1+, 2+ strengths) for both IgM and IgG measurements of anti-A and anti-B isoagglutinins, the correlation between CTT (1+ strength) and HA/SPRCA (1+, 2+ strengths) was found to be weak. The median IgM titres for anti-A and anti-B were lower than the median IgG titres. ConclusionThe antibody titration levels were higher with CAT than with CTT, and those of HA/SPRCA were lower than those of CTT. Transfusion services need to decide which method would provide higher benefit to their patient population.

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Comparison of ABO isoagglutinin titres by three different methods: tube haemagglutination, micro‐column agglutination and automated immunohematology analyzer based on erythrocyte‐magnetized technology

Cited by 17 publications

References 25 publications

Evaluation of Erythrocytes Magnetized Technology for Measurement of ABO Isoagglutinin Titers

Evaluation of Erythrocytes Magnetized Technology for Measurement of ABO Isoagglutinin Titers

A prospective, observational study to compare effect of DTT treatment and heat inactivation on ABO isoagglutinin titers in O blood group donors

Comparison of ABO isoagglutinin titres by three different methods in group O blood donors

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