Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods

Floegel, Anna; Kim, Dae‐Ok; Chung, Sang‐Jin; Koo, Sung I.; Chun, Ock K.

doi:10.1016/j.jfca.2011.01.008

Cited by 1,342 publications

(983 citation statements)

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“…Floegel et al (2011) evaluated the antioxidant potential of 18 fruits, 13 vegetables, and 19 beverages consumed in the united States and verified that the antioxidant activity detected by the ABTS method was substantially higher when compared to those of the DPPH assay. According to these authors, the hydrophilic antioxidants with high pigmentation were better measured by the ABTS assay, indicating that this method can be more useful than the DPPH assay.…”

Section: Total Polyphenol Content and Antioxidant Activity Of Murtillmentioning

confidence: 85%

Phenolic compounds and antioxidant activity of hydroalcoholic extracts of wild and cultivated murtilla (Ugni molinae Turcz.)

Augusto¹,

Scheuermann²,

Alencar³

et al. 2014

Food Sci. Technol (Campinas)

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Over the last decade a considerable increase in the number of studies addressing the use of antioxidants from natural sources has led to the identification and understanding of the potential mechanisms of biologically active components. This results from the fact that they can be used to replace synthetic antioxidants commonly used in food. Murtilla (Ugni molinae Turcz) is a native berry grown in Chile, and in the present study, the phenolic composition and antioxidant activity of its fruits were studied. Hydroalcoholic extracts of dehydrated fruits from two genotypes of murtilla (Ugni molinae Turcz.) were produced. Extracts of wild murtilla and 14-4 genotype fruits had 19.35 and 40.28mg GAE/g for Total Phenolic Compounds, 76.48, and 134.35μmol TEAC/g for DPPH, and 157.04 and 293.99 μmol TEAC/g for ABTS, respectively. Components such as quercetin, epicatechin, and gallic, benzoic and hydrocaffeic acids were identified by CG/MS analysis. All of them showed antioxidant activity. Therefore, it is possible to say that the hydroalcoholic extracts of murtilla have antioxidant potential to be used in lipidic food.

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Section: Total Polyphenol Content and Antioxidant Activity Of Murtillmentioning

confidence: 85%

Phenolic compounds and antioxidant activity of hydroalcoholic extracts of wild and cultivated murtilla (Ugni molinae Turcz.)

Augusto¹,

Scheuermann²,

Alencar³

et al. 2014

Food Sci. Technol (Campinas)

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show abstract

“…Determination of antioxidant activity of fruits and vegetables is therefore important and there are several chemical methods of assessing antioxidant activity. The DPPH (2, 2-Diphenyl-1-picrylhydrazyl) and ABTS (2, 2 -Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging assays are popular because these methods are simple, inexpensive and widely used to determine total antiox-IJFS October 2013 Volume 2 pages 224-237 idant activities in fruits and vegetables (Floegel, Kim, Chung, Koo, & Chun, 2011). In the DPPH method, the scavenging of the stable DPPH radical by an antioxidant is measured colorimetrically.…”

Section: Introductionmentioning

confidence: 99%

Effect of drying techniques on the retention of antioxidant activities of Saskatoon berries

2013

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The main objective of this research was to compare the retention of antioxidant activity and total anthocyanin content of Saskatoon berries dried by freeze drying, microwave-vacuum drying, thin layer hot air drying and vacuum drying. Antioxidant activity of berry samples was determined by DPPH radical scavenging and ABTS radical scavenging, and the pH differential method was used to determine total anthocyanin content of the berry samples. The results showed that the freeze dried Saskatoon berries exhibited the highest retention of anthocyanin and antioxidant activity among the dried samples, followed by microwave-vacuum dried berries, thin layer hot air dried berries and vacuum dried berries. There were significant differences between the berry samples at P<0.05. DPPH radical scavenging and ABTS radical scavenging were correlated linearly with an R 2 value of 0.99 at P<0.05 showing their effectiveness for the determination of the antioxidant activity of the Saskatoon berries. However, the DPPH radical scavenging assay was more effective than the ABTS radical scavenging assay. The results also showed that antioxidant activity of the berries was highly correlated with the total anthocyanin content of the fruit. The reduction of anthocyanin in dried berry samples was linearly correlated with the reduction of DPPH radical scavenging with an R 2 value of 0.97 at P<0.05 and, also, linearly correlated with the reduction of ABTS radical scavenging with an R 2 value of 0.88 at P<0.05.

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“…This result was expected considering the "multimistura"'s phenolic and carotenoid content. The antioxidant capacity is similar to green tea, in which 1.85 mg Eq Ascorbic acid /g was reported after ABTS quantification, which ranks green tea in eighth position in a list of ten foods recognized as having the highest known antioxidant capacity 59 . Therefore, including the "multimistura" in the diet brings additional health benefits beyond its main purpose in fighting nutritional deficiency.…”

Section: Composição Em Micronutrientesmentioning

confidence: 57%

Characterization of a plant dietary supplement (“multimistura”) capacities, used in Mozambique to fight nutritional insecurity

Neto

Moldão‐Martins

Goulão

2016

BBR

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Biomed Biopharm Res. , 2016; (13) 2: , 155-179 155 Biomedical and Biopharmaceutical Research J o r n a l d e I n v e s t i g a ç ã o B i o m é d i c a e B i o f a r m a c ê u t i c a Characterization of a plant dietary supplement ("multimistura") capacities, used inMozambique to fight nutritional insecurity AbstractThe use of food dietary supplements, commonly called "multimisturas", has been adopted, in developing countries, as a strategy to combat micronutrient deficiency in the population.. The goal of this work was to study the composition and nutritional potential of a "multimistura" that was developed and used in a vulnerable community of Nampula, Mozambique to fight micronutrient deficiency in pregnant or lactating women and in children up to 8 years old. The micronutrient composition of the "multimistura" developed with locally available products did not show statistically significant differences when compared to commercial food supplements used in industrialized countries. Taking in account the composition results and the nutritional requirements of each target group, it was observed that the currently recommended daily intake should be, at least, doubled to fulfil the nutritional needs of each of the groups. The exception regards Children in the 1-3 year old group, would be an exception, as the doubling of ingestion would be detrimental since it would exceed the tolerable daily intake of magnesium. This work allowed the suggestion of some procedures aimed at balancing the supplement nutritional composition to leverage adequate levels of household nutritional security in the target community.Keywords: dietary supplement, food security, hidden hunger, Mozambique, multimistura. ResumoEm países em desenvolvimento, uma das formas de combate à carência de micronutrientes da população é o recurso a suplementos alimentares, comummente chamados de multimisturas, definidas como o produto obtido através da secagem, torrefação, moagem e mistura de ingredientes de origem vegetal. Neste trabalho pretendeu-se estudar a composição e adequabilidade de uma dessas multimisturas, desenvolvida numa comunidade vulnerável em Nampula,Moçambique, e utilizada no combate da carência de micronutrientes em grávidas, lactantes e crianças até aos 8 anos. A composição de micronutrientes da multimistura, desenvolvida com base em produtos locais, não se mostrou estatisticamente diferente da descrita em suplementos alimentares comerciais usados em países industrializados. Com base nas determinações efetuadas e nas necessidades nutricionais de cada grupo etário, constatou-se que as doses diárias recomendadas atualmente são nutricionalmente insuficientes sendo necessário, no mínimo, duplicar a quantidade de multimistura ingerida por cada um dos grupos. A exceção é o grupo das crianças dos 1 aos 3 anos onde a duplicação da dose seria prejudicial, pois implicaria que a dose diária aceitável de magnésio fosse ultrapassada. São sugeridas ainda algumas medidas para otimizar a composição da multimistura, de modo a garantir adequados nívei...

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Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods

Cited by 1,342 publications

References 22 publications

Phenolic compounds and antioxidant activity of hydroalcoholic extracts of wild and cultivated murtilla (Ugni molinae Turcz.)

Phenolic compounds and antioxidant activity of hydroalcoholic extracts of wild and cultivated murtilla (Ugni molinae Turcz.)

Effect of drying techniques on the retention of antioxidant activities of Saskatoon berries

Characterization of a plant dietary supplement (“multimistura”) capacities, used in Mozambique to fight nutritional insecurity

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