Comparison of acridine orange, methylene blue, and Gram stains for blood cultures

Mirrett, Stanley; Lauer, Brian A.; Miller, G A; Reller, L. Barth

doi:10.1128/jcm.15.4.562-566.1982

Cited by 57 publications

(18 citation statements)

References 7 publications

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“…Digital arterial blood pressure was determined with the commercially available Finapres (Ohmeda, Model 2300) device which consists of a finger blood pressure cuff with a built-in infra-red sensitive photocell, a pressure manometer and an automatic unit for cuff inflation, and a monitor with digital display of systolic and diastolic pressure, and heart rate. The apparatus is based on the volume-clamp principle for determination of finger arterial blood pressure, as described by Penaz et al (3) and developed by the Netherlands Research Group (17). The cuff is inflated automatically to a pressure level at which a maximal amplitude of the pulse volume curve is obtained: this usually occurs at mean arterial pressure.…”

Section: Blood Pressure Measurementmentioning

confidence: 99%

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Continuous non-invasive finger blood pressure monitoring in children

et al. 2008

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We evaluated the performance of continuous non-invasive finger arterial pressure measurement using the volume-clamp technique (Finapres). The study was designed to compare finger arterial pressure with brachial blood pressure estimated by the auscultatory method in 217 children (90 boys and 127 girls) aged 4-16 years and in 38 adults (aged 18-45 years). Finger and brachial artery pressure readings were obtained consecutively from the ipsilateral side in the supine position. Fingcr arterial pressure waveforms were recorded in all children except 4 with small and thin fingers. There was good agreement for systolic pressure with only a slight underestimation of 1.9 mmHg and 5.1 mmHg lower for diastolic pressure. This difference most probably reflects inaccuracy of the auscultatory cuff method rather than an error in the Finapres. There was large inter-individual variability in Finapres recordings which might be due to diffcrences in vasomotor tone, as demonstrated by systolic amplification in 5 patients with anorexia. However, Finapres showed a small within-subject variability (3.8 mmHg for systolic and 4.1 mmHg for diastolic pressure) dctermined in 5 patients during phenylephrine infusion, and as good reproducibility as the auscultatory method. These results suggest that finger arterial 4. Kite P, Millar MR, Gorham P, Congden P. Comparison of five tests used in diagnosis of neonatal bacteremia. Arch Dis Child

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Section: Blood Pressure Measurementmentioning

confidence: 99%

“…difference are indicated. Blood pressure (mmHg) 3 11.4 4.5 -8.5 4.5 4 -9.3 3.5 -0.8 3.8 We also examined the reliability of FINA measurement in 5 patients with anorexia nervosa. As shown in Fig.…”

Section: -mentioning

confidence: 99%

Continuous non-invasive finger blood pressure monitoring in children

et al. 2008

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“…Acridine orange, a fluorochrome dye that at low pH allows differentiation of bacteria, which show orange fluorescence, from background material and mammalian cells, which fluoresce green to yellow, is particularly useful for interpretation of smears prepared from thick or purulent material (58,99). It also is more sensitive than the Gram stain for detection of organisms in CSF and other body fluids and in smears prepared from blood culture broth (103,104,126), and in at least one case it allowed detection of Borrelia burgdorferi in sediment of CSF (20). Actinomyces spp.…”

Section: Direct Detection In Smearsmentioning

confidence: 99%

Detection of infection or infectious agents by use of cytologic and histologic stains

1996

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A wide variety of stains are useful for detection of different organisms or, for viruses, the cytopathologic changes they induce, in smears prepared directly from clinical specimens and in tissue sections. Other types of stains, such as hematoxylin and eosin, are used routinely to stain tissue sections and are most valuable for assessing the immunologic response of the host to the invading pathogen. In many cases, the pattern of inflammation provides important clues to diagnosis and helps to guide the selection of additional "special" stains used predominantly for diagnosis of infectious diseases. A stain may be nonspecific, allowing detection of a spectrum of organisms, as do the Papanicolaou stain and silver impregnation methods, or detection of only a limited group of organisms, as do the different acid-fast techniques. Some nonspecific stains, such as the Gram stain, are differential and provide valuable preliminary information concerning identification. Immunohistochemical stains, on the other hand, are specific for a particular organism, although in some cases cross-reactions with other organisms occur. Despite the wealth of information that can be gleaned from a stained smear or section of tissue, however, the specific etiology of an infection often cannot be determined on the basis of only the morphology of the organisms seen; culture data are essential and must be considered in the final diagnosis.

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“…In addition, urethral and cervical smears stained with methylene blue and acridine orange were found to be equally sensitive (4). A recent comparison of acridine orange, methylene blue, and Gram stains for blood cultures demonstrated 38% sensitivity and 99% specificity with both methylene blue and Gram stains (10). The Wayson stain variation has been used successfully to detect plague bacilli in rat secretions (9) and amoebae (1) in CSF.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Wayson variation of a methylene blue staining procedure for the detection of microorganisms in cerebrospinal fluid

Daly¹,

Gooch²,

Matsen³

1985

J Clin Microbiol

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Meningitis of bacterial origin is a severe infection that must receive immediate attention and prompt treatment. We evaluated a basic fuchsin-methylene blue, ethyl alcohol-phenol staining procedure (Wayson stain) and compared it with the Gram stain procedure for evaluation of cerebrospinal fluid. All smears were prepared within 30 min of receiving the specimen and examined without knowledge of the results of the companion smear or culture. Of 546 specimens entered into the study, 84 were culture positive. Based on the culture results, the sensitivity and specificity of Wayson stain were 90 and 98%, respectively, compared with 73% sensitivity and 99% specificity by Gram stain. We observed that Wayson staining is a particularly sensitive method for screening clinical specimens that contain proteinaceous background material. The Wayson staining procedure is a simple and sensitive technique for early detection of meningitis.

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Comparison of acridine orange, methylene blue, and Gram stains for blood cultures

Cited by 57 publications

References 7 publications

Continuous non-invasive finger blood pressure monitoring in children

Continuous non-invasive finger blood pressure monitoring in children

Detection of infection or infectious agents by use of cytologic and histologic stains

Evaluation of the Wayson variation of a methylene blue staining procedure for the detection of microorganisms in cerebrospinal fluid

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