24This study aimed to investigate the effects of active dry yeast (ADY) on rumen 25 microbial composition and slaughter performance of beef cattle. Thirty-two finishing 26 beef cattle (simmental crossbred cattle ♂ × cattle-yaks ♀), with an average body 27 weight of 110 ± 12.85 kg, were randomly assigned to one of four treatments: the low 28 plane of nutrition group (Control), low plane of nutrition group + ADY 2 g/head/d 29 (ADY2), low plane of nutrition group + ADY 4 g/head/d (ADY4) and high plane of 30 nutrition group (HPN). ADY supplementation increased average daily gain (P<0.001), 31 and the carcass weight of ADY4 group had no significant difference with HPN group 32 (P>0.05). The serum glutamic-pyruvic transaminase activity in control and ADY4 33 group was higher than HPN group (P=0.001). The neutral detergent fiber (P=0.022) 34 and acid detergent fiber (P=0.043) digestibility in HPN group was greater than control, 35 but no difference was obtained among ADY2, ADY4 and HPN group (P>0.05). The 36 rumen ammonium nitrogen content in control was greater than ADY2 and ADY4 37 group (P=0.003), and no difference was obtained ADY2, ADY4 and HPN group 38 (P>0.05). The propionic acid content in the rumen in ADY2, ADY4, and HPN group 39 were greater than control group (P<0.001). The simpson (P=0.014) and shannon 40 (P=0.045) indexes in control and HPN group were greater than ADY4 group. At the 41 phylum level, the relative abundance of Firmicutes in the HPN group was higher than 42 ADY4 group (P=0.015). At the genus level, HPN and ADY4 were clustered together, 43 and the relative abundance of Ruminococcaceae UCG-002 in ADY4 group was 44 higher than control and HPN group (P=0.004). In conclusion, supplementation ADY 3 45 4 g/head/d shift the rumen microbial composition of beef cattle fed low plane of 46 nutrition to a more similar level with cattle fed with HPN diet, produced comparable 47 carcass weight with HPN diet. Animal husbandry is now a dynamic and highly developed industry, which is 69 mainly driven by population growth, income growth and urbanization[1]. The rapid 70 development of the animal husbandry is due to the increased demand for livestock 71 products such as meat, milk and eggs, which makes the current production system 72 face challenges. Animal production directly or indirectly contributes 9% of total CO 2 73 emissions, 37% of methane emissions and 65% of nitrous oxide emissions, resulting 74 in global warming[2]. China imports more than 90 million tons of soybean every year. 75 Environmental pollution and shortage of protein feed resources are the main factors 76 that restrict the sustainable development of animal husbandry in China.
77Beef cattle is an important source of high-quality protein and human economy.78 As the global population increasing, the competition between people and livestock 79 like land, water and food is becoming increasingly fierce, especially in beef cattle 80 breeding industry [3]. The elevated feed efficiency in cattle could reduce feed 81 consumption while maintaining hig...