Comparison of Acute Hepatocellular Proliferating Cell Nuclear Antigen Labeling Indices and Growth Fractions, p34<i>cdc2</i> Kinases, and Serum Enzymes in Carbon Tetrachloride-Treated Rats

Monticello, Thomas M.; Barton, Debra S.; Ma, Xiaohong; Babish, John G.; Durham, Stephen K.

doi:10.1177/019262339502300401

Cited by 9 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Monticello et al (1995) demonstrated that a single necrogenic dose of CCl 4 at 120 mg/kg increases a plasma level of ALT from 59 to 2,492 IU/l and percent of hepatocytes in S phase from 1% to 20%. The difference in necrotic and proliferative potentials between CCl 4 and DMF can be seen in absence of both the GST-P-positive foci found in the present study and the altered hepatocellular foci following 13-week inhalation exposure of rats to DMF vapor (Senoh et al, 2003), whereas 13-week inhalation exposure to CCl 4 vapor induced both the GST-P-positive hepatocellular foci and the acidophilic, basophilic and clear altered cell foci (Nagano et al, 2007a).…”

Section: Note2mentioning

confidence: 99%

Enhanced proliferative response of hepatocytes to combined inhalation and oral exposures to N,N-dimethylformamide in male rats

et al. 2008

View full text Add to dashboard Cite

Male Wistar rats were exposed by inhalation to N,N-dimethylformamide (DMF) at 0 (control), 200 or 400 ppm (v/v) for 6 hr/day, 5 days/week and 4 weeks, and each inhalation group received DMF-formulated drinking water at 0, 800, 1,600 or 3,200 ppm (w/w) for 24 hr/day, 7 days/week and 4 weeks. Both the combined inhalation and oral exposures and the single-route exposure through inhalation or ingestion induced centrilobular hypertrophy and single-cell necrosis of hepatocytes, increased plasma levels of alanine aminotransferase (ALT), increased percentage of proliferating cell nuclear antigen (PCNA)-positive hepatocytes without glutathione-S-transferase placental form (GST-P)-positive liver foci, and increased relative liver weight. Those hepatic parameters of the DMF-induced effects were classified into hypertrophic, necrotic and proliferative responses according to the pathological characteristics of affected liver. While magnitudes of the hypertrophic and necrotic responses were linearly increased with an increase in amounts of DMF uptake in the single-route exposure groups, those dose-response relationships tended to level off in the combined-exposure groups. Saturation of the hypertrophic and necrotic responses at high dose levels might be attributed to suppression of the metabolic conversion of DMF to its toxic metabolites. Percentage of PCNA-stained hepatocytes classified as the proliferative response was increased more steeply in the combined-exposure groups than in the single-route exposure groups. It was suggested that the proliferative response of hepatocytes to the combined exposures would be greater than that which would be expected under an assumption of additivity for the component proliferative responses to the single-route exposures through inhalation and ingestion.

show abstract

Section: Note2mentioning

confidence: 99%

Enhanced proliferative response of hepatocytes to combined inhalation and oral exposures to N,N-dimethylformamide in male rats

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Further, recent studies indicate a PCNA interaction with proteins involved in cell cycle progression, which are not a part of the DNA polymerase apparatus but directly affect the DNA synthesis (2). PCNA expression is widely used as a marker of proliferating cells detected by immunocytochemistry (3,4) and by ELISA (4,5). A set of monoclonal antibodies are commercially available for both immunocytochemical detection of PCNA in paraffin-embedded tissue sections or cryostat sections and the identification of the protein by immunoblotting.…”

mentioning

confidence: 99%

“…The DNA amplification strategy has been designated the combined chain reaction (CCR) 1 because it combines polymerase chain reaction (PCR) (2, 3) and ligase chain reaction (LCR) (4,5). In this report, we have applied CCR to site-directed mutagenesis (Fig.…”

mentioning

confidence: 99%

Monoclonal Antibodies against Proliferating Cell Nuclear Antigen Cross-React with the Peroxisomal Multifunctional Protein

Mayer

Beier

et al. 1998

Analytical Biochemistry

View full text Add to dashboard Cite

Alterations in the Growth Factor Signal Transduction Pathways and Modulators of the Cell Cycle in Endocervical Cells from Macaques Exposed to TCDD

Enan

El-Sabeawy

Scott

et al. 1998

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

Comparison of Acute Hepatocellular Proliferating Cell Nuclear Antigen Labeling Indices and Growth Fractions, p34cdc2 Kinases, and Serum Enzymes in Carbon Tetrachloride-Treated Rats

Abstract: We evaluated various biomarkers associated with cell proliferation immediately following insult with the classic hepatotoxicant carbon tetrachloride (CCl 4

Cited by 9 publications

References 28 publications

Enhanced proliferative response of hepatocytes to combined inhalation and oral exposures to N,N-dimethylformamide in male rats

Enhanced proliferative response of hepatocytes to combined inhalation and oral exposures to N,N-dimethylformamide in male rats

Monoclonal Antibodies against Proliferating Cell Nuclear Antigen Cross-React with the Peroxisomal Multifunctional Protein

Alterations in the Growth Factor Signal Transduction Pathways and Modulators of the Cell Cycle in Endocervical Cells from Macaques Exposed to TCDD

Contact Info

Product

Resources

About