Comparison of adiponectin levels and some metabolic parameters in dairy cows with subclinical and clinical ketosis

Abstract

The aim of the presented study was to evaluate the relationship between adiponectin and non-esterified fatty acids (NEFA), β-hydroxybutyric acid (BHBA), glucose, albumin, Gamma-Glutamyl Transferase (GGT), calcium, phosphorus and Blood Urea Nitrogen (BUN) levels in healthy cows and cows suffering clinical or subclinical ketosis in the early postpartum period. A total of 45 Holstein-Fresian dairy cows, consisting of 15 with clinical ketosis, 15 with subclinical ketosis and 15 healthy controls, was used in the study. Selection of animals was based on blood BHBA levels and urine ketone strip results on day 7 after parturition. Blood adiponectin, NEFA, glucose, albumin, GGT, calcium, phosphorus and BUN were also measured on day 7 postpartum. Adiponectin levels were significantly lower in both Clinical Ketosis and Subclinical Ketosis groups compared to the control group. NEFA levels were higher and glucose and calcium levels were lower in both ketosis groups when compared to the control animals. On the other hand, blood albumin, GGT, phosphorus and BUN levels did not differ among study groups. Based on the results of the study, it can be stated that adiponectin may play a role in the pathogenesis of ketosis. This role could be lower milk yield and better energy balance in early postpartum dairy cows with high adiponectin levels due to increased whole body tissue insulin sensitivity..

Metabolomics Integrated with Transcriptomics Reveals a Subtle Liver Metabolic Risk in Dairy Cows Fed Different Crop By‐products

“…To further verify this speculation, subclinical ketosis characterized blood parameters at the sampling time points were analyzed. Significantly higher concentrations of NEFA and BHBA and the concurrent significantly lower blood triacylglycerol as well as the lower blood glucose trend in the RS group when compared with the CS group were indicative of a negative energy balance and liver metabolic disorder, which were consistent with other studies . Even though the concentration of different blood parameters did not go over or below the current subclinical diagnosis threshold, the comparison showed expectable evaluative standards and potential risk .…”

Multi-Omics Reveals Disrupted Immunometabolic Homeostasis and Oxidative Stress in Adipose Tissue of Dairy Cows with Subclinical Ketosis: A Sphingolipid-Centric Perspective

“…Research, including studies on 3T3-L1 cells, has demonstrated that adiponectin release declines when lipolysis is stimulated by β-adrenergic substances [53]. Consistent with findings from Akgul et al [54] and Mann et al [55], cows that exhibited hyperketonemia after calving had diminished adiponectin levels. Furthermore, Kabara et al [56] found that adiponectin exposure notably lowered TNF-α levels in monocytes activated by LPS, thus significantly diminishing their inflammatory activity.…”

The effect of beta-hydroxybutyrate level on other metabolic test parameters

“…Süt sığırlarında yüksek kan NEFA ve BHBA seviyeleri negatif enerji balansının temel göstergeleridir. Bir sürünün ketozis, karaciğer yağlanması ve NED durumunu değerlendirmek için metabolik profil testinde NEFA, BHBA, glikoz, albümin, GGT ve BUN düzeylerinin dikkate alınması gerektiği bildirilmiştir (2). Çalışma gruplarımız arasında Grup-1'in glikoz (p<0,001) ve albümin (p<0,01) düzeylerinin Grup-2'den anlamlı derecede düşük, GGT (p<0,001), AST (p<0,05) ve kalsiyum (p<0,01) düzeylerinin ise anlamlı derecede yüksek olduğu tespit edildi (Tablo 1).…”

“…BHBA dolaşımdaki dominant keton cismidir ve tüm vücutta, plazmada veya serumda nispeten stabil olarak bulunmaktadır (10,11,15,25). Ketozisin tanısı kan BHBA düzeyine dayanmaktadır (2). Süt ineklerinde ketozisin tanısı için farklı BHBA eşik değerleri kullanılmaktadır (12,18,25).…”