Comparison of adipose particle size on autologous fat graft retention in a rodent model

Abstract

particle size on autologous fat graft retention in a rodent model. Plast Aesthet Res 2020;7:8. http://dx. AbstractAim: Unpredictable retention outcomes remain a significant issue in autologous fat grafting procedures. Liposuction cannula variation leads to variability in fat particle size. Recent data suggest that the size of fat particles is closely related to graft healing outcomes; however, this remains a point of contention due to potential confounding variables such as tissue trauma with harvest. The aim of this study was to compare autologous fat grafting outcomes with variable fat particle sizes in an animal model which isolated fat particle size as the primary experimental variable. The overall goal of this work was to determine if reducing fat particle size is an effective method for enhancing graft retention in autologous fat grafting.Methods: The range of fat particle diameter harvested by four common liposuction cannulas was quantified to define relevant small and large particle target diameters. To determine if particle size impacted nutrient and oxygen permeability, small and large particles were incubated in vitro in a spinner flask with an abundance of culture media and vascular endothelial growth factor secretion was measured with enzyme-linked immunosorbent assay. Finally, small and large fat grafts were prepared from subcutaneous mouse fat pads and grafted in syngeneic Balb/CJ mice. Weight and volume retention were evaluated at 1, 4, 8, and 12 weeks. Histological analysis with Masson's trichrome and perilipin Page 2 of 13 Yang et al. Plast Aesthet Res 2020;7:8 I http://dx.immunofluorescent staining was performed. Real-time quantitative polymerase chain reaction was performed for adipogenic, inflammatory and apoptotic genes.Results: The range of fat particle diameters harvested with four commonly used cannulas was 2-7 mm. In vitro studies showed that 5-7-mm particles had significantly increased VEGF secretion normalized to weight, indicating increased tissue hypoxia in these particles compared to 2-4-mm particles. Surprisingly, in vivo comparison in two unique studies showed 2-4-mm and 5-7-mm fat particles had comparable graft retention (P = 0.5329). Masson's trichrome staining revealed increased extracellular matrix and fibrosis in the 5-7-mm particle group (P = 0.0115). Adipocyte survival with perilipin demonstrated comparable viability. Gene expression showed large particles experienced increased inflammation and apoptosis at one week after grafting, but overall there were no significant differences between groups. Conclusion:The ideal fat particle size should be large enough to contain adequate mesenchyme while not so thick as to preclude imbibition. This study suggests that, despite changes in hypoxia and VEGF levels, differing fat particles (2-4-mm and 5-7-mm) can achieve similar graft retention.

Enhanced Fat Graft Viability and Remodeling Using a Helium-based Radiofrequency Device to Prepare the Recipient Site

“…The use of an energy-based device for stimulation of the recipient tissue was investigated for increased retention of fat grafts. Although new fat grafting techniques and animal models show increased retention using smaller fat grafts over a bolus fat graft model, the latter was used to assess if helium-based rf pretreatment could affect the remodeling of fat with large necrotic tissues in the center [ 31 , 32 ]. This model allows for the assessment of the depth of effect, which in this study is shown to be improvement of at least a few hundred microns into the tissue at 4 weeks.…”

A Scoping Review: Autologous Fat Grafting to Improve Volume and Aesthetics of Cleft Lip Deformity

“…Data as to the preferred fat collection method varies; however, one consistency remains in that the preference is for a larger bore cannula during collection because it leads less trauma and cellular rupture of collected adipose tissue [ 15 ]. A 2020 study on adipose particle in fat graft retention in rodent model found that the ideal particle size should be large enough to contain a sufficient amount of mesenchyme but not so large that the particle is unable to properly absorb nutrients [ 16 ]. Comparison between small- (2-4 mm) and large (5-7 mm)-sized particles resulted in similar graft retention.…”

“…Comparison between small- (2-4 mm) and large (5-7 mm)-sized particles resulted in similar graft retention. Even though larger particles underwent early hypoxia and adipocyte loss, the remaining tissue served as a framework for regeneration of new cells [ 16 ].…”