Comparison of adjuvants for a spray freeze-dried whole inactivated virus influenza vaccine for pulmonary administration

Patil, Harshad P.; Murugappan, Senthil; Vries-Idema, Jacqueline de; Meijerhof, Tjarko; Haan, Aalzen de; Frijlink, Henderik W.; Wilschut, Jan; Hinrichs, Wouter L. J.; Huckriede, Anke

doi:10.1016/j.ejpb.2015.04.004

Cited by 18 publications

(16 citation statements)

References 51 publications

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“…We earlier observed that GPI-0100-adjuvanted vaccine administered via the pulmonary route induces IL4-secreting T cells but hardly any IFNγ-secreting T cells ([ 15 ] and unpublished observations). We now evaluated the effect of immunization on the cytokine response found after challenge as this might affect or reflect clinical symptoms.…”

Section: Resultsmentioning

confidence: 95%

“…The immune response to vaccination is determined by the antigen-adjuvant combination and the route of delivery. In previous studies, we showed that GPI-0100 is a potent adjuvant for intramuscular-delivered as well as for pulmonary-delivered subunit or WIV influenza vaccine [ 1 , 14 , 15 ]. In this study, we aimed to get more insight in the immune mechanisms stimulated by GPI-0100 adjuvantation of pulmonary-delivered dry powder vaccines and to determine the effect of the GPI-0100 dose on these immune mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, ISCOMATRIX-adjuvanted pulmonary vaccine also induced long-term antibody and memory responses [ 13 ]. We have earlier shown that immune responses to pulmonary-delivered influenza subunit or WIV vaccine in mice could be significantly enhanced by inclusion of GPI-0100 as adjuvant in both liquid and dry powder vaccine formulations [ 14 , 15 ]. Recently, we investigated in a head-to-head comparison in mice four different adjuvants for a pulmonary-delivered WIV influenza vaccine.…”

Section: Introductionmentioning

confidence: 99%

“…Compared to the TLR ligands Pam3CSK4, MPLA and CpG, GPI-0100 was more potent in inducing mucosal and serum antibodies. Moreover, mice immunized with WIV-GPI-0100 showed reduced lung virus titers after challenge with heterologous influenza strain [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Adjuvantation of Pulmonary-Administered Influenza Vaccine with GPI-0100 Primarily Stimulates Antibody Production and Memory B Cell Proliferation

et al. 2017

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Adjuvants are key components in vaccines, they help in reducing the required antigen dose but also modulate the phenotype of the induced immune response. We previously showed that GPI-0100, a saponin-derived adjuvant, enhances antigen-specific mucosal and systemic antibody responses to influenza subunit and whole inactivated influenza virus (WIV) vaccine administered via the pulmonary route. However, the impact of the GPI-0100 dose on immune stimulation and the immune mechanisms stimulated by GPI-0100 along with antigen are poorly understood. Therefore, in this study we immunized C57BL/6 mice via the pulmonary route with vaccine consisting of WIV combined with increasing amounts of GPI-0100, formulated as a dry powder. Adjuvantation of WIV enhanced influenza-specific mucosal and systemic immune responses, with intermediate doses of 5 and 7.5 μg GPI-0100 being most effective. The predominant antibody subtype induced by GPI-0100-adjuvanted vaccine was IgG1. Compared to non-adjuvanted vaccine, GPI-0100-adjuvanted WIV vaccine gave rise to higher numbers of antigen-specific IgA- but not IgG-producing B cells in the lungs along with better mucosal and systemic memory B cell responses. The GPI-0100 dose was negatively correlated with the number of influenza-specific IFNγ- and IL17-producing T cells and positively correlated with the number of IL4-producing T cells observed after immunization and challenge. Overall, our results show that adjuvantation of pulmonary-delivered WIV with GPI-0100 mostly affects B cell responses and effectively induces B cell memory.

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Section: Resultsmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adjuvantation of Pulmonary-Administered Influenza Vaccine with GPI-0100 Primarily Stimulates Antibody Production and Memory B Cell Proliferation

et al. 2017

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“…Forty-five days after first vaccination, 10 mice from each group were challenged intravenously with 0.1 mL zymotic fluid containing 108 CFU S. aureus. This strain was grown in Nutrient Broth (Oxoid, UK) for 12 h at 37 o C and the bacterial concentration was determined by serial dilution on blood agar plates [24]. The mice were monitored daily for 14 days and the changes of the spirit, appetite and temperature were observed, and the death case was recorded.…”

Section: Evaluation Of Protective Effect Of Vaccinesmentioning

confidence: 99%

Immune Responses in Mice Immunized with Mastitis Multiple Vaccines Using Different Adjuvants

Zhang

Yang

et al. 2018

Acta Scientiae. Vet.

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Background: Bovine mastitis, a serious disease associated with both high incidence and significant economic losses, posing a major challenge to the global dairy industry. The development of vaccines for protection from new infections by mastitis pathogens is of considerable interest to the milk production industry. Vaccination is a common and easy strategy for the control of infectious diseases, and the adjuvants used in the formulation is a critical factor for vaccine efficacy improvement. The main objective of the present study was to evaluate three different adjuvants for their ability to enhance immune responses of mice that vaccinated with Bovine Mastitis Multiple Vaccine.Materials, Methods & Results: The thymus and spleen index, the phagocytic ability of macrophage and the serum antibody levels of mice were detected after vaccination, respectively. The results showed that the thymus index, spleen index, and the phagocytic ability of macrophage of mice in Aluminum group exhibited a significant higher level (P < 0.05) compared with those in the control groups. The difference of the serum antibody levels was significant (P < 0.05) between experimental groups and control group after vaccination. The serum antibody concentration of mice in FIA group was higher compared with other groups and had a longer duration. The antibody concentration of mice in France 206 oil group can not increase as fast as the antibody concentration of Aluminum group, but it can last a longer time at a high level. In conclusion, multiple vaccines mixed with three different adjuvants could enhance the immunity of mice and Freund’s incomplete adjuvant is the best choice for this vaccine.Discussion: Adjuvants play an important role in increasing the efficacy of a number of different vaccines. In this study, three kinds of adjuvants (Aluminum hydroxide, France 206 oil and FIA) were evaluated for their adjuvant effects for multiple vaccine of bovine mastitis in mice and aluminum hydroxide did best as the vaccine adjuvant from the results. Aluminum hydroxide is a universally accepted adjuvant for both human and veterinary vaccines. The goal of vaccination is to generate strong immune response providing protection against infection for a time. Different protective effects will usually obtained by different adjuvants even use same antigen. In this work, FIA, Alum and 206 oil were chosen as adjuvants for inactivated antigens of Streptococcus agalactiae, Streptococcus dysgalactiae and Staphylococcus aureus. The results showed that there was a significantly higher antibody levels in vaccinated mice compared with those in control group. In addition, the mice in France 206 oil and FIA group performed a higher antibody levels and stronger immunity than mice in Aluminum hydroxide groups. These findings suggest that Freund’s incomplete adjuvant (FIA) would be the best candidate as the adjuvant for mastitis multiple vaccines investigated in this study.

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Drying Technologies for Vaccines

Arpagaus

2023

Spray Drying of Vaccines

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Comparison of adjuvants for a spray freeze-dried whole inactivated virus influenza vaccine for pulmonary administration

Cited by 18 publications

References 51 publications

Adjuvantation of Pulmonary-Administered Influenza Vaccine with GPI-0100 Primarily Stimulates Antibody Production and Memory B Cell Proliferation

Adjuvantation of Pulmonary-Administered Influenza Vaccine with GPI-0100 Primarily Stimulates Antibody Production and Memory B Cell Proliferation

Immune Responses in Mice Immunized with Mastitis Multiple Vaccines Using Different Adjuvants

Drying Technologies for Vaccines

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