Comparison of adult liver flukes from highland and lowland populations of Bolivian and Spanish sheep

Valero, M. Adela; Marcos, Marta; Comes, Ana M.; Sendra, Mario; Mas‐Coma, Santiago

doi:10.1017/s0022149x99000578

Cited by 28 publications

(25 citation statements)

References 17 publications

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“…Multidisciplinary studies were undertaken to disentangle how the liver fluke and its lymnaeid snail hosts adapted to the extreme environmental conditions of the high altitude and succeeded in giving rise to high infection rates (MasComa et al 1999c). Intraspecific morphological variability studies showed no significant difference between F. hepatica adult flukes from Bolivia and Spain (Valero et al 1999). Snail anatomy and shell morphology , Samadi et al 1999, DNA sequencing Mas-Coma 1997, Bargues et al 1997) and isoenzymatic studies (JabbourZahab et al 1997) proved that Lymnaea truncatula (Müller, 1774) (Gastropoda: Basommatophora: Lymnaeidae) is the only intermediate host species in the Northern Bolivian Altiplano.…”

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confidence: 99%

Comparative infectivity of Fasciola hepatica metacercariae from isolates of the main and secondary reservoir animal host species in the Bolivian Altiplano high human endemic region

Valero¹,

Mas‐Coma²

2000

FOLIA PARASIT

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Abstract. Fascioliasis due to Fasciola hepatica (Linnaeus, 1758) is an endemic disease on the Northern Bolivian Altiplano, where human prevalences and intensities are the highest known, sheep and cattle are the main reservoir hosts, and pigs and donkeys the secondary ones. Investigations were carried out to study the viability of metacercariae experimentally obtained from eggs shed by naturally infected Altiplanic sheep, cattle, pigs and donkeys. A total of 157 Wistar rats were infected with doses of 5, 10, 20 and 150 metacercariae. Metacercariae aged for different number of weeks were used to analyse the influence of age on their viability. The number of worms successfully developed in each rat was established by dissection. Results obtained show that metacercarial infectivity is dependent upon storage time, being lower when metacercariae are older. The maximum longevity is 31 weeks using doses of 20 metacercariae per rat and 48 weeks with 150 metacercariae per rat, although in the latter case only a very low percentage of worms is recovered. Age-related infectivity of metacercariae from Altiplanic F. hepatica does not significantly differ from that of the liver fluke in lowlands of other countries. Concerning the influence of the isolate according to host species, results indicate that metacercarial viabilities of pig and donkey isolates are similar to the viabilities of metacercariae of sheep and cattle isolates. Thus, pig and donkey have a high transmission potential capacity concerning this aspect. This fact is of great importance for the control of human and animal fascioliasis in this highly endemic zone.

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confidence: 99%

Comparative infectivity of Fasciola hepatica metacercariae from isolates of the main and secondary reservoir animal host species in the Bolivian Altiplano high human endemic region

Valero¹,

Mas‐Coma²

2000

FOLIA PARASIT

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“…These changes may in¯uence the development of a liver¯uke such as F. hepatica, mainly because of its tissue migration and haematophagous diet (Dawes and Hughes 1964;Boray 1969). To ascertain whether geographic isolation and adaptation to high altitude have given rise to a parasite morph divergence (as detected in Altiplanic lymnaeid snails ± see Oviedo et al 1995), dierent morpho-anatomical aspects of liver¯uke adults additional to the characteristics already analysed by Valero et al (1999) are currently being studied. The aim of the present paper is to analyse the results obtained in the studies on uterus development.…”

Section: Introductionmentioning

confidence: 99%

“…In F. hepatica, Neuhaus (1978) has already showed that the length of the uterus in F. hepatica at dierent stages of development is correlated with the size of the body. In the present paper, a comparison is made with the equation described by Valero et al (1991Valero et al ( , 1996Valero et al ( , 1999. To analyse the in¯uence of host species on the development of the liver¯uke uterus at high altitudes, Bolivian Altiplanic liver¯uke populations from three dierent host species (sheep, cattle and pig) were studied.…”

Section: Introductionmentioning

confidence: 99%

Developmental differences in the uterus of Fasciola hepatica between livestock liver fluke populations from Bolivian highlands and European lowlands

2001

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A morphometric study of the uterus area (UA) of Fasciola hepatica adults was carried out with the aid of a computer linked to a stereomicroscopic 3CCD colour video camera using image analysis software. The UA of adult liver flukes found in naturally infected sheep, cattle and pig from the endemic human fascioliasis zone of the northern Bolivian Altiplano highlands was compared with that of flukes found infecting sheep and cattle from Valencia, Spain and cattle from Corsica, France (collectively, European lowlands). Liver fluke UA was examined using an allometric model. A comparison of the allometry of the liver fluke UA in different host species from Bolivia revealed no significant differences. Similarly, no statistically significant differences were found between UA of Valencian and Corsican populations. However, the Bolivian sheep and cattle liver fluke populations proved to have a UA smaller than that of the European populations. These results indicate differences between the liver fluke population of highlands and lowlands, regarding UA. This paper discusses the possible relationship between the characteristic of having a reduced uterine development and the liver fluke adult stage adapting to host populations living at very high altitudes.

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“…To identify eggs from F. hepatica, the parameters described by Boray et al (1983) and Valero et al (1999) were considered. The samples were positive when containing at least one F. hepatica egg.…”

Section: Stool Analysismentioning

confidence: 99%

First Report of Human Fascioliasis in an Endemic Region of Bovine Fascioliasis in Caldas-Colombia

Perez-C

Giraldo-Pinzón

Marín

2016

Vector-Borne and Zoonotic Diseases

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Fascioliasis causes significant economic losses to the cattle industry and is considered a reemerging zoonosis. In Caldas-Colombia, an increase of bovine fascioliasis was detected at the Manizales Municipal Slaughterhouse, which is a potential risk to public health. The ecoepidemiology of human fascioliasis was analyzed in a region of bovine fascioliasis in Caldas-Colombia. The risk factors were studied. Samples were taken from 111 people who were directly related to the bovine milk production process. The immunoglobulin G frequency of Fasciola hepatica was determined in serum. A seriate stool test and a molecular analysis were conducted on those with positive results to look for parasite eggs and DNA, respectively. 6.3% of the samples were positive for the presence of antibodies; none was positive for the presence of eggs, while two samples showed a weak amplification band of the 124-bp DNA fragment of F. hepatica. Fifty-seven percent of the positive samples came from places located at 2026 meters above sea level (masl); 71% of people testing positive had been recently dewormed. Also, 86% had been in contact with cattle and handled grass and excrement. They eat salads and drink untreated water from the springs or ravines of the area. An outbreak of human fascioliasis was detected in Caldas, associated with risk factors for the disease. Clinical trials to confirm the presence of the parasite and implement public health control measures are required.

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Comparison of adult liver flukes from highland and lowland populations of Bolivian and Spanish sheep

Cited by 28 publications

References 17 publications

Comparative infectivity of Fasciola hepatica metacercariae from isolates of the main and secondary reservoir animal host species in the Bolivian Altiplano high human endemic region

Comparative infectivity of Fasciola hepatica metacercariae from isolates of the main and secondary reservoir animal host species in the Bolivian Altiplano high human endemic region

Developmental differences in the uterus of Fasciola hepatica between livestock liver fluke populations from Bolivian highlands and European lowlands

First Report of Human Fascioliasis in an Endemic Region of Bovine Fascioliasis in Caldas-Colombia

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