Comparison of Aeromonas salmonicida resistant and susceptible salmon families: A high immune response is beneficial for the survival against Aeromonas salmonicida challenge

Zhang, Zuobing; Niu, Cuijuan; Storset, Arne; Bøgwald, Jarl; Dalmo, Roy Ambli

doi:10.1016/j.fsi.2010.12.019

Cited by 39 publications

(19 citation statements)

References 42 publications

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“…However, Tlr5 gene expression in the control group was significantly higher after infection. Although L. garvieae is not a flagellated bacterium, and Tlr5 is involved in the recognition of flagellin, previous studies have suggested that some pathogenderived factors other than flagellin may contribute to its activation [32,33].…”

Section: Discussionmentioning

confidence: 99%

Expression of immune-related genes in rainbow trout (Oncorhynchus mykiss) induced by probiotic bacteria during Lactococcus garvieae infection

Pérez‐Sánchez

Balcázar

Merrifield

et al. 2011

Fish & Shellfish Immunology

207

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Section: Discussionmentioning

confidence: 99%

Expression of immune-related genes in rainbow trout (Oncorhynchus mykiss) induced by probiotic bacteria during Lactococcus garvieae infection

Pérez‐Sánchez

Balcázar

Merrifield

et al. 2011

Fish & Shellfish Immunology

207

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“…For example, Sutherland et al (2014) analyzed gene expression profile differences between chum and pink salmon during infections with sea lice ( L. salmonis ) to gain insight into the functional mechanisms underlying the divergent resistance to lice observed in these two species. Zhang et al (2011) took a similar approach to examine the differences between resistant and susceptible salmon families to the pathogen A. salmonicida to highlight the importance of several innate immune response genes. Cofre et al (2014) also examined differential expression of several candidate genes in families with divergent resistance to IPNV.…”

Section: Strategies For Genetic Dissection Of Disease Resistance Traitsmentioning

confidence: 99%

Genetics and genomics of disease resistance in salmonid species

Yáñez¹,

Houston

Newman

2014

Front. Genet.

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Infectious and parasitic diseases generate large economic losses in salmon farming. A feasible and sustainable alternative to prevent disease outbreaks may be represented by genetic improvement for disease resistance. To include disease resistance into the breeding goal, prior knowledge of the levels of genetic variation for these traits is required. Furthermore, the information from the genetic architecture and molecular factors involved in resistance against diseases may be used to accelerate the genetic progress for these traits. In this regard, marker assisted selection and genomic selection are approaches which incorporate molecular information to increase the accuracy when predicting the genetic merit of selection candidates. In this article we review and discuss key aspects related to disease resistance in salmonid species, from both a genetic and genomic perspective, with emphasis in the applicability of disease resistance traits into breeding programs in salmonids.

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“…M -values for these genes were 0.882 ( accD ), 0.891 ( 16S rDNA ), and 1.115 (gapA ). Previous studies have evaluated the stability of potential reference genes in qPCR studies of Atlantic salmon (Olsvik et al, 2005; Løvoll et al, 2009; Zhang et al, 2011). Based on these results, EFN1β, β-actin and the 18S rDNA was chosen to be used as Atlantic salmon reference genes.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, to evaluate the results from the immune parameter analysis, previous reports from bacterial challenge experiment on salmonid fish were used for comparison (Løvoll et al, 2009; Raida and Buchmann, 2009; Ching et al, 2010; Hynes et al, 2011; Zhang et al, 2011; Aykanat et al, 2012; Kvamme et al, 2013). Toll-like receptor 5 (TLR5) plays an essential role in the innate immune defense against bacterial invasion through the recognition of the bacterial protein flagellin followed by the activation of various proinflammatory cytokines (Takeda et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Hynes et al (2011) have reported a significantly increase of TLR5S on day 4 with a fold change of 20–35 when Atlantic salmon was injected with purified flagellin. Another study that performed cohabitant challenge of Atlantic salmon with Aeromonas salmonicida has shown an approximately 2.5 and 10-fold increase of TLR5S after 3 and 14 days, respectively (Zhang et al, 2011). Furthermore, Raida and Buchmann (2009) have challenged rainbow trout fry with Yersinia ruckeri i.p.…”

Section: Discussionmentioning

confidence: 99%

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Expression of Vibrio salmonicida virulence genes and immune response parameters in experimentally challenged Atlantic salmon (Salmo salar L.)

et al. 2013

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The Gram-negative bacterium Vibrio salmonicida is the causative agent of cold-water vibriosis (CV), a hemorrhagic septicemia that primarily affects farmed Atlantic salmon (Salmo salar L.). The mechanisms of disease development, host specificity and adaptation, as well as the immunogenic properties of V. salmonicida are largely unknown. Therefore, to gain more knowledge on the pathogenesis of CV, 90 Atlantic salmon parr were injected intraperitoneally with 6 × 106 CFU of V. salmonicida LFI1238. Samples from blood and spleen tissue were taken at different time points throughout the challenge for gene expression analysis by two-step reverse transcription (RT) quantitative real-time polymerase chain reaction. Out of a panel of six housekeeping genes, accD, gapA, and 16S rDNA were found to be the most suitable references for expression analysis in Vibrio salmonicida. The bacterial proliferation during challenge was monitored based on the expression of the 16S rRNA encoding gene. Before day 4, the concentrations of V. salmonicida in blood and spleen tissue demonstrated a lag phase. From day 4, the bacterial proliferation was exponential. The expression profiles of eight genes encoding potential virulence factors of V. salmonicida were studied. Surprisingly, all tested virulence genes were generally highest expressed in broth cultures compared to the in vivo samples. We hypothesize that this general muting of gene expression in vivo may be a strategy for V. salmonicida to hide from the host immune system. To further investigate this hypothesis, the expression profiles of eight genes encoding innate immune factors were analyzed. The results demonstrated a strong and rapid, but short-lasting innate immune response against V. salmonicida. These results suggest that the bacterium possesses mechanisms that inhibit and/or resist the salmon innate immune system until the host becomes exhausted of fighting the on-going and eventually overwhelming infection.

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Comparison of Aeromonas salmonicida resistant and susceptible salmon families: A high immune response is beneficial for the survival against Aeromonas salmonicida challenge

Cited by 39 publications

References 42 publications

Expression of immune-related genes in rainbow trout (Oncorhynchus mykiss) induced by probiotic bacteria during Lactococcus garvieae infection

Expression of immune-related genes in rainbow trout (Oncorhynchus mykiss) induced by probiotic bacteria during Lactococcus garvieae infection

Genetics and genomics of disease resistance in salmonid species

Expression of Vibrio salmonicida virulence genes and immune response parameters in experimentally challenged Atlantic salmon (Salmo salar L.)

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