Comparison of amniotic fluid matrix metalloproteinase-8 and cathelicidin in the diagnosis of intra-amniotic infection

Myntti, Tarja; Rahkonen, Leena; Pätäri-Sampo, Anu; Tikkanen, Minna; Sorsa, Timo; Juhila, Juuso; Helve, Otto; Andersson, Sture; Paavonen, Jorma; Stefanović, Vedran

doi:10.1038/jp.2016.147

Cited by 11 publications

(11 citation statements)

References 35 publications

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“…Higher concentrations of AF MMP-8 were detected in women with PPROM than in women with intact membranes, as also reported by previous studies [7,17]. However, this difference disappeared when we adjusted for gestational age at sampling, suggesting that gestational age and MIAC status are more important than membrane status.…”

Section: Discussionsupporting

confidence: 85%

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Cervical and Amniotic Fluid Matrix Metalloproteinase-8 and Interleukin-6 Concentrations in Preterm Pregnancies with or without Preterm Premature Rupture of Membranes

et al. 2018

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Introduction: Intra-amniotic inflammation is defined by elevated inflammatory biomarkers in the amniotic fluid (AF), either due to microbial invasion of the amniotic cavity (MIAC) or sterile inflammation. Amniocentesis being an invasive procedure, we wanted to investigate whether elevated matrix metalloproteinase-8 (MMP-8) or interleukin-6 (IL-6) concentrations could be detected from cervical fluid samples. Materials and Methods: This prospective study included 67 women with singleton nondiabetic pregnancies with or without preterm premature rupture of membranes (PPROM) between 22 +0 and 37 +0 weeks of gestation. Simultaneous AF and cervical samples were obtained. Results: In women without PPROM, cervical MMP-8 concentrations correlated with AF MMP-8 concentrations (r S = 0.466, p = 0.002), but cervical IL-6 did not correlate with AF IL-6 (r S = 0.277, p = 0.076). In PPROM cases no correlations were found. Women with MIAC had higher concentrations of AF MMP-8 and AF IL-6 compared to women without MIAC regardless of membrane status. However, only women without PPROM had higher concentrations of cervical MMP-8 in proven MIAC. Conclusion: In women without PPROM, cervical MMP-8 concentration reflects the magnitude of AF MMP-8, thus potentially guiding the selection of patients benefitting from amniocentesis.

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Section: Discussionsupporting

confidence: 85%

“…Over the last two decades, these biomarkers have been extensively studied as predictors of intra-amniotic inflammation (IAI) [13][14][15][16][17][18][19][20]. IAI is characterized by either sterile inflammation or inflammation due to microbial invasion of the amniotic cavity (MIAC) [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Cervical and Amniotic Fluid Matrix Metalloproteinase-8 and Interleukin-6 Concentrations in Preterm Pregnancies with or without Preterm Premature Rupture of Membranes

et al. 2018

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“…MMP-8 belongs to proinflammatory neutrophil based cascade but represents also a known biomarker in this study. The cut-off value for AF-MMP-8 was 41.5 ng/mL, which has shown a sensitivity of 100%, specificity of 69%, PPV of 62%, and NPV of 100% for MIAC in a previous study [ 18 ] and was considered consistent with inflammation. IAI was determined as AF-MMP-8 > 41.5 ng/mL in the presence of MIAC.…”

Section: Methodsmentioning

confidence: 99%

Amniotic Fluid Infection in Preterm Pregnancies with Intact Membranes

et al. 2017

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Introduction. Intra-amniotic infection (IAI) is a major cause of preterm labor and adverse neonatal outcome. We evaluated amniotic fluid (AF) proteolytic cascade forming biomarkers in relation to microbial invasion of the amniotic cavity (MIAC) and IAI in preterm pregnancies with intact membranes. Material and Methods. Amniocentesis was made to 73 women with singleton pregnancies; 27 with suspected IAI; and 46 controls. AF biomarkers were divided into three cascades: Cascade 1: matrix metalloproteinase-8 (MMP-8), MMP-9, myeloperoxidase (MPO), and interleukin-6; Cascade 2: neutrophil elastase (HNE), elafin, and MMP-9; Cascade 3: MMP-2, tissue inhibitor of matrix metalloproteinases-1 (TIMP-1), MMP-8/TIMP-1 molar ratio, and C-reactive protein (CRP). MMP-8 was measured by an immunoenzymometric assay and the others were measured by ELISA. Standard biochemical methods, molecular microbiology, and culture techniques were used. Results. MMP-8, MMP-9, MPO, elafin, and TIMP-1 concentrations were higher in IAI suspected cases compared to controls and also in IAI suspected cases with MIAC compared to those without MIAC when adjusted by gestational age at amniocentesis. All biomarkers except elafin and MMP-2 had the sensitivity of 100% with thresholds based on ROC-curve. Odd ratios of biomarkers for MIAC were 1.2-38 and 95% confidential intervals 1.0-353.6. Conclusions. Neutrophil based AF biomarkers were associated with IAI and MIAC.

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“…MMP-8 was quantified with a solid-phase immunoenzymometric assay (MMP-8 IEMA, Medix Biochemica, Espoo, Finland [ 6 , 7 , 20 ]. This sandwich assay uses two monoclonal antibodies against human MMP-8.…”

Section: Methodsmentioning

confidence: 99%

“…Elevated concentration of decidual phosphorylated IGFBP-1 (phIGFBP-1) has been used as a risk marker for preterm birth [ 4 ] and amniotic fluid non-phosphorylated IGFBP-1 as a marker for fetal membrane rupture [ 5 ]. Further markers include amniotic fluid inflammatory marker matrix metalloproteinase-8 (MMP-8), the higher concentration of which has been associated with preterm birth [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Early pregnancy serum IGFBP-1 relates to lipid profile in overweight and obese women

Mokkala

Juhila²,

Houttu

et al. 2020

Heliyon

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Lower level of insulin-like growth factor-binding protein (IGFBP-1) has been observed in insulin resistance, while higher level of matrix metalloproteinase-8 (MMP-8) has been linked to obesity. The aim here was to study in overweight and obese women, typically manifesting with insulin resistance, whether IGFBP-1 and MMP-8 are related to and reflect systemic low-grade inflammation, metabolism and diet. Fasting serum from overweight and obese pregnant women (n = 100) in early pregnancy were analysed for IGFBP-1, phosphorylated IGFBP-1 (phIGFBP-1) and MMP-8. High-sensitivity CRP and GlycA were used as markers for low grade inflammation. GlycA and lipids were quantified using NMR. IGFBP-1 associated negatively with GlycA, evidenced by higher concentrations in the lowest quartile (median 1.53 (IQR 1.45–1.72)) compared to the highest (1.46 (1.39–1.55)) (P = 0.03). Several lipid metabolites, particularly HDL-cholesterol, correlated inversely with phIGFBP-1 (FDR<0.1). Nutritional status and diet contributed to the levels of IGFBP-1, demonstrated as an inverse correlation with maternal weight (Spearman r = -0.205, P = 0.04) and dietary intake of vitamin A (r = -0.253, P = 0.014) and a direct correlation with dietary intake of polyunsaturated fatty acids (Spearman r = 0.222, P = 0.03). MMP-8 correlated inversely with pyridoxine (r = -0.321, P = 0.002) and potassium (r = -0.220, P = 0.033). Maternal serum IGFBP-1 may contribute to maternal lipid metabolism in overweight and obese women during early pregnancy. These findings may be of importance in identification of metabolic disturbances preceding the adverse metabolic outcomes in pregnancy.

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Comparison of amniotic fluid matrix metalloproteinase-8 and cathelicidin in the diagnosis of intra-amniotic infection

Abstract: The performance of AF-cathelicidin in the prediction of MIAC is comparable to AF-MMP-8.

Cited by 11 publications

References 35 publications

Cervical and Amniotic Fluid Matrix Metalloproteinase-8 and Interleukin-6 Concentrations in Preterm Pregnancies with or without Preterm Premature Rupture of Membranes

Cervical and Amniotic Fluid Matrix Metalloproteinase-8 and Interleukin-6 Concentrations in Preterm Pregnancies with or without Preterm Premature Rupture of Membranes

Amniotic Fluid Infection in Preterm Pregnancies with Intact Membranes

Early pregnancy serum IGFBP-1 relates to lipid profile in overweight and obese women

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