Comparison of an electrochemiluminescence assay in plate format over a colorimetric ELISA, for the detection of ricin B chain (RCA-B)

Guglielmo-Viret, V.; Thullier, Philippe

doi:10.1016/j.jim.2007.08.003

Cited by 39 publications

(24 citation statements)

References 23 publications

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“…The increase in the initial test sample dilution is also beneficial in that it reduces the potential for interference of the test sample matrix. An eightfold increase in sensitivity in the ECL platform compared to the sensitivity of the colorimetric ELISA has been reported by others (9). The assay was shown to have no dilution effect between samples tested at the 1:100,000 and 1:1,000 dilutions and excellent precision at the 1:1,000 dilution (RSD, Ͻ20%).…”

Section: Discussionsupporting

confidence: 51%

“…ECL-based techniques provide an alternative to conventional colorimetric methods (1,4,9), allowing high sensitivity, good reproducibility, and generally low levels of interference from components in complex matrices such as serum or plasma (1,9,26). The Pn ECL assay that we have developed is based on the Meso Scale Discovery (MSD) technology which employs disposable multispot microtiter plates (multiarray plates; MSD, Gaithersburg, MD) that include integrated screenprinted carbon ink electrodes on the bottom of the wells.…”

mentioning

confidence: 99%

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Optimization and Validation of a Multiplex, Electrochemiluminescence-Based Detection Assay for the Quantitation of Immunoglobulin G Serotype-Specific Antipneumococcal Antibodies in Human Serum

Marchese

Puchalski

Miller

et al. 2009

Clin Vaccine Immunol

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Section: Discussionsupporting

confidence: 51%

mentioning

confidence: 99%

Optimization and Validation of a Multiplex, Electrochemiluminescence-Based Detection Assay for the Quantitation of Immunoglobulin G Serotype-Specific Antipneumococcal Antibodies in Human Serum

Marchese

Puchalski

Miller

et al. 2009

Clin Vaccine Immunol

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“…Electroluminescent assay (ELA) platforms such as the Meso Scale Discovery platform have been compared directly with conventional enzyme-linked immunosorbent assays (ELISA) using the same capture and detection antibodies for each assay. Thullier et al have performed the ELA and ELISA comparison studies for ricin B chain and Clostridium botulinum type B neurotoxin (Guglielmo-Viret et al, 2005;Guglielmo-Viret and Thullier, 2007) reporting an 8-fold increase in sensitivity (Guglielmo-Viret and Thullier, 2007) in the ELA format over the ELISA. In addition, the electroluminescent platform has the potential for in-well multiplexing with up to 20 assays printed on the surface to allow for massively parallel assay profile C activation.…”

Section: Introductionmentioning

confidence: 99%

Electroluminescent TCC, C3dg and fB/Bb epitope assays for profiling Complement cascade activation in vitro using an activated Complement serum calibration standard

Vuuren

Bergseth

Mollnes

et al. 2014

Journal of Immunological Methods

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Electroluminescent assays for the complement components C3dg, terminal complement complex (TCC) and factor B (fB) have been developed with capture and detection antibodies to produce detection limits C3dg = 91 ± 9 ng/mL, TCC=3 ± 0.1 ng/mL and fB= 55.7 ± 0.1 ng/mL. The assay performance was assessed against a series of zymosan and heat aggregated IgG (HAIgG) in vitro activations of complement using a calibrated activated complement serum (ACS) as calibration standard. The ACS standard was stable within 20% accuracy over a 6-month period with freeze-thaw cycles as required. Differential activation of the complement cascade was observed for TCC showing a pseudo-first order formation halflife of 3.5 hrs after activation with zymosan. The C3dg activation fragment indicates a 10% total activation for both activation agents. The kinetic-epitope analysis for fB indicates the capture epitope is on the fB/Bb protein fragment and since it becomes covered during the time course.

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“…One of the most important features of this study is using the new technique of Electrochemiluminescence (ECL) compared with the most previous studies in which Enzyme-linked immunosorbent assay (ELISA) method has been used. Moreover, to obtain plasma level of interleukin-6 unlike some previous studies in which the test depends on a test taker, and to obtain the exact level of plasma IL-6, a fully automated machine Elecsys 2010 was used which is not dependent on technicians (7)(8)(9). To achieve more precise and reliable probability of bacterial growth in blood cultures, Bactec culture medium in Becton Dickinson device was used rather than the common blood Agar culture, especially for neonates.…”

Section: Discussionmentioning

confidence: 99%

Predictive Value of Interleukin-6 (IL6) in Term Neonates With Early Sepsis During 2010-2011

Arani

Movahedian

Arani

et al. 2013

Jundishapur J Microbiol

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Background: Neonatal sepsis is one of the main causes of neonatal mortality and morbidity. It breaks out in one to ten cases per 1000 live births with a mortality rate of 10 to 50 percent. However, the rate of hospital admission for neonates suspicious to systemic infections is more than that for neonates with sepsis which is due to the nonspecific signs and symptoms in the neonatal sepsis. Objectives: This study was conducted to measure interleukin 6 (IL-6) plasma level as a sign for early detection of neonatal infection. Materials and Methods:The level of plasma IL-6 and blood culture were checked in all the term neonates suspicious to sepsis admitted to the neonatal intensive care unit of Shahid Beheshti hospital of Kashan during 2010-2011. Positive blood culture was considered as definite sepsis and negative blood culture as suspicious sepsis. Then, the level of IL-6 was compared with the state of blood culture. Premature neonates with low APGAR score, ages more than seven days old, and history of previous admission were excluded from the study due to the probable effect on plasma IL-6 level. Results: Seventy four cases were male and 68 were female. The prevalence of early neonatal sepsis was reported to be 7%. The etiologic bacterial agents were streptococcus in Group B (GBS) and staphylococcus Epidermis (SE). The most common symptom observable in the patients was respiratory distress (38.6%). The average level of IL-6 was 1545.65 pg/mL for the first group (admitted with sepsis signs and symptoms and positive blood culture) and 14.79 pg/mL for the second one (admitted with sepsis signs and symptoms and negative blood culture), while in the control group, it was 11.04 pg/mL. Conclusions: A comparative test indicates that there is a significant difference between the levels of interleukin-6 in three groups (P value = 0.001). This result, not only omits all the probable confounding factors in plasma level of IL-6, but also emphasizes the role of this useful inflammatory marker in detection of neonatal sepsis.

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Comparison of an electrochemiluminescence assay in plate format over a colorimetric ELISA, for the detection of ricin B chain (RCA-B)

Cited by 39 publications

References 23 publications

Optimization and Validation of a Multiplex, Electrochemiluminescence-Based Detection Assay for the Quantitation of Immunoglobulin G Serotype-Specific Antipneumococcal Antibodies in Human Serum

Optimization and Validation of a Multiplex, Electrochemiluminescence-Based Detection Assay for the Quantitation of Immunoglobulin G Serotype-Specific Antipneumococcal Antibodies in Human Serum

Electroluminescent TCC, C3dg and fB/Bb epitope assays for profiling Complement cascade activation in vitro using an activated Complement serum calibration standard

Predictive Value of Interleukin-6 (IL6) in Term Neonates With Early Sepsis During 2010-2011

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