Comparison of an Enzyme-Linked Immunosorbent Assay with an Immunochromatographic Assay for Detection of Lincomycin in Milk and Honey

Abstract

An enzyme-linked immunosorbent assay (ELISA) and an immunochromatographic assay were constructed for the detection of lincomycin (LIN) in both milk and honey samples based on the monoclonal antibody named 5F6. The half-maximum inhibition of ELISA was 0.3 ng/mL after optimizing pH and ionic strength conditions; the limit of detection was 0.07 ng/mL. The cross-reactivity with clindamycin was 0.6%. LIN recovery in spiked milk and honey samples ranged from 84.6% to 115.6% with intra-assay coefficient variations of 1.7-25.4% and inter-assay coefficient variations of 2.7-8.9%. The detection limits were estimated as 2.1 µg/L for milk and 2.1 µg/kg for honey samples. The immunochromatographic assay revealed a LIN cut-off value of 10 ng/mL in PBS, 5 ng/mL in milk, and 120 ng/g in honey, and a visual lower detection limit of 2.5 ng/mL, 1 ng/mL and 30 ng/g in PBS, milk and honey, respectively. The immunochromatographic assay is preferred for large-scale practical application for its simpler pretreatment and satisfied sensitivity compared with ELISA assay.

Development of monoclonal antibody-based colloidal gold immunochromatographic assay for analysis of halofuginone in milk

“…HFG was conjugated to BSA or OVA using the CDI method (Cao et al, 2015). Briefly, a solution of HFG (20 mg) and CDI (16 mg) in N,N ′′ -dimethylformamide (3 ml) was stirred at 60°C for 4 h. It is important that the entire process be kept free of water.…”

Simple, rapid, sensitive, selective and label-free lincomycin detection by using HAuCl4and NaOH

“…Due to the complex elements contained in milk, it was usually selected as the test real sample for antibiotics detection. 16,17 The selected milk is made up of carbohydrates, proteins, fats, citric acid, Na + , etc. According to the reported method, 16 direct 30-fold dilution was performed.…”

“…16,17 The selected milk is made up of carbohydrates, proteins, fats, citric acid, Na + , etc. According to the reported method, 16 direct 30-fold dilution was performed. The spiked samples were obtained by adding Lin with different concentration to the diluted milk.…”

Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine

“…OTA was conjugated to BSA using the CDI method (Cao et al, 2015). Briefly, OTA was mixed with BSA at a 50:1 ratio and dissolved in 2 mL of acetone with CDI.…”