Comparison of an extender containing defined milk protein fractions with a skim milk-based extender for storage of equine semen at 5°C

Pagl, Roland; Aurich, Jörg; Müller-Schlösser, F; Kankofer, Marta; Aurich, Christine

doi:10.1016/j.theriogenology.2006.03.006

Cited by 92 publications

(93 citation statements)

References 20 publications

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“…The viability of the semen was also lower in the Equi-Pro TM samples when compared to all other extenders. According to the manufacturer of Equi-Pro TM (Minitüb, Tiefenbach, Germany) this extender is a modification of the proven Kenney skim milk-glucose formulation [5] and was found to be suitable for storage of cooled stallion semen [13][14][15]. The Equi-Pro TM extender used in this study came from various batches and was stored and prepared according to the manufacturer's instructions and showed a similar effect on all stallions.…”

Section: Discussionmentioning

confidence: 99%

Quality of Raw and of Cold-Stored Semen in Icelandic Stallions

Janett¹,

Sacher²,

Hässig³

et al. 2012

Journal of Equine Veterinary Science

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The aim of the present study was to evaluate the quality of raw and cooled semen in Icelandic stallions. Experiments were performed using seven stallions aged between 3 and 19 years. From each stallion, six ejaculates were collected, and semen quality was determined. Thereafter, the semen was split into eight equal parts and processed with and without centrifugation using the extenders INRA 82-egg yolk, INRA 96, GENT, and Equi-Pro to a final concentration of 30 × 106 sperm/mL. The extended semen was then cooled in an Equitainer, where it was stored for 24 hours, and subsequently refrigerated for another 24 hours at 5°C. Immediately after dilution as well as after 24 and 48 hours storage, sperm motility was analyzed using computer-assisted sperm analyzer, and viability was assessed after dual DNA staining with SYBR-14 in combination with propidium iodide. The results show that the stallion had a significant (P < .05) influence on all variables evaluated in raw semen, and mean (±SEM) values of 43.4 ± 4.3 mL for the volume, 193.0 ± 17.0 × 106 sperm/mL for the concentration, 6.7 ± 0.5 × 109 for total sperm and 73.5 ± 2.1% for total sperm motility, 48.7 ± 2.0% for progressive motility, and 65.3 ± 2.0% for rapid cells were measured. In the cold-stored semen, all variables were significantly (P < .05) influenced by the stallion, extender, and storage time (48 hours). Except for Equi-Pro, all extenders examined were suitable for cooled semen preservation. For storage of more than 24 hours, centrifugation and removal of the seminal plasma were advantageous for all extenders with the exception of Equi-Pro. AbstractThe aim of the present study was to evaluate the quality of raw and cooled semen in Icelandic stallions. Experiments were performed using 7 stallions aged between 3 and 19 years. From each stallion 6 ejaculates were collected and semen quality determined. Thereafter, the semen was split into 8 equal parts and processed with and without centrifugation using the extenders INRA 82-egg yolk, INRA 96 TM , GENT TM and Equi-Pro TM to a final concentration of 30 x 10 6 sperm/mL. The extended semen was then cooled in an Equitainer TM , where it was stored for 24 h, and subsequently refrigerated for another 24 h at 5 °C. Immediately after dilution as well as after 24 and 48 h storage, sperm motility was analyzed using CASA and viability assessed after dual DNA staining with SYBR-14 in combination with propidium iodide. The results show that the stallion had a significant (P < 0.05) influence on all variables evaluated in raw semen and mean (±SEM) values of 43.4±4.3 mL for the volume, 193.0±17.0 x 10 6 sperm/mL for the concentration, 6.7±0.5 x 10 9 for total sperm and 73.5±2.1% for total sperm motility, 48.7±2.0% for progressive motility and 65.3±2.0% for rapid cells were measured. In the cold-stored semen, all variables were significantly (P<0.05) influenced by the stallion, extender and storage time (48 h). Except for Equi-Pro TM , all extenders examined were suitable 2 for cooled semen preservation. For stora...

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Section: Discussionmentioning

confidence: 99%

Quality of Raw and of Cold-Stored Semen in Icelandic Stallions

Janett¹,

Sacher²,

Hässig³

et al. 2012

Journal of Equine Veterinary Science

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“…Hence, the many studies have been continuing on this subject. Although there are many studies on effect of antioxidants, the avaliable information on the antioxidant capacity and lipid peroxidation in frozen ram semen is limited [1][2][3] . The investigations on the selection of the best diluent, antioxidant and dose have been continued by researchers [4][5][6][7][8] .…”

Section: Introductionmentioning

confidence: 99%

Koç Spermasının Dondurulması Üzerine L-Ergothionin, N-asetil sistein ve Sisteinin Etkileri

Yıldız¹,

Öztürkler²,

Ari³

et al. 2015

Kafkas Univ Vet Fak Derg

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The aim of this study was to determine the influence of different doses of L-Ergothioneine (LE), cystein and N-acetylcystein (NAC) on post-thawing semen parameters in rams. Ejaculates collected from four Tushin Rams were evaluated and pooled at 35°C. Semen samples were diluted with skim-milk based extender containing LE (5, 10 mM), Cystein (5, 10 mM), NAC (5, 10 mM) or not containing any antioxidant (control) and loaded to 0.25 ml French straws. Straws were cooled to 5°C for 2 h, frozen in liquid nitrogen vapour (aprox. -120°C) for 15 min and then being stored in liquid nitrogen until thawing process. Straws were thawed in water bath (37°C for 1 min). The percentages of motility, viability, abnormal acrosome, total abnormalities, membrane integrity (hypoosmatic swelling test, HOST) were statistically assessed. Also total antioxidant capacity (TAC) and total oxidatif stres (TOS) were evaluated in samples from replications. It was seen that LE was superior to N-acetylcystein and cystein in motility, viability, defected acrosome, total morphological abnormalities, HOST and TAC, (P<0.05) except cystein in motility. Neverthless, there was not any statistically difference between LE and control groups (P>0.05). It was conlcluded that there was not any beneficial or detrimental effects of LE on post thawing semen parameters in rams while it was determined that cystein and NAC may have been some detrimental effects on post thawing semen parameters in rams. These results warrants future scientific studies on LE, NAC and cystein in ram semen cryopreservation. Keywords: Ram semen, Cryopreservation, L-Ergothioneine, Cystein, N-acetylcystein, Tushin Koç Spermasının Dondurulması Üzerine L-Ergothionin, N-asetil sistein ve Sisteinin Etkileri ÖzetBu çalışmanın amacı, koçlarda L-Ergothionin (LE), sistein ve N-asetil sisteinin (NAC) farklı dozlarının çözüm sonu sperma parametreleri üzerine etkisini belirlemekti. Dört Tuj ırkı koçtan alınan ejakülatlar değerlendirildi ve 35°C'de karışım yapıldı. Sperma örnekleri, LE (5, 10 mM), Sistein (5, 10 mM), NAC (5, 10 mM) içeren veya antioksidan içermeyen (kontrol), yağsız süt temelli sulandırıcıyla sulandırılarak, 0.25 ml'lik payetlere dolduruldu. Payetler 5°C'ye 2 saat süreyle soğutuldu, sıvı azot buharında (yaklaşık -120°C) 15 dak. süre ile donduruldu ve çözme işlemine kadar sıvı azot içinde saklandı. Payetler su banyosunda (37°C'de 1 dak.) çözüldü. Motilite, canlılık, anormal akrozom, toplam anormalite ve membran bütünlüğü (hipoozmotik şişme testi, HOST) yüzdeleri istatistiksel olarak değerlendirildi. Ayrıca, toplam antioksidan kapasite (TAK) ve toplam oksidatif stres (TOS), replikasyonlardan elde edilen örneklerde değerlendirildi. LE'nin motilite (sistein hariç), canlılık, akrozom hasarı, total morfolojik anormalite, HOST ve TAK oranları bakımından, NAC ve sisteine üstünlük sağladığı görüldü (P<0.05). Yine de, LE ve kontrol grubu arasında istatistiksel bir fark yoktu (P>0.05). Sistein ve NAC'ın koçlarda çözüm sonu spermatolojik parametreler üzerine bazı olumsuz etkileri belirlen...

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“…This is especially true in stallions, as spermatozoa have a very limited osmotic threshold [57] which ultimately results in uncontrollable shrinking and swelling of the sperm head causing damage to the semen. Studies have shown that stallion sperm damaged during flash freezing and morphologically abnormal sperm generate greater amounts of ROS [58]. While the fluidity of the plasmalemma is able to tolerate and adjust to these changes without penalty, OS may lead to a loss in viability, poor motility, and/or a decrease in the mitochondrial membrane potential [59].…”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%