Comparison of an HPLC method with a RIA, EMIT and FIA method for the assay of serum gentamicin with extensive statistical evaluation

Hospes, Wobbe; Boskma, R. J.; Brouwers, Jacobus

doi:10.1007/bf01963658

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…An increase in government‐run TDM laboratories, with an improvement in turnaround time can make the process more cost‐effective for the patients. Some institutions have shown the way for the same; others may soon follow them 8,81,82 . In developing countries, where out‐of‐pocket expenditure for medical procedures is high for the patients, the cost may form a barrier to implement the technologies involved in personalised medicine.…”

Section: Resultsmentioning

confidence: 99%

Past, present and future perspectives of therapeutic drug monitoring in India

et al. 2021

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Therapeutic drug monitoring (TDM) is the clinical practice of performing drug assays and interpreting results to maintain constant therapeutic concentrations in patients' bloodstream. Conventional TDM was started way back in the 1960s and served to optimise pharmacotherapy by maximising therapeutic efficacy by evaluating efficacy failure and monitoring drug compliance, while minimising adverse events, in drugs with a narrow therapeutic range. Currently, the scope of TDM has been extended to additional indications which are of importance to India. Apart from the conventional indications, TDM can also help combat drug resistance amongst patients treated with antimicrobials, including anti-tubercular drugs and critically ill patients with compromised pharmacokinetics. TDM is also indicated for patients on antiretroviral drugs under specific clinical scenarios and is of high importance to India. Target concentration intervention (TCI) and apriori TDM (by merging TDM with pharmacogenomics) are emerging fields explored in developed nations. The authors sought to assess the evolution of TDM in India and evaluate the potential impact of newer indications in rationalising pharmacotherapy. In the mid-1980s, TDM was presented to India.Despite showing some initial progress, its use is limited to conventional indications.Its utility is also challenged by cost and higher reliance on conventional prescribing practices. However, the newer indications such as antimicrobial resistance, tuberculosis and HIV, with their high prevalence in developing nations, present an opportunity for the growth of TDM in these countries. Indian clinician's awareness and buoyant demands alongside expert contributions from clinical pharmacologists could widen its scope.

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Section: Resultsmentioning

confidence: 99%

Past, present and future perspectives of therapeutic drug monitoring in India

et al. 2021

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“…Several assays have been developed for the determination of aminoglycoside concentrations (1,6). Recently, the enzyme multiplied immunoassay technique, fluorescence immunoassay, and fluorescence polarization immunoassay have become available (2,3). In general, the analytical accuracy of these techniques has been equivalent (2,3,6,8).…”

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confidence: 99%

“…Recently, the enzyme multiplied immunoassay technique, fluorescence immunoassay, and fluorescence polarization immunoassay have become available (2,3). In general, the analytical accuracy of these techniques has been equivalent (2,3,6,8). However, the accuracy has been questioned in certain clinical situations, e.g., in patients with hyperbilirubinemia (15), hypoalbuminemia (7), and end-stage renal disease (13).…”

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Analytical accuracy of determinations of aminoglycoside concentrations by enzyme multiplied immunoassay, fluorescence polarization immunoassay, and radioimmunoassay in the presence of heparin

O’Connell

Heim²,

Halstenson

et al. 1984

J Clin Microbiol

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The accuracy of gentamicin, netilmicin, and tobramycin concentration determinations by enzyme multiplied immunoassay technique (EMIT; Syva Corp., Palo Alto, Calif.), fluorescence polarization immunoassay (TDx; Abbott Diagnostics, Irving, Tex.), and radioimmunoassay were compared in the presence of 0 to 3,000 USP units of porcine heparin per ml. Gentamicin, netilmicin, and tobramycin concentrations determined by EMIT decreased by 10 and 50% in the presence of 75 and 1,000 USP units/ml, 2 and 5 USP units/mi, and 2 and 7.5 USP units/mi, respectively. Accuracy of the TDx and radioimmunoassay determinations, however, were not affected by the presence of heparin. Blood samples for the determinations of gentamicin, netilmicin, and tobramycin by EMIT should not be collected in evacuated heparinized tubes.

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Evaluation of five immunological methods for the assay of serum netilmicin

Vanremmerden

Brouwers

Berk

et al. 1984

Pharmaceutisch Weekblad Scientific Edition

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Three specific immunological methods for the determination of netilmicin and two enzyme immunoassays for gentamicin were compared for assaying serum netilmicin. The commercial kits we used were Radioimmunoassay (RIA), Substrate Labelled Fluorescence Immunoassay (gentamicin) ( SLFIA ), Homogeneous Enzyme Immunoassay (gentamicin and netilmicin) (EMIT) and Fluorescence Polarization Immunoassay ( FPIA ). After analysing spiked calf serum samples in the range of 0-15.8 mg X 1(-1) netilmicin, extensive statistical analysis was performed. The Performance Index was used to qualify the different methods. Results show that the SLFIA (gentamicin) kit is a very acceptable method for the determination of serum netilmicin levels, well comparable with the EMIT (gentamicin) kit and the EMIT (netilmicin) kit. FPIA and RIA require expensive specialized apparatus so it is only economically feasible when other drugs or substances are also determined on the same apparatus. The abilities and specific needs of each laboratory will play an important role in the choice of a method, because the tested kits are all within the range we need from a clinical point of view.

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Comparison of an HPLC method with a RIA, EMIT and FIA method for the assay of serum gentamicin with extensive statistical evaluation

Cited by 6 publications

References 14 publications

Past, present and future perspectives of therapeutic drug monitoring in India

Past, present and future perspectives of therapeutic drug monitoring in India

Analytical accuracy of determinations of aminoglycoside concentrations by enzyme multiplied immunoassay, fluorescence polarization immunoassay, and radioimmunoassay in the presence of heparin

Evaluation of five immunological methods for the assay of serum netilmicin

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