Comparison of Analytical and Clinical Performances of the Digene HC2 HPV DNA Assay and the INNO-LiPA HPV Genotyping Assay for Detecting High-Risk HPV Infection and Cervical Neoplasia Among HIV-Positive African Women

Abstract

HC2 has lower analytical sensitivity but higher specificity than INNO-LiPA for diagnosing high-grade lesions; the 2 tests presented a comparable clinical sensitivity. HC2 might be suitable for cervical cancer screening in HIV-1-infected African women, but its use in resource-limited settings merits to be further evaluated in comparison with other prevention strategies.

Associations of Human Papillomavirus (HPV) genotypes with high-grade cervical neoplasia (CIN2+) in a cohort of women living with HIV in Burkina Faso and South Africa

“…Measurement of CD4+ cell count was performed using FACScount (Becton-Dickinson, NJ) in both countries. HPV-DNA genotyping of all samples was performed at the virology laboratory of the Gui de Chauliac Teaching Hospital, University of Montpellier, France, using the INNO-LiPA HPV genotyping Extra ® assay (Fujirebio, Courtaboeuf, France) with external quality assurance [11]. HR-HPV types were defined using the current International Agency for Research on Cancer (IARC) classification [12] to include ‘carcinogenic to humans’ (HPV16/18/31/33/35/39/45/51/52/56/58/59) and ‘probable carcinogenic’ (HPV68).…”

Associations of human gene EPB41L3 DNA methylation and cervical intraepithelial neoplasia in women living with HIV-1 in Africa

“…Cervical samples were collected using a Digene cervical sampler (Qiagen, Courtaboeuf, France) for HR-HPV DNA testing and genotyping and DNA methylation assays. HR-HPV testing was performed at baseline and endline at the University of Montpellier virology laboratory using genotyping with the INNO-LiPA HPV genotyping Extra® assay (Innogenetics, Courtaboeuf, France) [22]. The EPB41L3 methylation assays (CpG sites: 438, 427 and 425) were performed at baseline and endline using DNA isolation, bisulfite conversion and quantification of methylation using pyrosequencing (PSQ) assays in single separate reactions, as previously described [23].…”

Diagnostic value of human papillomavirus (HPV) 16 and HPV18 viral loads for the detection of high-grade cervical intraepithelial neoplasia (CIN2+) in a cohort of African women living with HIV

“…the cervical lesions [26]. In addition, HPV DNA detection and typing using the INNO-LiPA HPV genotyping Extra assay (Fujirebio, Courtaboeuf, France) was performed as previously described [27] for all women enrolled in the HARP study at baseline and endline. CD4+ cell counts were measured by flow cytometry using the FACScount system (Beckton Dickinson, Franklin Lakes, NJ, USA).…”